GlykoPrep® 2-AB Verification Standard [GKSB-965]

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Description


The 2-AB-Verification Standard consists of a library of Human IgG N-linked glycans whose reducing termini are derivatized with the fluorescent dye, 2-AB (2-aminobenzamide). The labeled glycans were released using GlykoPrep® from the same lot of material that is packaged in the Human IgG Glycoprotein control vials (WS0162) included in the packaging. The library is intended for use as a verification standard for the GlykoPrep platform.

Please contact us for a representative Certificate of Analysis.

Size: 200 pmol

Product Code: GKSB-965

Structures

Below is a table indicating the N-glycans typically contained in this library. Due to variability in starting material, actual glycans identified in each lot may be subject to change. If you would like more information about our current lot(s), please contact us.

Table 1 - N-Glycans of Human IgG N-Linked Glycan Library (GKSB-965)

Glycan Identification ProZyme Common Oxford (New) [1] Structure [2]
Asialo-, agalacto- biantennary with core fucose NGA2F G0F F(6)A2
Asialo-, agalacto- biantennary with core fucose and with bisecting N-Acetylglucosamine NGA2FB G0FB F(6)A2B
Asialo-, monogalactosylated biantennary with core fucose NA2G1F G1F[6] F(6)A2[6]G(4)1
Asialo-, mono-galactosylated biantennary with core fucose NA2G1F G1F[3] F(6)A2[3]G(4)1
Asialo, mono-galactosylated biantennary, core substituted with fucose and bisecting N-Acetylglucosamine NA2G1FB G1FB F(6)A2[6]BG(4)1
Asialo, galactosylated biantennary NA2 G2 A2G(4)2
Asialo, galactosylated biantennary with core fucose NA2F G2F F(6)A2G(4)2
Asialo, galactosylated biantennary with core fucose and bisecting N-Acetylglucosamine NA2FB G2FB F(6)A2BG(4)2
Mono-α(2-6)-sialylated, mono-galactosylated biantennary with core fucose NA2G1FS1 G1FS1 F(6)A2[3]G(4)1S(6)1
Mono-α(2-6)-sialylated, galactosylated biantennary with core fucose A1F G2FS1 F(6)A2G(4)2S(6)1


Structure Key [2]:

Structures drawn using GlycoWorkbench [3]. 

  1. GlycoBase 3.2.4. [Internet]. Blackrock, Ireland: National Institute for Bioprocessing Research and Training (NIBRT); c2012. Guide to the abbreviations used to identify 2AB labelled N-glycans; [cited 2016 Apr 19].  Available from: www.glycobase.nibrt.ie/glycobase/documents/abbreviations.pdf
  2. Harvey DJ, Merry AH, Royle L, Campbell MP, Dwek RA, Rudd PM. Proposal for a standard system for drawing structural diagrams of N- and O-linked carbohydrates and related compounds.  2009 Aug;9(15):3796-801
  3. Ceroni A, Maass K, Geyer H, Geyer R, Dell A, Haslam SM. GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans.  2008 Apr;7(4):1650-9.