Citations - N-Glycanase (PNGase F)

Lypd8 promotes the segregation of flagellated microbiota and colonic epithelia

Okumura, R; Kurakawa, T; Nakano, T; Kayama, H; Kinoshita, M; Motooka, D; Gotoh, K; Kimura, T; Kamiyama, N; Kusu, T; Ueda, Y; Wu, H; Iijima, H; Barman, S; Osawa, H; Matsuno, H; Nishimura, J; Ohba, Y; Nakamura, S; Iida, T; Yamamoto, M; Umemoto, E; Sano, K; Takeda, K; Nature; 2016; 532(7597): 117-21.

Snippet: “…Recombinant Lypd8 proteins (1μg) were incubated with PNGase F, sialidase A and O-glycanase (ProZyme) at 37°C for 3 h…”

Product: PNGase F, sialidase A, O-glycanase

Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase Activity Independent of Nuclease Activity to Suppress Immune Activation

Hasan, M; Fermaintt, CS; Gao, N; Sakai, T; Miyazaki, T; Jiang, S; Li, QZ; Atkinson, JP; Morse, HC; Lehrman, MA; Yan, N; Immunity; 2015; 43(3): 463-74.

Snippet: “…The glycan units of LLOs were released with weak acid, the N-glycans were released with peptide:N-glycosidase F (C. meningosepticum, ProZyme) or Endoglycosidase H (S. picatus, New England Biolabs), the O-glycans were released with base and sialic acids removed with weak acid, and all three glycan fractions were further purified with ion-exchange resins...”

Product: N-glycosidase F (C. meningosepticum)

Molecular Basis of Assembly and Activation of Complement Component C1 in Complex with Immunoglobulin G1 and Antigen

Wang, G; de Jong, RN; van den Bremer, ET; Beurskens, FJ; Labrijn, AF; Ugurlar, D; Gros, P; Schuurman, J; Parren, PW; Heck, AJ; Mol. Cell; 2016; 63(1): 135-45.

Snippet: “…Deglycosylation of IgG for complement assays was performed by incubation with PNGase F (ProZyme) at 37°C for at least 24 hr...”

Product: PNGase F

Glycosylation Profile of IgG in Moderate Kidney Dysfunction

Barrios, C; Zierer, J; Gudelj, I; Štambuk, J; Ugrina, I; Rodríguez, E; Soler, MJ; Pavić, T; Šimurina, M; Keser, T; Pučić-Baković, M; Mangino, M; Pascual, J; Spector, TD; Lauc, G; Menni, C; J. Am. Soc. Nephrol.; 2016; 27(3): 933-41.

Snippet: “… 42. Glycan Release and Labeling. Glycan release and labeling were performed essentially as previously described. 24,42 Briefly, dried IgG was denatured with 2% SDS (wt/vol) and N-glycans were released by digestion with PNGase F (ProZyme, Hayward, California). …”

Product: PNGase F

N-glycomic profiling as a tool to separate rectal adenomas from carcinomas

Kaprio, T; Satomaa, T; Heiskanen, A; Hokke, CH; Deelder, AM; Mustonen, H; Hagström, J; Carpen, O; Saarinen, J; Haglund, C; Mol. Cell Proteomics; 2015; 14(2): 277-88.

Snippet: “…Glycans were detached from cellular glycoproteins by PNGase F digestion (ProZyme, Hayward , California)...”

Product: PNGase F

N-linked (N-) glycoproteomics of urinary exosomes

Saraswat, M; Joenväära, S; Musante, L; Peltoniemi, H; Holthofer, H; Renkonen, R; Molecular & Cellular Proteomics; 2015; 14(2): 263-276.

Snippet: “… format. Glycomics. N-glycan analysis was performed to 250 μl of samples 1–5. Asparagine-linked glycans were detached from the proteins by Elizabethkingia meningoseptica N-glycosidase F digestion (ProZyme, Hayward , California). …”

Product: Elizabethkingia meningoseptica N-glycosidase F digestion

The metacaspase Mca1p restricts O-glycosylation during farnesol-induced apoptosis in Candida albicans

Léger, T; Garcia, C; Camadro, JM; Mol. Cell Proteomics; 2016;: .

