Glyko® Sialidase V™ [GKX-5020]



Releases α(2-3,6,8)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates.


Figure 1: Cleavage specificity for GKX-5020 Sialidase V™.

Native from Vibrio cholerae.

The enzyme is specific for N- acetyl (Neu5Ac) or N-glycolyl (Neu5Gc) neuraminic acid at the non-reducing terminus in α2-3,6 or 8 linkages to various monosaccharides (e.g. galactose, neuraminic acid (NeuAc-NeuAc), N-acetyl-glucosamine, N-acetyl-galactosamine). These are found in a wide range of oligosaccharides, polysaccharides and glycoconjugates such as glycoproteins and glycolipids. The rate of hydrolysis of α2-3 over α2-6 and/or α2-8 linkages is approximately 3-fold [1,2].

Sialidases are also known as neuraminidases.

The purity and broad substrate specificity makes Vibrio cholerae sialidase suitable for oligosaccharide and glycoconjugate structure determination and for probing cell surface molecules (e.g. receptors).

Ships with:
WS0146 5x Reaction Buffer for GKX-5020 [250 mM sodium acetate, 20 mM calcium chloride, 0.5 mg/ml BSA (pH 5.5)

Sterile filtered solution in 50 mM sodium acetate, 154 mM NaCl, 9 mM CaCl2, 0.1% Micr-O-protect, 10 mM EDTA and ~100 μg/ml HSA (pH 5.5)

Reaction Buffer:
5X concentrated buffer which when diluted gives 50 mM sodium acetate pH 5.5, containing 4 mM CaCl2 and 0.1 mg/ml bovine serum albumin.

~83 kD

pH optimum/range:

Unit Definition:
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of methylumbelliferone from MU-NANA [2'-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid] per minute at pH 5.5 and 37°C.

Size: 1 U (200 µl)

Concentration: ≥ 5 U/ml

Product Code: GKX-5020


  1. Ada GL, French EL, Lind PE. Purification and properties of neuraminidase from Vibrio cholerae.  1961 Mar;24:409-25.
  2. Kobata A. Use of endo- and exoglycosidases for structural studies of glycoconjugates.  1979 Nov 15;100(1):1-14.

Product Safety Documentation for GKX-5020:

Product/Part No. Description
Glyko® Sialidase V™ [GKX-5020]