The Gly-X™ N-Glycan Rapid Release and Labeling with InstantQ™ kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye. After a simple clean-up step, the glycan samples are ready for analysis on the Gly-Q Glycan Analysis System. With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation. The InstantQ dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Other benefits include:
Equipment and reagents provided by user:
Size: 1 kit (24-ct)
Product Code: GX24-IQ
Q. Can I use more than the recommended upper limit of 40 µg protein per reaction?
A. Maybe, although it will depend on the protein. You would have to test to ensure that labeled glycans from > 40 µg of your protein can be successfully loaded onto the Gly-X Cleanup Matrix without blockage.
Q. My samples are not loading completely onto the Gly-X Cleanup Matrix (Load Step 9, page 9). What is happening?
A. This may be caused by the nature of your protein sample, or by matrix effects caused by the composition of your formulation buffer. This can be addressed by using less protein per reaction, or by buffer exchanging your protein prior to starting the Gly-X protocol.
Product Safety Documentation for GX24-IQ:
|WS0343-24||Gly-X N-Glycanase (PNGase F)|||
|WS0344||Gly-X Digestion Buffer|
|WS0339||InstantPC Dye Solvent|
This kit contains multiple modules, each with their own Certificate of Analysis, which may be searched for & accessed here.