Snippet: “… 100 µl of 20 mM triethylammonium bicarbonate (TEAB) buffer overnight at 37°C. We added 5 µl of the glycosidase PNGase F (ProZyme) and the samples were incubated for 16 hours at 37°C with continuous shaking. A conditioned …”

Product: PNGase F

Presenilin 1 Maintains Lysosomal Ca(2+) Homeostasis via TRPML1 by Regulating vATPase-Mediated Lysosome Acidification

Lee, JH; McBrayer, MK; Wolfe, DM; Haslett, LJ; Kumar, A; Sato, Y; Lie, PP; Mohan, P; Coffey, EE; Kompella, U; Mitchell, CH; Lloyd-Evans, E; Nixon, RA; Cell Rep; 2015; 12(9): 1430-44.

Snippet: “…To assess FLAG-tagged V0a1 mutant glycosylation, samples were treated for 24 hr at 37°C with PNGase F using an enzymatic deglycosylation kit according to the manufacturer’s instruction (ProZyme)...”

Product: PNGase F

Computationally Designed Bispecific Antibodies using Negative State Repertoires

Leaver-Fay, A; Froning, KJ; Atwell, S; Aldaz, H; Pustilnik, A; Lu, F; Huang, F; Yuan, R; Hassanali, S; Chamberlain, AK; Fitchett, JR; Demarest, SJ; Kuhlman, B; Structure; 2016; 24(4): 641-51.

Snippet: “…Proteins were deglycosylated by the addition of 1 μl of N-glycanase (ProZyme) for 3–14 hr at 37°C prior to being submitted for LCMS...”

Product: PNGase F (N-Glycanase)

Glycosylation of rhodopsin is necessary for its stability and incorporation into photoreceptor outer segment discs

Murray, AR; Vuong, L; Brobst, D; Fliesler, SJ; Peachey, NS; Gorbatyuk, MS; Naash, MI; Al-Ubaidi, MR; Hum. Mol. Genet.; 2015; 24(10): 2709-23.

Snippet: “…To deglycosylate retinal proteins, we followed the manufacturer's protocol for N-Glycanase (Peptide-N-Glycosidase F, PNGase F; ProZyme , California)...”

Product: PNGase F

A mouse model of a human congenital disorder of glycosylation caused by loss of PMM2

Chan, B; Clasquin, M; Smolen, GA; Histen, G; Powe, J; Chen, Y; Lin, Z; Lu, C; Liu, Y; Cang, Y; Yan, Z; Xia, Y; Thompson, R; Singleton, C; Dorsch, M; Silverman, L; Su, SM; Freeze, HH; Jin, S; Hum. Mol. Genet.; 2016;: .

Snippet: “…Control BL/6 mouse plasma was either untreated (intact glycosylation) or deglycosylated with N-Glycanase (ProZyme) per the manufacturer’s protocol...”

Product: PNGase F (N-Glycanase)

An O-Glycosylation of Fibronectin Mediates Hepatic Osteodystrophy Through α4β1 Integrin

Sens, C; Altrock, E; Rau, K; Klemis, V; von Au, A; Pettera, S; Uebel, S; Damm, T; Tiwari, S; Moser, M; Nakchbandi, IA; J. Bone Miner. Res.; 2016;: .

Snippet: “… - Deglycosylation experiments Deglycosylation of fibronectin isoforms was performed using N-glycanase (PNGase F), sialidase A, O- glycanase (all from ProZyme). Briefly, for N-deglycosylation, 100 µg oFN were treated with 4 mU PNGase F overnight. …”

Product: N-glycanase (PNGase F), sialidase A, O- glycanase

Effect of Separation Temperature on Structure Specific Glycan Migration in Capillary Electrophoresis

Guttman, A; Kerekgyarto, M; Jarvas, G; Anal. Chem.; 2015; 87(23): 11630-4.

Snippet: “… Louis, MO). PNGase F was from ProZyme, Hayward, California. The linear polyacrylamide solution (50%, 10 kDa) was from Polysciences (Warrington, PA). High purity 8-aminopyrene-1,3,6- trisulfonic acid (APTS) and the maltooligosaccharide ladder were from SCIEX (Brea, California). …”

Product: PNGase F

Identification of glycoproteins containing specific glycans using a lectin-chemical method

Li, Y; Shah, P; De Marzo, AM; Van Eyk, JE; Li, Q; Chan, DW; Zhang, H; Anal. Chem.; 2015; 87(9): 4683-7.

