Streptavidin-R-Phycoerythrin [PJRS14]

Price:
$ 290.00

Description

PhycoLink® Streptavidin-R-Phycoerythrin

PhycoLink® Streptavidin-R-Phycoerythrin. PhycoPro™ R-PE was conjugated to Streptavidin (Streptomyces avidinii). The conjugate has been purified to remove unreacted components in order to improve sensitivity and reduce non-specific binding. As a final step, the product was filtered using a 0.45 µm membrane and stored in a sterile container. PJRS14 is specifically manufactured as a low background conjugate for applications utilizing bead-based assays such as the Luminex® platform.

Size: 1 mg

Product Code: PJRS14

FAQs

Q. ProZyme has a number of Streptavidin-R-Phycoerythrin conjugates. How are they different and which one should I use?

A. Our range of Streptavidin-R-Phycoerythrin (SA-RPE) conjugates differ in overall size. We recommend that you test our conjugates to find out which is the best for your system. 

Please contact us to set up a sample to test in your application or order the SA-RPE sampler kit, PJ3SX, that contains the following conjugates (0.25mg ea): PJRS20PJRS301PJRS34PJRS25PJRS27PJ39S

 Product Size Application

PJRS301†  PJRS20  PJRS34

Large Luminex and other bead based assays  

PJRS25†† PJRS27

Medium Tetramer
PJ39S Small Flow cytometry

 PJRS301 is our newest conjugate, developed for long-term signal consistency.

PJRS25 is used in the NIH (Emory) Tetramer Core Facility protocol published online, along with our streptavidin-APC (allophycocyanin) conjugate PJ27S. 

Q. What dilution should I use for your SA-RPE conjugates?

A. We don’t have set protocols for our SA-RPE conjugates, as this may depend on your system & application. Please contact us if you are interested in a sample to test. Below are some suggestions.

Luminex

For our SA-RPE Luminex QC assay we dilute to 10 μg/ml and add 25 μl (0.25 μg) per well. Dilution of SA-RPE to 2-20 μg/ml for addition to the wells is a good starting point for Luminex.

Flow cytometry

We don’t have specific dilution suggestions for flow, the final conjugate concentration needed in your system should be determined by titration to optimize signal & background. However, dilutions covering the following concentration range might be a decent starting point:

0.01 μg/ml, 0.03 μg/ml, 0.1 μg/ml, 0.3 μg/ml

MHC tetramers

We suggest using the NIH (Emory) Tetramer Core Facility protocol as a starting point, using PJRS25 (SA-RPE) and PJ27S (SA-APC).

Safety

Product Safety Documentation for PJRS14:

Product/Part No. Description
PJRS14 Streptavidin-R-Phycoerythrin