Citations - Other Glycobiology Enzymes

Rabbit antithymocyte globulin-induced serum sickness disease and human kidney graft survival

Couvrat-Desvergnes, G; Salama, A; Le Berre, L; Evanno, G; Viklicky, O; Hruba, P; Vesely, P; Guerif, P; Dejoie, T; Rousse, J; Nicot, A; Bach, JM; Ang, E; Foucher, Y; Brouard, S; Castagnet, S; Giral, M; Harb, J; Perreault, H; Charreau, B; Lorent, M; Soulillou, JP; J. Clin. Invest.; 2015; 125(12): 4655-65.

Snippet: “…The digestion mixture was separated by reversed-phase HPLC. Pooled glycopeptide fractions were further digested with β-galactosidase from bovine testes (ProZyme). All MS analyses were performed …”

Product: β-galactosidase from bovine testes

Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response

Chen, JT; Chen, CH; Ku, KL; Hsiao, M; Chiang, CP; Hsu, TL; Chen, MH; Wong, CH; Proc. Natl. Acad. Sci. U.S.A.; 2015; 112(42): 13057-62.

Snippet: “…To detect the presence of terminal α-galactose, full-length B7-H3 glycopeptide samples were treated with α-galactosidase (ProZyme) at 37 °C overnight in a 10-μL reaction under the conditions suggested by the vender...”

Product: α-galactosidase

Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast

Halim, A; Larsen, IS; Neubert, P; Joshi, HJ; Petersen, BL; Vakhrushev, SY; Strahl, S; Clausen, H; Proc. Natl. Acad. Sci. U.S.A.; 2015; 112(51): 15648-53.

Snippet: “…The remaining 50% of each IEF fraction was digested at 37 °C ON with 30 U/mL jack bean α-mannosidase (ProZyme) in 100 mM sodium acetate, 2 mM Zn2+, pH 5 before analysis by nLC-MS/MS...”

Product: jack bean α-mannosidase

Site-specifically labeled CA19.9-targeted immunoconjugates for the PET, NIRF, and multimodal PET/NIRF imaging of pancreatic cancer

Houghton, JL; Zeglis, BM; Abdel-Atti, D; Aggeler, R; Sawada, R; Agnew, BJ; Scholz, WW; Lewis, JS; Proc. Natl. Acad. Sci. U.S.A.; 2015; 112(52): 15850-5.

Snippet: “…The resultant antibody solution was supplemented with 40 µL of β-1.4-galactosidase [from Streptococcus pneumoniae (2 mU/µL), obtained from ProZyme] and placed in an incubator at 37 °C for 6 h...”

Product: β-1.4-galactosidase [from Streptococcus pneumoniae]

The functional O-mannose glycan on α-dystroglycan contains a phospho-ribitol primed for matriglycan addition

Praissman, JL; Willer, T; Sheikh, MO; Toi, A; Chitayat, D; Lin, YY; Lee, H; Stalnaker, SH; Wang, S; Prabhakar, PK; Nelson, SF; Stemple, DL; Moore, SA; Moremen, KW; Campbell, KP; Wells, L; Elife; 2016; 5: .

Snippet: “…Liquid chromatography and mass spectrometry systems were purchased from Thermo Scientific (Waltham, MA). Glycosidases were purchased from ProZyme, Hayward, California. Spin columns were purchased from Nest group (Southborough, MA). …”

Product: Glycosidases

Glycomic Analysis of Life Stages of the Human Parasite Schistosoma mansoni Reveals Developmental Expression Profiles of Functional and Antigenic Glycan Motifs

Smit, CH; van Diepen, A; Nguyen, DL; Wuhrer, M; Hoffmann, KF; Deelder, AM; Hokke, CH; Mol. Cell Proteomics; 2015; 14(7): 1750-69.

