Glyko® α(1-2)-Mannosidase [GKX-5009]

Price:
$ 256.00

Description

Releases α(1-2)-linked mannose residues from the non-reducing terminus of oligosaccharides. Used in exoglycosidase sequencing.

Source:
Aspergillus saitoi.

Specificity:
The enzyme specifically cleaves α(1-2)-linked mannose from the non-reducing terminus of glycans. As such it is extremely valuable in identifying this type of linkage and, in particular, the presence of high mannose-type N-glycans which are digested to the Man-5 structure.

 

Figure 1: Cleavage specificity for GKX-5009 α(1-2)-Mannosidase.

Ships with:
WS0120 5x Reaction Buffer (500 mM sodium acetate pH 5.0)

Reaction Buffer:
5X concentrated buffer which when diluted gives 100 mM sodium acetate pH 5.0.

Formulation:
Lyophilized from water containing 250 μg/ml bovine serum albumin.

MW: ~51 kD

pH optimum/range: 5.0

Unit Definition:
One Unit is defined as the amount of enzyme that will release 1 μmole of mannose from methyl-2-O-α-d-mannopyranosyl-α-d-mannopyranoside per minute at pH 5.0 and 37°C.

Comments:
2 mU is equal to 50μU as previously reported by Glyko, Inc.

Size: 2 mU lyophilized

Product Code: GKX-5009

FAQs

Q. Can I use α(1-2)-Mannosidase to cleave phosphorylated mannose?

A. Information in the literature suggests that the action of α(1-2)-Mannosidase is blocked by phosphorylated mannose [1].

1. Kim MW, Rhee SK, Kim JY, Shimma Y, Chiba Y, Jigami Y, Kang HA.  2004 Mar;14(3):243-51. Epub 2003 Dec 23.

Product Citations
  1. Šesták S, Bella M, Klunda T, Gurská S, Džubák P, Wöls F, Wilson IBH, Sladek V, Hajdúch M, Poláková M, Kóňa J. N-Benzyl Substitution of Polyhydroxypyrrolidines: The Way to Selective Inhibitors of Golgi α-Mannosidase II.  2018 Feb 20;13(4):373-383. 
  2. Thennati R, Singh SK, Nage N, Patel Y, Bose SK, Burade V, Ranbhor RS. Analytical characterization of recombinant hCG and comparative studies with reference product.  2018 Jan 30;12:23-35.
  3. Thak EJ, Kim J, Lee DJ, Kim JY, Kang HA. Structural analysis of N-/O-glycans assembled on proteins in yeasts.  2018 Jan;56(1):11-23.
Safety

Product Safety Documentation for GKX-5009:

Product/Part No. Description
GKX-5009

US-Glyko® α(1-2)-Mannosidase (Aspergillus saitoi)

GB-Glyko® α(1-2)-Mannosidase (Aspergillus saitoi)

 



WS0120

US-5X Reaction Buffer

GB-5X Reaction Buffer