The Glyko® InstantPC-α(2-6) Sialylated Triantennary Library consists of triantennary oligosaccharides containing α(2-6) linked sialic acid. The reducing termini are derivatized with the fluorescent dye, InstantPC.
Please contact us for a representative Certificate of Analysis.
The α(2-6) sialic acid linkage is the predominant linkage type on human intravenous immunoglobulin (IVIG) IgG Fc N-glycans . This differs from glycoproteins produced in Chinese hamster ovary (CHO) cells, where N-glycans are α(2-3)-sialylated . Depending on the separation method, it may be possible to resolve α(2-3) and α(2-6) sialic acid linkage isomers. For example, α(2-3)-sialylated N-glycans are known to have a shorter HILIC retention time than isomeric N-glycans with α(2-6) sialic acid linkages [3,4]. Sialic acid linkage position may also be determined by exoglycosidase digests with Sialidase S (GK80021), which releases non-reducing terminal α(2-3)-linked sialic acid, and sialidase A (GK80040) which releases α(2-3,6,8,9)-linked sialic acid.
Size: ~25 injections
Product Code: GKPC-263
The table below indicates the N-glycans typically contained in this library in order of HILIC retention time (shortest to longest) using QC HILIC methods. Due to variability in starting material, actual glycans identified in each lot may be subject to change. If you would like more information about our current lot(s), please contact us.
N-Glycans of InstantPC-α(2-6) Sialylated Triantennary Library:
|Glycan Identification||ProZyme||Oxford (New) ||Structure |
|Asialo, galactosylated triantennary||NA3||A3G(4)3|
|Mono-α(2-6)-sialylated, galactosylated triantennary||NA3S1||A3G(4)3S(3)1|
|Di-α(2-6)-sialylated, galactosylated triantennary||NA3S2||A3G(4)3S(3)2|
|Tri-α(2-6)-sialylated, galactosylated triantennary||A3||A3G(4)3S(3)3|
Structure Key :
Structures drawn using GlycoWorkbench.
See also our sialylated libraries that contain α(2-3) or α(2-6)-linked sialic acid:
α(2-3)-linked sialic acid:
α(2-6)-linked sialic acid:
Product Safety Documentation for GKPC-263:
|GKPC-263||Glyko® 2-AB-α(2-6) Sialylated Triantennary Library|