Snippet: “… For treatment with β(1–4,6)-galactosidase from jack bean (JBG) (227.3 mU) (ProZyme, Hayward , California) 250 mm sodium citrate pH 4.0 was used. All exoglycosidase digestions were performed at 37 °C for 24 h. Immunofluorescence Microscopy. …”

Product: β(1–4,6)-galactosidase from jack bean

Multistep Fractionation and Mass Spectrometry Reveal Zwitterionic and Anionic Modifications of the N- and O-glycans of a Marine Snail

Eckmair, B; Jin, C; Abed-Navandi, D; Paschinger, K; Mol. Cell Proteomics; 2016; 15(2): 573-97.

Snippet: “… Either α-mannosidases (jack bean from Sigma, Aspergillus α1,2-specific from ProZyme, Hayward, California, Xanthomonas α1,2/3-specific from NEB or Xanthomonas α1,6-specific from NEB (Ipswich, MA)), β-mannosidase (H. pomatia from Sigma), α-fucosidases (bovine kidney α1,6 …”

Product: α-mannosidases (Aspergillus α1,2-specific)

More Than Just Oligomannose: An N-glycomic Comparison of Penicillium Species

Hykollari, A; Eckmair, B; Voglmeir, J; Jin, C; Yan, S; Vanbeselaere, J; Razzazi-Fazeli, E; Wilson, IB; Paschinger, K; Mol. Cell Proteomics; 2016; 15(1): 73-92.

Snippet: “… Further analysis by MALDI-TOF MS and MS/MS was performed after treatment with α-mannosidases (jack bean from Sigma, Aspergillus α1,2-specific from ProZyme, Xanthomonas mannosidase II or III, respectively α1,6- and α1,2/3- specific, from New England Biolabs, Ipswich …”

Product: α-mannosidases (Aspergillus α1,2-specific)

Label-free glycoprofiling with multiplex surface plasmon resonance: a tool to quantify sialylation of erythropoietin

Geuijen, KP; Halim, LA; Schellekens, H; Schasfoort, RB; Wijffels, RH; Eppink, MH; Anal. Chem.; 2015; 87(16): 8115-22.

Snippet: “… β-N-acetylhexosaminidase (2U), α-mannosidase (0.5U), and β-mannosidase (0.5U). All exoglycosidases were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands) except (1-4,6)-galactosidase which was purchased from ProZyme (Hayward, Califirnia)..."

Product: (1-4,6)-galactosidase

A Method for In-Depth Structural Annotation of Human Serum Glycans That Yields Biological Variations

Song, T; Aldredge, D; Lebrilla, CB; Anal. Chem.; 2015; 87(15): 7754-62.

Snippet: “…β(1–4) galactosidase and α(1-3,4) fucosidase were purchased from ProZyme, Hayward, California. Sodium borohydride was purchased from Sigma-Aldrich. All reagents were of analytical or HPLC grade…”

Product: β(1–4) galactosidase and α(1-3,4) fucosidase

Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris

De Meyer, T; Laukens, B; Nolf, J; Van Lerberge, E; De Rycke, R; De Beuckelaer, A; De Buck, S; Callewaert, N; Depicker, A; Plant Biotechnol. J.; 2015; 13(7): 938-47.

Snippet: “…The exoglycosidases used were recombinant Trichoderma reesei α-1,2-mannosidase (Maras et al., 2000), jack bean α-1,2/3/6-mannosidase (Sigma), ß-N-acetylhexosaminidase (prozyme) and glucosidase II (a gift from Dr. Terry Butters, Glycobiology Institute, Department of Biochemistry, Oxford, UK). The latter was used as described previously (De Pourcq et al., 2012), and the other reactions were performed in 20 mm ammonium acetate...”

Product: ß-N-acetylhexosaminidase

N-Glycosylation of Serum IgG and Total Glycoproteins in MAN1B1 Deficiency

Saldova, R; Stöckmann, H; O'Flaherty, R; Lefeber, DJ; Jaeken, J; Rudd, PM; J. Proteome Res.; 2015; 14(10): 4402-12.