Snippet: “… Sequencing-grade trypsin was from Promega (Madison, WI). Peptide-N-glycosidase F (PNGase) was from ProZyme, California). Sodium periodate, hydrazide resin, and P6 desalting spin columns were from Bio-Rad (Hercules, California). …”

Product: Peptide-N-glycosidase F (PNGase)

Assignment of Core versus Antenna Fucosylation Types in Protein N-Glycosylation via Procainamide Labeling and Tandem Mass Spectrometry

Nwosu, C; Yau, HK; Becht, S; Anal. Chem.; 2015; 87(12): 5905-13.

Snippet: “…Purified water was obtained from an in-house Milli-Q system. HPLC grade acetonitrile (ACN) was used. PNGase F, Human IgG, and the solid phase extraction (SPE) manifold were purchased from ProZyme (Hayward, California). …”

Product: PNGase F, Human IgG, solid phase extraction (SPE) manifold

Detection and quantitation of low abundance oligosaccharides in recombinant monoclonal antibodies

Ponniah, G; Nowak, C; Gonzalez, N; Miano, D; Liu, H; Anal. Chem.; 2015; 87(5): 2718-26.

Snippet: “…PNGase F, endoglycosidase H (Endo H), β-galactosidase, β-N-acetylhexosaminidase, mannosidase, and fucosidase were purchased from ProZyme, Hayward, California. Residual reagents were removed using GlycoClean S cartridges (ProZyme). …”

Product: PNGase F, endoglycosidase H (Endo H), β-galactosidase, β-N-acetylhexosaminidase, mannosidase, fucosidase, GlycoClean S cartridges

Identification of low abundant isomeric N-glycan structures in biological therapeutics by LC/MS

Zhao, J; Li, S; Li, C; Wu, SL; Xu, W; Chen, Y; Shameem, M; Richardson, DD; Li, H; Anal. Chem.; 2016;: .

Snippet: “… Louis, MO). N Glycanase (PNGase F) was purchased from ProZyme, Hayward, California and New England Biolab, (Lpswich, MA). SignalTM 2-AB labeling kit and GlycoCleanTM S Cartridges were purchased from ProZyme, Hayward, California. Release of glycans by PNGaseF …”

Product: N Glycanase (PNGase F), SignalTM 2-AB labeling kit, GlycoCleanTM S Cartridges

Inflammatory bowel disease associates with proinflammatory potential of the immunoglobulin G glycome

Akmacic, IT; Ventham, NT; Theodoratou, E; Vučković, F; Kennedy, NA; Krištić, J; Nimmo, ER; Kalla, R; Drummond, H; Štambuk, J; Dunlop, MG; Novokmet, M; Aulchenko, Y; Gornik, O; Campbell, H; Pučić Baković M; Satsangi, J; Lauc, G; IBD-BIOM Consortium. Inflammatory Bowel Diseases; 2015; 21(6): 1237–1247.

Snippet: “…Subsequently, 10 mL of 4% Igepal-CA630 (vol/vol) (Sigma-Aldrich, St. Louis, MO) and 1.25 mU of PNGase F (ProZyme, Hayward, California) in 10 mL 5· PBS were added. T…”

Product: PNGase F

The Association Between Low Back Pain and Composition of IgG Glycome

Freidin, MB; Keser, T; Gudelj, I; Štambuk, J; Vučenović, D; Allegri, M; Pavić, T; Šimurina, M; Fabiane, SM; Lauc, G; Williams, FM; Sci Rep; 2016; 6: 26815.

Snippet: “… Subsequently, 10 μL of 4% Igepal-CA630 (Sigma-Aldrich, St. Louis, MO) and 1.25 mU of PNGase F (ProZyme) in 10 μL 5 × phosphate-buffered saline were added to the samples. The samples were incubated overnight at 37 °C for N-glycan release. …”

Product: PNGase F

DNA hypomethylation upregulates expression of the MGAT3 gene in HepG2 cells and leads to changes in N-glycosylation of secreted glycoproteins

Klasić, M; Krištić, J; Korać, P; Horvat, T; Markulin, D; Vojta, A; Reiding, KR; Wuhrer, M; Lauc, G; Zoldoš, V; Sci Rep; 2016; 6: 24363.

