PhycoLink® Streptavidin-R-Phycoerythrin. PhycoPro™ R-PE was conjugated to Streptavidin (Streptomyces avidinii). The conjugate has been purified to remove unreacted components in order to improve sensitivity and reduce non-specific binding. As a final step, the product was filtered using a 0.45 µm membrane and stored in a sterile container. PJRS14 is specifically manufactured as a low background conjugate for applications utilizing bead-based assays such as the Luminex® platform.
Size: 1 mg
Product Code: PJRS14
Q. ProZyme has a number of Streptavidin-R-Phycoerythrin conjugates. How are they different and which one should I use?
A. Our range of Streptavidin-R-Phycoerythrin (SA-RPE) conjugates differ in overall size. We recommend that you test our conjugates to find out which is the best for your system.
Please contact us to set up a sample to test in your application or order the SA-RPE sampler kit, PJ3SX, that contains the following conjugates (0.25mg ea): PJRS20, PJRS301, PJRS34, PJRS25, PJRS27, PJ39S
|Large||Luminex and other bead based assays|
†PJRS301 is our newest conjugate, developed for long-term signal consistency.
Q. What dilution should I use for your SA-RPE conjugates?
A. We don’t have set protocols for our SA-RPE conjugates, as this may depend on your system & application. Please contact us if you are interested in a sample to test. Below are some suggestions.
For our SA-RPE Luminex QC assay we dilute to 10 μg/ml and add 25 μl (0.25 μg) per well. Dilution of SA-RPE to 2-20 μg/ml for addition to the wells is a good starting point for Luminex.
We don’t have specific dilution suggestions for flow, the final conjugate concentration needed in your system should be determined by titration to optimize signal & background. However, dilutions covering the following concentration range might be a decent starting point:
0.01 μg/ml, 0.03 μg/ml, 0.1 μg/ml, 0.3 μg/ml
Product Safety Documentation for PJRS14:
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