Snippet: “… α(2,6)- linkage. figure Figure 2. Glycan nomenclature. Exoglycosidase Digestion All enzymes were purchased from ProZyme, California. The 2AB-labeled glycans were …”

Product: Exoglycosidase Digestion All enzymes

Elevation of CA19-9-Related Novel Marker, Core 1 Sialyl Lewis A, in Sera of Adenocarcinoma Patients Verified by a SRM-Based Method

Tanaka-Okamoto, M; Yabu, M; Mukai, M; Takahashi, H; Fujiwara, Y; Ohue, M; Kamada, Y; Miyoshi, E; Miyamoto, Y; J. Proteome Res.; 2016; 15(1): 152-65.

Snippet: “…0.4 U/mL β1,4-galactosidase from Streptococcus pneumonia (ProZyme, California) in 100 mM sodium citrate buffer, pH 6.0, for 2 h at 37 °C; (c) 10 U/mL of β-N-acetylhexosaminidase …”

Product: β1,4-galactosidase from Streptococcus pneumonia

Quantitative GSL-glycome analysis of human whole serum based on an EGCase digestion and glycoblotting method

Furukawa, J; Sakai, S; Yokota, I; Okada, K; Hanamatsu, H; Kobayashi, T; Yoshida, Y; Higashino, K; Tamura, T; Igarashi, Y; Shinohara, Y; J. Lipid Res.; 2015; 56(12): 2399-407.

Snippet: “…β1,4-Galactosidase (Streptococcus pneumonia) and β1-2,3,4,6-N- acetylglucosaminidase (S. pneumonia) were from Calbiochem (San Diego, California). Glyco ® α(1-2,3,4,6) fucosidase was from ProZyme, Hayward, California. …”

Product: α(1-2,3,4,6) fucosidase

Shiga toxin glycosphingolipid receptors of Vero-B4 kidney epithelial cells and their membrane microdomain lipid environment

Steil, D; Schepers, CL; Pohlentz, G; Legros, N; Runde, J; Humpf, HU; Karch, H; Müthing, J; J. Lipid Res.; 2015; 56(12): 2322-36.

Snippet: “… A volume of 100 μl of β(1-4)-galactosidase (ProZyme Inc., Hayward, California; catalog number GKX-5013), dissolved in 20 mM sodium citrate phosphate/150 mM NaCl buffer (pH 4.0) and equivalent to 0.5 U of enzyme, was added and incubated for 48 h at 37°C. In a second approach …”

Product: β(1-4)-galactosidase

The use of lectin microarray for assessing glycosylation of therapeutic proteins

Zhang, L; Luo, S; Zhang, B; MAbs; 2016; 8(3): 524-35.

Snippet: “…Glycosyltransferases b1-4-galactosyltransferase (b1-4GalT) and a2-6-sialyltransferase (a2-6SiaT) were purchased from ProZyme...”

Product: Glycosyltransferases b1-4-galactosyltransferase (b1-4GalT) , a2-6-sialyltransferase (a2-6SiaT)

Unraveling the novel structure and biosynthetic pathway of O-linked glycans in the Golgi apparatus of the human pathogenic yeast Cryptococcus neoformans

Lee, DJ; Bahn, YS; Kim, HJ; Chung, SY; Kang, HA; J. Biol. Chem.; 2015; 290(3): 1861-73.

Snippet: “… 2-AA-labeled O-glycans were reacted with 1 μl of jack bean mannosidase (150 milliunits/μl; Sigma), α1,2-mannosidase (α1,2-MNS; 0.1 milliunits/μl; ProZyme), or α1,6-mannosidase (α1,6-MNS; 40 units/μl; New England Biolabs) in 20 mm ammonium acetate buffer (pH 5.0) for …”

Product: α1,2-mannosidase

Septin dynamics are essential for exocytosis

Tokhtaeva, E; Capri, J; Marcus, EA; Whitelegge, JP; Khuzakhmetova, V; Bukharaeva, E; Deiss-Yehiely, N; Dada, LA; Sachs, G; Fernandez-Salas, E; Vagin, O; J. Biol. Chem.; 2015; 290(9): 5280-97.