Snippet: “…Briefly, N-glycans were released by PNGase F (ProZyme), labelled with 2-aminobenzimidine (2-AB) fluorescent dye (Sigma-Aldrich) and purified by HILIC-SPE to remove excess free label. HILIC-UPLC. Fluorescently labelled …”

Product: PNGase F

Glycosylation of plasma IgG in colorectal cancer prognosis

Theodoratou, E; Thaçi, K; Agakov, F; Timofeeva, MN; Štambuk, J; Pučić-Baković, M; Vučković, F; Orchard, P; Agakova, A; Din, FV; Brown, E; Rudd, PM; Farrington, SM; Dunlop, MG; Campbell, H; Lauc, G; Sci Rep; 2016; 6: 28098.

Snippet: “…1.25 mU of PNGase F (ProZyme, Hayward, California) in 10 μL 5× PBS were added to the samples and incubated overnight at 37 °C to release N-glyans. The released N-glycans were labelled with 2-aminobenzamide (2-AB). …”

Product: PNGase F

Serum N-Glycome Characterization in Patients with Resectable Periampullary Adenocarcinoma

Hamfjord, J; Saldova, R; Stöckmann, H; Sandhu, V; Bowitz Lothe, IM; Buanes, T; Lingjærde, OC; Labori, KJ; Rudd, PM; Kure, EH; J. Proteome Res.; 2015; 14(12): 5144-56.

Snippet: “…Briefly, the samples were denaturated with dithiothreitol, alkylated with iodoacetamide, and N-glycans were released from the protein backbone enzymatically via PNGase F (ProZyme Glyco NGlycanase, code GKE-5006D, 10 μL per well, 0.5 mU in 1 M ammonium bicarbonate, pH 8.0)...”

Product: PNGase F

Efficient Procedure for N-Glycan Analyses and Detection of Endo H-Like Activity in Human Tumor Specimens

Lattová, E; Bryant, J; Skřičková, J; Zdráhal, Z; Popovič, M; J. Proteome Res.; 2016;: .

Snippet: “…After cooling detergent solution (2.5 μL) was added, mixed with PNGase F (1–3 mU, ProZyme or Roche) and incubated at 37 °C for 1–12 h…”

Product: PNGase F

Mass spectrometric quantification of N-linked glycans by reference to exogenous standards

Mehta, N; Porterfield, M; Struwe, WB; Heiss, C; Azadi, P; Rudd, PM; Tiemeyer, M; Aoki, K; J. Proteome Res.; 2016;: .

Snippet: “… PNGaseF (N-glycanase) was obtained from ProZyme, California. N-glycan standards were obtained from the Consortium for Functional Glycomics (CFG) and from The Scripps Research Institute …”

Product: PNGaseF(N-glycanase)

The Association Between Glycosylation of Immunoglobulin G and Hypertension: A Multiple Ethnic Cross-Sectional Study

Wang, Y; Klarić, L; Yu, X; Thaqi, K; Dong, J; Novokmet, M; Wilson, J; Polasek, O; Liu, Y; Krištić, J; Ge, S; Pučić-Baković, M; Wu, L; Zhou, Y; Ugrina, I; Song, M; Zhang, J; Guo, X; Zeng, Q; Rudan, I; Campbell, H; Aulchenko, Y; Lauc, G; Wang, W; Medicine (Baltimore); 2016; 95(17): e3379.

Snippet: “…Glycan release and labeling of Vis and Korčula samples were performed by the “in gel” method as reported by Royle et al.39 Briefly, IgG was immobilized in a block of sodium dodecyl sulfate-polyacrylamide gel in a 96-well microtiter plate, and N-glycans were released by digestion with recombinant N-glycosidase F (ProZyme, California). …”

Product: recombinant N-glycosidase F

Circular trimers of gelatinase B/matrix metalloproteinase-9 constitute a distinct population of functional enzyme molecules differentially regulated by tissue inhibitor of metalloproteinases-1

Vandooren, J; Born, B; Solomonov, I; Zajac, E; Saldova, R; Senske, M; Ugarte-Berzal, E; Martens, E; Van den Steen, PE; Van Damme, J; Garcia-Pardo, A; Froeyen, M; Deryugina, EI; Quigley, JP; Moestrup, SK; Rudd, PM; Sagi, I; Opdenakker, G; Biochem. J.; 2015; 465(2): 259-70.

Snippet: “…N-glycans were released from gelatinase B samples by in situ digestion with N-glycosidase F (PNGase F, ProZyme) in-gel-blocks as previously described [23]. …”

Product: PNGase F

Protein design of IgG/TCR chimeras for the co-expression of Fab-like moieties within bispecific antibodies

Wu, X; Sereno, AJ; Huang, F; Zhang, K; Batt, M; Fitchett, JR; He, D; Rick, HL; Conner, EM; Demarest, SJ; MAbs; 2015; 7(2): 364-76.

