Gly-X™ N-Glycan Rapid Release and Labeling with InstantQ™ Kit (24-ct) [GX24-IQ]

Price:

Description

The Gly-X™ N-Glycan Rapid Release and Labeling with InstantQ™ kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye. After a simple clean-up step, the glycan samples are ready for analysis on the Gly-Q Glycan Analysis System. With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation. The InstantQ dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Other benefits include:

    • Flexible, high-throughput format: process 1 to 24 samples
    • Optimized cleanup removes excess free dye, protein, and other interfering compounds.

    Contains:

      • GX24-300 Gly-X Deglycosylation Module for InstantQ 
      • GX24-301 Gly-X InstantQ Labeling Module
      • GX96-302 Gly-X InstantQ Clean-up Module

      Equipment and reagents provided by user:

        • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
          • Thermocycler (Corning THERM-1001, 110V; THERM-1000, 230V)
          • Note: Two GlykoPrep Heaters, WS0271, can be fitted with VWR 13259-260 Modular Heating Blocks
        • Vacuum manifold (Millipore MSVMHTS00-HTS)
        • Vacuum pump (Millipore WP6211560, 110 V; WP6122050, 220V)
        • Formic acid, MS-grade (Fisher A117-50 recommended)
        • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)

        Size: 1 kit (24-ct)

        Product Code: GX24-IQ

        Tech Info

        Gly-X with InstantQ User Manual 

        FAQs

         

         

        Q. Can I use more than the recommended upper limit of 40 µg protein per reaction?

        A. Maybe, although it will depend on the protein. You would have to test to ensure that labeled glycans from > 40 µg of your protein can be successfully loaded onto the Gly-X Cleanup Matrix without blockage. 

        Q. My samples are not loading completely onto the Gly-X Cleanup Matrix (Load Step 9, page 9). What is happening?

        A. This may be caused by the nature of your protein sample, or by matrix effects caused by the composition of your formulation buffer. This can be addressed by using less protein per reaction, or by buffer exchanging your protein prior to starting the Gly-X protocol.

         

        Safety

        Product Safety Documentation for GX24-IQ:

        Product/Part No. Description
        WS0343-24 Gly-X N-Glycanase (PNGase F) 
        WS0344 Gly-X Digestion Buffer
        WS0345 Gly-X Denaturant
        WS0338 InstantPC Dye
        WS0339 InstantPC Dye Solvent
        WS0333 InstantPC Eluent
        Publications

        Development of a 5-Minute Deglycosylation Method and Instant Labeling Dye for High Throughtput N-Glycan Analysis by Mass Spectrometry

        Development of an Instant Glycan Dye for High Throughput Analysis by Mass Spectrometry

        Certificates of Analysis

        This kit contains multiple modules, each with their own Certificate of Analysis, which may be searched for & accessed here.