Snippet: “… Deglycosylation of sec-β1 present in cell lysates or culture media was performed by using peptide N-glycosidase F from Flavobacterium meningosepticum (New England Biolabs Inc., Ipswich, MA) or endoglycosidase H from Streptomyces plicatus (Glyco-ProZyme Inc., Hayward …”

Product: endoglycosidase H from Streptomyces plicatus

Influenza Virus Hemagglutinin Glycoproteins with Different N-Glycan Patterns Activate Dendritic Cells In Vitro

Liu, WC; Lin, YL; Spearman, M; Cheng, PY; Butler, M; Wu, SC; J. Virol.; 2016; 90(13): 6085-96.

Snippet: “…The 2-AB glycans were digested with jack bean α-mannosidase (ProZyme) prior to another round of HILIC to confirm their structures. A 2-AB-labeled dextran ladder standard …”

Product: jack bean α-mannosidase

Recombinant mucin-type fusion proteins with Galα1,3Gal substitution as Clostridium difficile toxin A inhibitors

Cherian, RM; Jin, C; Liu, J; Karlsson, NG; Holgersson, J; Infect. Immun.; 2016;: .

Snippet: “…Released O-glycans were 192 treated with green coffee bean α-galactosidase (2 mU; ProZyme, Hayward, California) at 37℃ 193 overnight in 100 mM sodium citrate/phosphate, pH 6.0. 194 195 …”

Product: α-galactosidase

Targeted release and fractionation reveal glucuronylated and sulphated N- and O-glycans in larvae of dipteran insects

Kurz, S; Aoki, K; Jin, C; Karlsson, NG; Tiemeyer, M; Wilson, IB; Paschinger, K; J Proteomics; 2015; 126: 172-88.

Snippet: “… either β-galactosidase (either Xanthomonas manihotis β1,3-galactosidase from NEB or recombinant Aspergillus niger lacA β1,4-galactosidase prepared in-house [20]), α-fucosidases (bovine kidney from Sigma-Aldrich or almond α1,3-specific from ProZyme), α-mannosidases …”

Product: α-fucosidases (almond α1,3-specific)

EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae

Shadnezhad, A; Naegeli, A; Sjögren, J; Adamczyk, B; Leo, F; Allhorn, M; Karlsson, NG; Jensen, A; Collin, M; Future Microbiol; 2016; 11: 721-36.

Snippet: “… The obtained chromatograms were compared with previously published and assigned IgG glycoforms [35,36]. The sequence, composition and linkage specificities of the glycans were analyzed by exoglycosidase digestion arrays (ProZyme, California). …”

Product: exoglycosidase digestion arrays

Candida albicans β-1,2-mannosyltransferase Bmt3 prompts the elongation of the cell-wall phosphopeptidomannan

Sfihi-Loualia, G; Hurtaux, T; Fabre, E; Fradin, C; Mée, A; Pourcelot, M; Maes, E; Bouckaert, J; Mallet, JM; Poulain, D; Delplace, F; Guérardel, Y; Glycobiology; 2016; 26(2): 203-14.

Snippet: “… The α-mannosidase treatment of the reaction mixture after mannosyltransferase assays was carried out in 20 mM sodium acetate buffer, pH 5, containing 2 mM ZnCl 2 and 0.15 units of α-exomannosidase from Jack Bean (ProZyme) at 37°C for 24–48 h. After centrifugation, the …”

Product: α-exomannosidase

O-Glycan analysis of cellobiohydrolase I from Neurospora crassa

Tang, SL; Bubner, P; Bauer, S; Somerville, CR; Glycobiology; 2016; 26(6): 670-7.

Snippet: “… PNGase F and exoglycosidases were purchased from New England Biolabs and ProZyme, Inc. For digestion with the β-1,4-mannosidase, 0.32 units of the enzyme (ProZyme) were added to 10 μg of trypsinated CBHI in 100 μL of 1× reaction buffer (ProZyme). …”

Product: β-1,4-mannosidase, trypsinated CBHI

From individual proteins to proteomic samples: characterization of O-glycosylation sites in human chorionic gonadotropin and human-plasma proteins

Bai, X; Li, D; Zhu, J; Guan, Y; Zhang, Q; Chi, L; Anal Bioanal Chem; 2015; 407(7): 1857-69.