Snippet: “…Thirty mL aliquots of the proteins were enzymatically deglycosylated after purification and neutralization by the addition of 1 mL N-Glycanase (ProZyme) for 3–14 hours at 37C prior to being submitted for LCMS...”

Product: PNGase F (N-Glycanase)

Comparing domain interactions within antibody Fabs with kappa and lambda light chains

Toughiri, R; Wu, X; Ruiz, D; Huang, F; Crissman, JW; Dickey, M; Froning, K; Conner, EM; Cujec, TP; Demarest, SJ; MAbs; 2016;: 0.

Snippet: “…Imidazole, PH 8.0. All proteins were concentrated, dialyzed against PBS, aliquoted, and stored at 4 °C until characterized. Enzymatic deglycosylation of the Ca/Cb domains within the Ca/Cb- containing pertuzumab Fab was performed using N-Glycanase® (ProZyme) according to the manufacturer’s instructions...”

Product: PNGase F (N-Glycanase)

Structural Aspects of N-Glycosylations and the C-terminal Region in Human Glypican-1

Awad, W; Adamczyk, B; Örnros, J; Karlsson, NG; Mani, K; Logan, DT; J. Biol. Chem.; 2015; 290(38): 22991-3008.

Snippet: “… N-Glycans were enzymatically released by digestion with recombinant N-glycosidase F (50 μl, 0.5 milliunits in 20 mm NaHCO 3, pH 7.0) (ProZyme, Hayward, California) and incubated at 37 °C overnight…”

Product: N-glycosidase F

Recognition of N-glycoforms in human chorionic gonadotropin by monoclonal antibodies and their interaction motifs

Li, D; Zhang, P; Li, F; Chi, L; Zhu, D; Zhang, Q; Chi, L; J. Biol. Chem.; 2015; 290(37): 22715-23.

Snippet: “… The recombinant hCG β subunit expressed in Pichia pastoris was purchased from Sigma-Aldrich. Peptide-N-glycosidase F (PNGase F) was from ProZyme, California. Pepsin from porcine gastric mucosa and Trypsin Gold were purchased from Promega (Madison, WI). …”

Product: Peptide-N-glycosidase F (PNGase F)

Kin of IRRE-like Protein 2 Is a Phosphorylated Glycoprotein That Regulates Basal Insulin Secretion

Yesildag, B; Bock, T; Herrmanns, K; Wollscheid, B; Stoffel, M; J. Biol. Chem.; 2015; 290(43): 25891-906.

Snippet: “… For N-linked glycan removal, lysates of MIN6 cells were treated with the indicated units of N-glycanase enzyme (ProZyme) following the manufacturer's instructions for 1 h at 37 °C, and the reactions were stopped by addition of Laemmli buffer with DTT. …”

Product: N-glycanase enzyme

N-linked glycosylation of Kv1.2 voltage-gated potassium channel facilitates cell surface expression and enhances the stability of internalized channels

Thayer, DA; Yang, SB; Jan, YN; Jan, LY; J. Physiol. (Lond.); 2016;: .

Snippet: “… The glycosidases PNGase F, Endo H, sialidase T, and sialidase V (ProZyme) and fetuin (Sigma) were also used. Glycosidase reactions were performed on-resin according to ProZyme's recommended protocol with supplied buffers at 37oC for 4 hours. …”

Product: glycosidases PNGase F, Endo H, sialidase T, sialidase V

Automated, high-throughput serum glycoprofiling platform

Stöckmann, H; O'Flaherty, R; Adamczyk, B; Saldova, R; Rudd, PM; Integr Biol (Camb); 2015; 7(9): 1026-32.

Snippet: “… PNGase F (ProZyme N-Glycanase, code GKE-5006D, 10 μL per well, 0.5 mU in 1 M ammonium bicarbonate, pH 8.0) was added and the plate was insulated with an anti-evaporation lid and incubated at 40 °C with agitation at 700 rpm for 120 min. …”

Product: PNGase F

Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture

Hu, D; Sun, Y; Liu, X; Liu, J; Zhang, X; Zhao, L; Wang, H; Tan, WS; Fan, L; Appl. Microbiol. Biotechnol.; 2015; 99(20): 8429-40.

