GlykoPrep® Rapid N-Glycan Preparation with APTS (96-ct) [GP96NG-APTS]




The GlykoPrep® platform dramatically streamlines glycoprotein sample preparation. From purification to glycan release and labeling, the entire sample prep can be completed in 4 hours.

    • Non-selective, rapid release and recovery of intact N-glycans
    • Flexible, high-throughput format: process 1 to 192 samples per run (2 kits simultaneously)
    • Rapid-Reductive-Amination™ labeling with APTS
    • Purified, fluorescently labeled N-glycans are produced in water and ready for analysis without concentrating or drying
    • Compatible with microplate liquid handling on a broad range of automation platforms

The GlykoPrep Sample Preparation System requires minimal equipment. All you need is a centrifuge with a deep-well plate rotor (minimum 44 mm height), a Starter Labware Kit (AM200) and Heater and Incubation Blocks (GS150).

Size: 1 Kit (96-ct)

Product Code: GP96NG-APTS

Tech Info

GP96NG-APTS Instruction Manual

GP96NG-APTS Microfuge Method Instruction Manual 

GlykoPrep Guidebook  

Posters & Publications

1. Automated Sample Preparation for N-Glycan Analysis by Orthogonal Methods: UPLC and CE

Poster presented at CASSS WCBP, Washington, DC, Jan 28-30, 2014.

2. Orthogonal Methods for Glycoanalysis: CE and UPLC

Poster presented at CASSS CE Pharm, Arlington, VA, Oct 6-10, 2013.
Method details available here.

Product Citations
  1. Szekrényes Á, Park SS, Santos M, Lew C, Jones A, Haxo T, Kimzey M, Pourkaveh S, Szabó Z, Sosic Z, Feng P, Váradi C, de l'Escaille F,Falmagne JB, Sejwal P, Niedringhaus T, Michels D, Freckleton G, Hamm M, Manuilov A, Schwartz M, Luo JK, van Dyck J, Leung PK, Olajos M, Gu Y, Gao K, Wang W, Wegstein J, Tep S, Guttman A.  Multi-Site N-glycan mapping study 1: Capillary electrophoresis - laser induced fluorescence.   2016 Jan 2;8(1):56-64.
  2. Reusch D, Haberger M, Maier B, Maier M, Kloseck R, Zimmermann B, Hook M, Szabo Z, Tep S, Wegstein J, Alt N, Bulau P, Wuhrer M.  Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles--part 1: separation-based methods.   2015;7(1):167-79.

Product Safety Documentation for GP96NG-APTS:

Product/Part No. Description
GS96-RX GlykoPrep Digestion Module 
WS0253 Digestion (RX) Cartridges
WS0256 Immobilization Reagent Set
WS0226 Denaturation Reagent
WS0255 Blocking Reagent
WS0259 Digestion Reagent Set
WS0229 Finishing Reagent
WS0276 25X Digestion Buffer
WS0278 N-Glycanase® (PNGase F)
GS96-APTS Rapid-Reductive-Amination™ APTS Labeling Module 
WS0295 APTS Catalyst
WS0299 APTS Solution
WS0300 Reductant Solution
GS96-C2 GlykoPrep® APTS Cleanup Module
WS0263 Cleanup (CU) Cartridges
WS0301 5x APTS Sample Load Buffer
    Certificates of Analysis

    This kit contains multiple modules, each with their own Certificate of Analysis, which may be searched for & accessed here.