Snippet: “…β(1-3,4) galactosidase, the GlycoPro Enzymatic Deglycosylation Kit, and prO-LINK Extender Kit containing PNGase F, β-N-acetylglucosaminidase, sialidase A, and standard glycoprotein bovine fetuin were purchased from ProZyme, California …”

Product: β(1-3,4) galactosidase, the GlycoPro Enzymatic Deglycosylation Kit, prO-LINK Extender Kit

Sweet secrets of a therapeutic worm: mass-spectrometric N-glycomic analysis of Trichuris suis

Wilson, IB; Paschinger, K; Anal Bioanal Chem; 2016; 408(2): 461-71.

Snippet: “… subject to treatment overnight, prior to MALDI-TOF MS, with either α-mannosidases (jack bean [30] from Sigma or Xanthomonas α1,2/3- or α1,6-specific [31] from New England Biolabs, Ipswich, MA), α-fucosidases (al- mond α1,3-fucosidase [32] from ProZyme, Hayward, California …”

Product: α-fucosidases (al- mond α1,3-fucosidase)

Multi-Angle Effector Function Analysis of Human Monoclonal IgG Glycovariants

Dashivets, T; Thomann, M; Rueger, P; Knaupp, A; Buchner, J; Schlothauer, T; PLoS ONE; 2015; 10(12): e0143520.

Snippet: “…To obtain the G1+1SA variant, 100 mg of the G2+1SA IgG1 (c = 4.75 mg/ml) were treated with 2 ml of the β(1–4)-Galactosidase (ProZyme, GKX-5014) to cleave the galactose from the non sialylated branch of the glycan. The sample was incubated at 37°C for 48 h To generate M3 glycovariant 5mg of G0 were treated with 1250 μl of β-N-acetylhexosaminidase (GKX-5003, ProZyme, 5U/100μl) at 37°C for 24 h...”

Product: β(1–4)-Galactosidase, β-N-acetylhexosaminidase

In vitro glycoengineering of IgG1 and its effect on Fc receptor binding and ADCC activity

Thomann, M; Schlothauer, T; Dashivets, T; Malik, S; Avenal, C; Bulau, P; Rüger, P; Reusch, D; PLoS ONE; 2015; 10(8): e0134949.

Snippet: “… Hypo-galactosylated variant. For degalactosylation, 750 μl β(1–4)-Galactosidase (ProZyme, GKX-5014) was added to 150 mg IgG1 (10 mU enzyme/mg antibody) and incubated at 37°C for 24 hours. The IgG1 was then purified via Protein A chromatography. …”

Product: β(1–4)-Galactosidase

Novel lectin-independent approach to detect galactose-deficient IgA1 in IgA nephropathy

Yasutake, J; Suzuki, Y; Suzuki, H; Hiura, N; Yanagawa, H; Makita, Y; Kaneko, E; Tomino, Y; Nephrol. Dial. Transplant.; 2015; 30(8): 1315-21.

Snippet: “… Commercially available human plasma IgA1 (BioPur AG, Switzerland) was incubated with β-galactosidase from bovine testes (ProZyme, California) and neuraminidase (Nacalai tesque, Kyoto, Japan) for 3 h at 37°C in sodium acetate buffer (pH5.0). …”

Product: β-galactosidase from bovine testes

Anti-EBOV GP IgGs Lacking α1-3-Galactose and Neu5Gc Prolong Survival and Decrease Blood Viral Load in EBOV-Infected Guinea Pigs

Reynard, O; Jacquot, F; Evanno, G; Mai, HL; Salama, A; Martinet, B; Duvaux, O; Bach, JM; Conchon, S; Judor, JP; Perota, A; Lagutina, I; Duchi, R; Lazzari, G; Le Berre, L; Perreault, H; Lheriteau, E; Raoul, H; Volchkov, V; Galli, C; Soulillou, JP; PLoS ONE; 2016; 11(6): e0156775.

