Gly-X™ Deglycosylation and InstantPC™ Labeling (96-ct) [GX96-201PC]

Price:

Description

The Gly-X™ N-Glycan Rapid Release and Labeling with InstantPC™ kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Other benefits include:

    • Small molecule size for improved chromatographic peak resolution
    • Flexible, high-throughput format: process 1 to 96 samples

    Contains:

      • GX96-100 Gly-X Deglycosylation Module
      • GX96-101 Gly-X InstantPC Labeling Module

      Equipment and reagents provided by user:

        • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
          • Thermocycler (Corning THERM-1001, 110V; THERM-1000, 230V)
          • Note: Two GlykoPrep Heaters, WS0271, can be fitted with VWR 13259-260 Modular Heating Blocks

        Size: 1 kit (96-ct)

        Product Code: GX96-201PC

        Tech Info

        Product Guide Gly-X with InstantPC
        GX96-IPC Gly-X with InstantPC User Manual

        Safety

        Product Safety Documentation for GX96-201PC:

        Product/Part No. Description
        WS0343 Gly-X N-Glycanase (PNGase F) 
        WS0344 Gly-X Digestion Buffer
        WS0345 Gly-X Denaturant
        WS0338 InstantPC Dye
        WS0339 InstantPC Dye Solvent
        Publications

        Development of a 5-Minute Deglycosylation Method and Instant Labeling Dye for High Throughtput N-Glycan Analysis by Mass Spectrometry

        Development of an Instant Glycan Dye for High Throughput Analysis by Mass Spectrometry

        Certificates of Analysis

        This kit contains multiple modules, each with their own Certificate of Analysis, which may be searched for & accessed here.


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