Snippet: “… The N-linked glycans of Fc-fusion protein were analyzed using the NP-HPLC method (Royle et al. 2008). In short, N-linked glycans were released from purified Fc-fusion protein by overnight incubation with peptide-N-glycosidase F (ProZyme, California). …”

Product: peptide-N-glycosidase F

Proteomics of the human endometrial glandular epithelium and stroma from the proliferative and secretory phases of the menstrual cycle

Hood, BL; Liu, B; Alkhas, A; Shoji, Y; Challa, R; Wang, G; Ferguson, S; Oliver, J; Mitchell, D; Bateman, NW; Zahn, CM; Hamilton, CA; Payson, M; Lessey, B; Fazleabas, AT; Maxwell, GL; Conrads, TP; Risinger, JI; Biol. Reprod.; 2015; 92(4): 106.

Snippet: “... Protein samples were quantified by the Bradford assay and subjected to immunoblotting as described below. N-glycanase (ProZyme) treatments were performed as previously described [28]. Immunoblotting. Ten micrograms …”

Product: PNGase F (N-Glycanase)

Optimization of influenza A vaccine virus by reverse genetic using chimeric HA and NA genes with an extended PR8 backbone

Medina, J; Boukhebza, H; De Saint Jean, A; Sodoyer, R; Legastelois, I; Moste, C; Vaccine; 2015; 33(35): 4221-7.

Snippet: “… Fixed amounts of purified viruses were deglycosylated by using PNGase F from Elizabethkingia miricola enzyme (P7367, Sigma Aldrich, Saint-Louis) and buffers from the ProZyme N-Glycanase kit (GKE-5006B ProZyme, Hayward). …”

Product: N-Glycanase kit (GKE-5006B)

Properties of neurons derived from induced pluripotent stem cells of Gaucher disease type 2 patient fibroblasts: potential role in neuropathology

Sun, Y; Florer, J; Mayhew, CN; Jia, Z; Zhao, Z; Xu, K; Ran, H; Liou, B; Zhang, W; Setchell, KD; Gu, J; Grabowski, GA; PLoS ONE; 2015; 10(3): e0118771.

Snippet: “… Protein concentration was determined by the BCA assay. For N-Glycanase (ProZyme, GKE-5006) reactions, proteins in the lysates were denatured at 100°C for 5 min in 0.1% SDS and 50mM β-mercaptoethanol. After …”

Product: PNGase F (N-Glycanase)

Designed Amino Acid Feed in Improvement of Production and Quality Targets of a Therapeutic Monoclonal Antibody

Torkashvand, F; Vaziri, B; Maleknia, S; Heydari, A; Vossoughi, M; Davami, F; Mahboudi, F; PLoS ONE; 2015; 10(10): e0140597.

Snippet: “… Glycoprofiling of IgG. Ig Gglycoprofiling was carried out using N-Glycanase Kit (ProZyme, California) for quantifying the total of each N-glycans. Digestion and labelling of N-glycans from IgG was performed according to the manufacturer's instructions. …”

Product: PNGase F (N-Glycanase)

Comprehensive N-Glycan Profiling of Avian Immunoglobulin Y.

Gilgunn, S; Millán Martín, S; Wormald, MR; Zapatero-Rodríguez, J; Conroy, PJ; O'Kennedy, RJ; Rudd, PM; Saldova, R; PLoS ONE; 2016; 11(7): e0159859.

Snippet: “… Glycan release. N-glycans were released from the dissolved samples (1 mg/mL) using PNGase F, (100 mU/mL; ProZyme, California) in accordance with methods previously described by Royle et al.…”

Product: PNGase F

Comparative glycoprofiling of HIV gp120 immunogens by capillary electrophoresis and MALDI mass spectrometry

Guttman, M; Váradi, C; Lee, KK; Guttman, A; Electrophoresis; 2015; 36(42686): 1305-13.

Snippet: “… PNGase F) endoglycosidase and the exoglycosidases of Arthrobacter ureafaciens sialidase (ABS), bovine kidney fucosidase (BKF), almond meal alpha-fucosidase (AMF), jack bean galactosidase (JBG), and jack bean hexosaminidase (JBH) were from ProZyme, Hayward, California …”

Product: PNGase F) endoglycosidase, exoglycosidases of Arthrobacter ureafaciens sialidase (ABS), bovine kidney fucosidase (BKF), almond meal alpha-fucosidase (AMF), jack bean galactosidase (JBG), jack bean hexosaminidase (JBH)

GUcal: An integrated application for capillary electrophoresis based glycan analysis

Jarvas, G; Szigeti, M; Guttman, A; Electrophoresis; 2015; 36(24): 3094-6.