Snippet: “…The digestion mixture was separated by reverse-phase HPLC. The pooled glycopeptide fractions were further digested with β-galactosidase from bovine testes (ProZyme, Hayward, California). All MS analyses …”

Product: β-galactosidase from bovine testes

N-Glycosylation analysis of formalin fixed paraffin embedded samples by capillary electrophoresis

Donczo, B; Szigeti, M; Ostoros, G; Gacs, A; Tovari, J; Guttman, A; Electrophoresis; 2015;: .

Snippet: “… 1–5 kidney fucosidase (BKF), Jack bean galactosidase (JBG), and Jack Bean hexosaminidase (JBH) were from ProZyme, Hayward, California. The 8-aminopyrene- 1,3,6-trisulfonate (APTS) and the maltooligosaccharide ladder were from SCIEX (Brea, California). …”

Product: kidney fucosidase (BKF), Jack bean galactosidase (JBG), Jack Bean hexosaminidase (JBH)

A procedure for the analysis of site-specific and structure-specific fucosylation in Alpha-1-Antitrypsin

Yin, H; Zhu, J; Wu, J; Tan, Z; An, M; Zhou, S; Mechref, Y; Lubman, DM; Electrophoresis; 2016;: .

Snippet: “…25 mM sodium acetate solution (pH5.5) and incubated with 15 mU (3 µL) of non-specific sialidase and 75 mU (3 µL) of β(1-4,6) galactosidase (ProZyme, Hayward, California) at 37°C for 18 h to remove all sialic acid residues and …”

Product: β(1-4,6) galactosidase

Candida albicans β-1,2 mannosyl transferase Bmt3: Preparation and evaluation of a β (1,2), α (1,2)-tetramannosyl fluorescent substrate

Cattiaux, L; Mée, A; Pourcelot, M; Sfihi-Loualia, G; Hurtaux, T; Maes, E; Fradin, C; Sendid, B; Poulain, D; Fabre, E; Delplace, F; Guérardel, Y; Mallet, JM; Bioorg. Med. Chem.; 2016; 24(6): 1362-8.

Snippet: “… The α-mannosidases treatment of the collected and purified [DP4+1] reaction product was carried out in 20 mM sodium acetate buffer pH 5, containing 2 mM ZnCl 2 and 0.15 units of α-exomannosidase from Jack Bean (ProZyme) at 37 °C for 24 h…”

Product: α-exomannosidase from Jack Bean

Increased mannosylphosphorylation of N-glycans by heterologous expression of YlMPO1 in glyco-engineered Saccharomyces cerevisiae for mannose-6-phosphate modification

Gil, JY; Park, JN; Lee, KJ; Kang, JY; Kim, YH; Kim, S; Kim, SY; Kwon, O; Lim, YT; Kang, HA; Oh, DB; J. Biotechnol.; 2015; 206: 66-74.

Snippet: “… Mild acid hydrolysis was carried out in 0.5 M formic acid at 80 °C for 1 h. After drying, α(1,2)-mannosidase (ProZyme, Hayward, California) and/or calf intestinal alkaline phosphatase (Takara, Tokyo, Japan) were treated in 20 mM ammnonium acetate pH 5.0 at 37 °C for 16 h. 3. Results. …”

Product: α(1,2)-mannosidase

Preeclampsia transforms membrane N-glycome in human placenta

Robajac, D; Vanhooren, V; Masnikosa, R; Miković, Ž; Mandić, V; Libert, C; Nedić, O; Exp. Mol. Pathol.; 2016; 100(1): 26-30.

