Broad specificity; releases non-reducing terminal α(1-2,3,4,6)-linked fucose from N- and O-glycans. Cleaves α(1-6) core fucose (linked to the innermost GlcNAc of N-glycans) more efficiently than other α-fucose linkages.
The enzyme has broad substrate specificity, cleaving α(1-2,3,4,6)-linked fucose from N- and O-glycans. It cleaves α(1-6) linked fucose on the trimannosyl core of N-linked oligosaccharides more efficiently than other α-fucose linkages. The fine specificity of the enzyme is complicated since the aglycon portion of the substrate significantly influences the substrate kinetics. The rate of cleavage is lower with increasing oligosaccharide size and complexity.
Figure 1: Cleavage specificity for GKX-5006 α(1-2,3,4,6) Fucosidase.
Bovine derived α-fucosidase has been extensively used in the sequence analysis of N-glycans. It has also been used for analysis and modification of glycoconjugates including blood group oligosaccharides and glycolipids.
WS0122 Buffer [500 mM sodium citrate/phosphate pH 6.0, 250 ug/ml BSA]
5X concentrated buffer which when diluted gives 100 mM sodium citrate phosphate pH 6.0.
Lyophilized from 20 mM sodium citrate phosphate, 0.25 mg/ml BSA (pH 6.0)
MW: 210-220 kD
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP-α-fucopyranoside per minute at pH 6.0 and 37°C.
Size: 500 mU lyophilized
Product Code: GKX-5006
Q. Can I use this enzyme to remove core (α-1,6 linked) fucose from the N-glycans of intact glycoproteins such as IgG?
A. Using bovine kidney fucosidase (GKX-5006) to remove core fucose from an N-glycan while the glycan is still attached to an intact IgG is likely to be difficult, due to steric hindrance. For example, bovine kidney fucosidase has been shown to be ineffective at removing core fucose from the N-glycans of intact rituximab .
Absence of core fucose on the N-glycans of therapeutic antibodies enhances antibody-dependent cellular toxicity (ADCC) , and this has spurred interest in other approaches to controlling antibody fucosylation , including the use of cell lines with altered fucosylation pathways.
Product Safety Documentation for GKX-5006:
|GKX-5006||Glyko® α(1-2,3,4,6) Fucosidase (bovine kidney)|||