Gly-X™ Deglycosylation and InstantPC™ Labeling (24-ct) [GX24-201PC]

Price:

Description

The Gly-X™ N-Glycan Rapid Release and Labeling with InstantPC™ kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. With deglycosylation and labeling carried out in solution phase, the method is simple, rapid and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Other benefits include:

    • Small molecule size for improved chromatographic peak resolution
    • Flexible, high-throughput format: process 1 to 96 samples

    Contains:

      • GX24-100 Gly-X Deglycosylation Module
      • GX24-101 Gly-X InstantPC Labeling Module

      Equipment and reagents provided by user:

        • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
          • Thermocycler (Corning THERM-1001, 110V; THERM-1000, 230V)
          • Note: Two GlykoPrep Heaters, WS0271, can be fitted with VWR 13259-260 Modular Heating Blocks

        Size: 1 kit (24-ct)

        Product Code: GX24-201PC

        Tech Info

        GX96-IPC Gly-X with InstantPC User Manual

        Gly-X with InstantPC Product Guide

        Gly-X InstantDye Automation Product Guide  

        FAQs

        Q. Can I use the GX96-IPC kit with a Waters vacuum manifold?

        A. Yes, if GX200 Gly-X Vacuum Manifold Spacer (Waters Manifold) is used in place of GX100 during the Elute step. GX200 provides the correct distance between the Cleanup Place and Collection Plate when using a Waters vacuum manifold (#186001831). This is critical to avoid crosstalk when eluting labeled N-glycans into the Collection Plate. Please contact ProZyme at info@prozyme.com for further details.

        Safety

        Product Safety Documentation for GX24-201PC:

        Product/Part No. Description
        WS0343-24 Gly-X N-Glycanase (PNGase F)
        WS0344 Gly-X Digestion Buffer
        WS0358 Gly-X Denaturant
        WS0338 InstantPC Dye
        WS0339 InstantPC Dye Solvent
        Publications

        TN4010: Automation of Gly-X N-Glycan Sample Prep with InstantPC and InstantQ Dyes

        TN4009: High Throughput CE-based N-Glycan Analysis for Clone Screening in Biotherapeutic Protein Production 

        TN4008: Rapid N-Glycan Sample Preparation Workflows for Liquid Chromatography and Capillary Electrophoresis Platforms 

        TN4007: High-Throughput Analysis of N-Glycans Released from Biosimilar Glycoproteins 

        TN4005: Comparison of common fluorescent labels for liquid chromatography analysis of released N-linked glycans

        TN4003: Development of an Instant Glycan Labeling Dye for High Throughput Analysis by Mass Spectrometry

        TN4001: Development of a 5-Minute Deglycosylation Method for High Throughput N-Glycan Analysis by Mass Spectrometry

        Certificates of Analysis

        This kit contains multiple modules, each with their own Certificate of Analysis, which may be searched for & accessed here.