Snippet: “… The presence of fucose was confirmed after digestion of APTS-labelled desialylated N-glycans using bovine kidney α-L-fucosidase (ProZyme, California) and DSA-FACE analysis. 2.3. Electrophoresis, lectin and immunoblotting. …”

Product: bovine kidney α-L-fucosidase

'Click chemistry' synthesis of 1-(α-D-mannopyranosyl)-1,2,3-triazoles for inhibition of α-mannosidases

Poláková, M; Stanton, R; Wilson, IB; Holková, I; Šesták, S; Machová, E; Jandová, Z; Kóňa, J; Carbohydr. Res.; 2015; 406: 34-40.

Snippet: “…Aspergillus saitoi α1,2-mannosidase was purchased from ProZyme and swainsonine and mannostatin A from Calbiochem...”

Product: Aspergillus saitoi α1,2-mannosidase

Sequential processing of mannose-containing glycans by two α-mannosidases from Solitalea canadensis

Liu, FF; Kulinich, A; Du, YM; Liu, L; Voglmeir, J; Glycoconj. J.; 2016; 33(2): 159-68.

Snippet: “… Reaction mixtures, containing commercial α-mannosidase from jack bean (ProZyme, Hayward), were used as positive controls. Samples were incubated for 16 h at 37 °C and acetonitrile was added for a final concentration of 80 % (v/v) prior to injection. …”

Product: α-mannosidase from jack bean

Characterization of N-glycosylation and amino acid sequence features of immunoglobulins from swine

Lopez, PG; Girard, L; Buist, M; de Oliveira, AG; Bodnar, E; Salama, A; Soulillou, JP; Perreault, H; Glycoconj. J.; 2016; 33(1): 79-91.

Snippet: “…Beta-galactosidase from bovine testis was obtained from ProZyme, Hayward, California. Ammonium water. Then, 5X buffer provided by the manufacturer (ProZyme, Hayward, California) and beta-galactosidase solution (0.5 U/mL) were added…”

Product: Beta-galactosidase from bovine testis

A solid-phase screening method for identification of glycan-binding cells

Kim, YW; Kim, KJ; Hwang, CH; Kim, YG; Biotechnology and Bioprocess Engineering; 2015; 20(2): 366–372.

Snippet: “…Half of the pig N-glycans were treated with the green coffee bean α-galactosidase (ProZyme, California) for the non-Gal epitopes…”

Product: green coffee bean α-galactosidase

Involvement of soil bacteria in ABO blood mistyping

Takada, N; Mori, C; Takai, R; Takayama, T; Watanabe, Y; Nakamura, K; Takamizawa, K; Leg Med (Tokyo); 2015; 17(2): 128-33.

Snippet: “… α-Galactosidase derived from green coffee bean was obtained from ProZyme, Hayward, California. Polyacrylamide-conjugated blood …”

Product: α-Galactosidase derived from green coffee bean

Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans

Garcia-Campos, A; Ravidà, A; Nguyen, DL; Cwiklinski, K; Dalton, JP; Hokke, CH; O'Neill, S; Mulcahy, G; PLoS Negl Trop Dis; 2016; 10(5): e0004688.

Snippet: “… glycans (1 μl/treatment) were incubated in a volume of 10 μl for 24 h at 37°C in 250 mM sodium citrate buffer with one of the following exoglycosidases: α-mannosidase from jack bean (15 μU/μl; Sigma Aldrich), β-galactosidase from jack bean (5 μU/μl; ProZyme)…”

Product: β-galactosidase from jack bean

Fasciola hepatica Surface Coat Glycoproteins Contain Mannosylated and Phosphorylated N-glycans and Exhibit Immune Modulatory Properties Independent of the Mannose Receptor

Ravidà, A; Aldridge, AM; Driessen, NN; Heus, FA; Hokke, CH; O'Neill, SM; PLoS Negl Trop Dis; 2016; 10(4): e0004601.

Snippet: “… 2-AA-labeled-N-glycans were incubated with Jack bean α-mannosidase, neuraminidase from Vibrio cholera, β-N-acetyl glucosaminidase from Canavalia ensiformis (all from Sigma-Aldrich, Wicklow, Ireland), or Jack bean β-(1–4,6) galactosidase (ProZyme, Hayward, California)…”

Product: Jack bean β-(1–4,6) galactosidase

Production of a camelid antibody in CHO cells and its potential for the in vitro modification of its N-glycan using Nacetylglucosaminyltransferase III produced in insect cells

Ang, JC; Thesis; 2015;: .

