Advance your workflow with traditional 2-AB labeling

We are proud to announce the launch of Gly-X™ Rapid N-Glycan Release and Labeling with 2-AB Express.  The new on-matrix 2-AB Express labeling method developed by ProZyme eliminates the lengthy dry down step of traditional 2-AB protocols.  Combined with rapid deglycosylation and cleanup, samples are ready for analysis in ~ 2.5 hours total time

Sample Prep

  • Traditional reductive amination 2-AB chemistry
  • 5-minute in-solution deglycosylation
  • 1 hour on-matrix 2-AB labeling
  • Rapid vacuum plate-based cleanup


  • Higher total signal compared to other methods
  • Reliable, data consistency across methods
  • Collection plate loads directly into UPLC auto-samplers
  • Extensive collection of 2-AB labeled N-Glycan libraries and standards

Other labels available for the Gly-X Platform: 

  • InstantPC, a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. 
  • InstantAB, ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. 
  • InstantQ, a negatively charged N-glycan label for capillary electrophoresis (CE), designed for use with our Gly-Q N-glycan analysis platform.

Request a Gly-X demo or ask a question