Snippet: “… Human IgG was purchased from Sigma–Aldrich (St. Louis, MO). PNGase F enzyme was from ProZyme, Hayward, California. Details of the sample preparation protocol were published earlier in [1]. CE separations …”

Product: PNGase F

Expression and characterization of a novel recombinant version of the secreted human mucin MUC5AC in airway cell lines

Ryan, A; Smith, A; Moore, P; McNally, S; Carrington, SD; Reid, CJ; Clyne, M; Biochemistry; 2015; 54(4): 1089-99.

Snippet: “… PNGase F Digestion of Recombinant Protein PNGaseF (ProZyme, EC 3.5.1.52) was used to remove N-glycans from recombinant mucins. Purified recombinant MUC5AC protein was added …”

Product: PNGaseF

Combination of IgG N-glycomics and corresponding transcriptomics data to identify anti-TNF-α treatment responders in inflammatory diseases

Váradi, C; Holló, Z; Póliska, S; Nagy, L; Szekanecz, Z; Váncsa, A; Palatka, K; Guttman, A; Electrophoresis; 2015; 36(42686): 1330-5.

Snippet: “…The PNGase F enzyme and the GlycoPrep clean-up modules were from ProZyme, Hayward, California bicarbonate. For PNGase F digestion the ProZyme deglycosylation chemistry was used according to the manufacturer's protocol. …”

Product: PNGase F enzyme, GlycoPrep

Using lysine-reactive fluorescent dye for surface characterization of a mAb

Lei, M; Kao, YH; Schöneich, C; J Pharm Sci; 2015; 104(3): 995-1004.

Snippet: “… PNGase F was purchased from ProZyme, California. Trypsin, sequencing grade was acquired from Promega (Madison, Wisconsin), whereas Glu-C was obtained from Roche Diagnostics (Indianapolis, Indiana). …”

Product: PNGase F

ERAP1 reduces accumulation of aberrant and disulfide-linked forms of HLA-B27 on the cell surface

Tran, TM; Hong, S; Edwan, JH; Colbert, RA; Mol. Immunol.; 2016; 74: 7-Oct.

Snippet: “… Methyl methanethiosulfonate (MMTS) was obtained from ThermoScientific (Rockford, IL), and N-glycanase from ProZyme, Hayward, California. 2.2. Cell lines and ERAP1 knockdown. The human monocytic …”

Product: PNGase F (N-Glycanase)

Mass spectrometry based analysis of human plasma-derived factor X revealed novel post-translational modifications

Chevreux, G; Tilly, N; Faid, V; Bihoreau, N; Protein Sci.; 2015; 24(10): 1640-8.

Snippet: “… Trypsin, Asp-N and Glu-C were purchased from Roche (Mannheim, Germany). Peptide-N-glycosidase F (PNGase F) was from ProZyme. 2-amino-anthranilic acid (2-AA)-labeling kit was from Europa Bioproducts (Cambridge, England). …”

Product: Peptide-N-glycosidase F

High-density mammalian cell cultures in stirred-tank bioreactor without external pH control

Xu, S; Chen, H; J. Biotechnol.; 2016; 231: 149-59.

Snippet: “… Glycans were released from Protein A purified antibody using N-glycanase (ProZyme, Hayward, California), then labeled with Instant AB dye (ProZyme) and cleaned up on cleanup cartridges …”

Product: PNGase F (N-Glycanase)

Phosphoneurofilament heavy chain and N-glycomics from the cerebrospinal fluid in amyotrophic lateral sclerosis

Gonçalves, M; Tillack, L; de Carvalho, M; Pinto, S; Conradt, HS; Costa, J; Clin. Chim. Acta; 2015; 438: 342-9.

Snippet: “… Fractions were then neutralized with 2.5% NH3 . Glycopeptides were dried in the Speed Vac concentrator, solubilised in 50 mM Tris–HCl pH 7.0 and the N-glycans were released with 10 mU PNGase F (ProZyme), at 37 °C, overnight following the supplier's protocol. …”

Product: PNGase F

Separation window dependent multiple injection (SWDMI) for large scale analysis of therapeutic antibody N-glycans

Kovács, Z; Szarka, M; Szigeti, M; Guttman, A; Journal of Pharmaceutical and Biomedical Analysis; 2016; 128: 367-370.

