The high affinity of streptavidin for biotin precludes using streptavidin-agarose for binding and eluting biotinylated proteins, because there is no way to elute the proteins without denaturing them and the streptavidin. Streptavidin-agarose has been used for affinity purification, by binding a biotinylated affinity ligand. Examples include binding biotinylated antibodies to streptavidin-agarose for immunoaffinity purification of antigens.
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