Snippet: “…Purified EG2 mAb underwent a buffer exchange into 50 mM sodium phosphate, pH 6.0 using centrifugal ultrafiltration (10 kDa cutoff, Millipore, Massachusetts). The samples were reacted with Streptococcus pneumonia ß-1,4 galactosidase (ProZyme, California) and sialidase (ProZyme, California) at 37 °C for 24 h with 1:500 ratio for enzyme:EG2 sample...”

Product: Streptococcus pneumonia ß-1,4 galactosidase, sialidase

An integrated approach to analyze EG2-hFc monoclonal antibody N-glycosylation by MALDI-MS

Bodnar, E; Ferreira Nascimento, T; Girard, L; Komatsu, E; Lopez, P; Gomes de Oliveira, AG; Roy, R; Smythe, T; Zogbi, Y; Spearman, M; Tay, VS; Butler, M; Perreault, H; Canadian Journal of Chemistry; 2015; 93(7): 754-763.

Snippet: “… Beta-galactosidase from bovine testis was obtained from ProZyme, Hayward, California. Pooled glycopeptide fractions were dried and resuspended in water. Then, 5× buffer provided by the manufacturer (ProZyme) and beta-galactosidase solution were added. …”

Product: Beta-galactosidase

Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology

Laukens, B; De Wachter, C; Callewaert, N; Methods Mol. Biol.; 2015; 1321: 103-22.

Snippet: “…Exoglycosidases: Jack Bean α-mannosidase, 20 mU per digestion; Trichoderma reesei α-1,2-mannosidase (Available from our laboratory), 0.33 μg per digestion; β-N- acetylhexosaminidase from Jack Bean (ProZyme, Hayward, California), 10 mU per digestion…”

Product: β-N- acetylhexosaminidase from Jack Bean

Identification of Mammalian O-Mannosylated Glycopeptides

Winterhalter, PR; Ruppert, T; Strahl, S; Glycoscience: Biology and Medicine; 2015;: .

Snippet: “… Take 20 μl of the enriched O-mannosylated glycopeptides. Add 5 μl 5× mannosidase buffer and 1 μl α-(1-2,3,6)-mannosidase (ProZyme, GKX-5010), mix, and incubate at 37 °C on an Eppendorf Thermomixer at 300 rpm for 16 h …”

Product: α-(1-2,3,6)-mannosidase

Towards personalized diagnostics via longitudinal study of the human plasma N-glycome

Hennig, R; Cajic, S; Borowiak, M; Hoffmann, M; Kottler, R; Reichl, U; Rapp, E; Biochim. Biophys. Acta; 2016; 1860(8): 1728-38.

Snippet: “…Exoglycosidases α(2-3) sialidase (Sialidase S, GK80020), α(2-3,6,8) sialidase (Sialidase A, GK80040), α(1-2,3,4,6) fucosidase (GKX-5006) and α(1-2,3,6) mannosidase (GKX-5010) were purchased from ProZyme, California. …”

Product: Exoglycosidases α(2-3) sialidase (Sialidase S, GK80020), α(2-3,6,8) sialidase (Sialidase A, GK80040), α(1-2,3,4,6) fucosidase (GKX-5006) and α(1-2,3,6) mannosidase (GKX-5010)

Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags

Kang, JY; Kwon, O; Gil, JY; Oh, DB; Data Brief; 2016; 7: 1531-7.

Snippet: “… Their identities were further confirmed by the successive digestions of α(1,2)-mannosidase (ProZyme, Haywoard, California) and calf intestinal alkaline phosphatase (Takara, Tokyo, Japan) as previously described [3]. The α(1,2)-mannosidase treatment converted high-mannose type …”

Product: α(1,2)-mannosidase