The Gly-X™ N-Glycan Rapid Release and Labeling with InstantPC™ kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Other benefits include:
Equipment and reagents provided by user:
Size: 1 kit (96-ct)
Product Code: GX96-201PC
Q. Can I use the GX96-IPC kit with a Waters vacuum manifold?
A. Yes, if GX200 Gly-X Vacuum Manifold Spacer (Waters Manifold) is used in place of GX100 during the Elute step. GX200 provides the correct distance between the Cleanup Place and Collection Plate when using a Waters vacuum manifold (#186001831). This is critical to avoid crosstalk when eluting labeled N-glycans into the Collection Plate. Please contact ProZyme at firstname.lastname@example.org for further details.
Product Safety Documentation for GX96-201PC:
|WS0343||Gly-X N-Glycanase (PNGase F)|
|WS0344||Gly-X Digestion Buffer|
|WS0339||InstantPC Dye Solvent|
This kit contains multiple modules, each with their own Certificate of Analysis, which may be searched for & accessed here.