Releases non-reducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues.
The enzyme exhibits a broad specificity, cleaving non-reducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. This enzyme is very useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with β N-acetylhexosaminidase from S. pneumoniae (GK80050).
We also offer this enzyme at a higher concentration - see GKX-5023. Higher enzyme concentrations may be necessary to remove bisecting β(1-4)-linked GlcNAc residues in complex N-glycans.
Figure 1: Cleavage specificity for GKX-5003 β N-Acetylhexosaminidase.
Useful for the structural analysis of isolated glycans and glycoconjugates.
WS0109 5x Incubation Buffer [500 mM sodium citrate phosphate (pH 5.0)]
5X concentrated buffer which when diluted gives 100 mM sodium citrate/phosphate pH 5.0.
20 mM sodium citrate phosphate, 0.25 mg/ml BSA, 0.02% sodium azide (pH 4.0)
MW: 100 - 110 kDa
For glucosaminide 5.0 - 6.0
For galactosaminide 3.5 - 4.0
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP N-acetyl-β-D-glucosaminide per minute at pH 5.0 and 37°C.
Size: 5 U in 100 µl
Concentration: ≥ 50 U/ml
Product Code: GKX-5003
Product Safety Documentation for GKX-5003:
Subscribe to our newsletter and always be the first to hear about what is happening.
Copyright ©ProZyme 2017