Snippet: “… PNGase F was from ProZyme, Hayward, California. The 8-aminopyrene-1,3,6-trisulfonate (APTS) and NCHO Carbohydrate Separation Buffer were from SCIEX (Brea, California). …”

Product: PNGase F

Limited proteolysis and peptide mapping for comparability of biopharmaceuticals: An evaluation of repeatability, intra-assay precision and capability to detect structural change

Perrin, C; Burkitt, W; Perraud, X; O'Hara, J; Jone, C; J Pharm Biomed Anal; 2016; 123: 162-72.

Snippet: “… The deglycosylated sample was prepared by incubation of 2 mg of mAb1 at 1 mg mL −1 with 40 mU of PNGase F (N-Glycanase) from ProZyme, California, at 37 °C for 18 h with 100 rpm agitation on a New Brunswick Platform shaker. …”

Product: PNGase F (N-Glycanase)

Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells

Böhm, E; Seyfried, BK; Dockal, M; Graninger, M; Hasslacher, M; Neurath, M; Konetschny, C; Matthiessen, P; Mitterer, A; Scheiflinger, F; BMC Biotechnol.; 2015; 15: 87.

Snippet: “… All samples were desalted by acetone precipitation. For O-glycan monosaccharide composition, samples were digested with PNGase F (ProZyme, Hayward, California) at 37 °C overnight. Liberated N-glycans were removed by ethanol precipitation. …”

Product: PNGase F

A simple enzyme-substrate localized conjugation method to generate immobilized, functional glutathione S-transferase fusion protein columns for affinity enrichment

Coughlin, J; Masci, A; Gronke, RS; Bergelson, S; Co, C; Anal. Biochem.; 2016; 505: 51-8.

Snippet: “… Deglycosylated IgG1s were generated by incubation with Peptide-N- glycosidase F (PNGase F) from ProZyme (cat. no. GKE-5006A) according to the manufacturer's instructions. Deglycosylation was confirmed by mass spectrometry. …”

Product: Peptide-N- glycosidase F (PNGase F)

Conformational changes of recombinant monoclonal antibodies by limited proteolytic digestion, stable isotope labeling, and liquid chromatography-mass spectrometry

Ponniah, G; Nowak, C; Kita, A; Cheng, G; Kori, Y; Liu, H; Anal. Biochem.; 2016; 497: 7-Jan.

Snippet: “… Trypsin was purchased from Promega (Madison, WI). Chymotrypsin, Lys-C, and N-octylglucoside were purchased from Roche (Indianapolis, IN). PNGaseF was purchased from ProZyme, Hayward, California…”

Product: PNGaseF

Enhanced sialylation of a human chimeric IgG1 variant produced in human and rodent cell lines

Mimura, Y; Kelly, RM; Unwin, L; Albrecht, S; Jefferis, R; Goodall, M; Mizukami, Y; Mimura-Kimura, Y; Matsumoto, T; Ueoka, H; Rudd, PM; J. Immunol. Methods; 2016; 428: 30-6.

Snippet: “… The glycans were released from protein in the gel with peptide-N-glycosidase F (ProZyme) at 37 °C for 18 h, extracted from the gel pieces by repeated sonication steps in water, then acetonitrile, dried under vacuum and fluorescently labeled with 2-aminobenzamide (2-AB) dye. …”

Product: peptide-N-glycosidase F

Cell culture media supplementation of bioflavonoids for the targeted reduction of acidic species charge variants on recombinant therapeutic proteins

Hossler, P; Wang, M; McDermott, S; Racicot, C; Chemfe, K; Zhang, Y; Chumsae, C; Manuilov, A; Biotechnol. Prog.; 2015; 31(4): 1039-52.

Snippet: “… MS analysis. The collected samples were concentrated using centrifugal filter units (EMD Millipore, Billerica, MA) and subjected to PNGase F (ProZyme, Hayward, California) digestion for 16 h at 37°C to remove N-glycans…”

Product: PNGase F

Identifying a Robust Design Space for Glycosylation During Monoclonal Antibody Production

St Amand, MM; Hayes, J; Radhakrishnan, D; Fernandez, J; Meyer, B; Robinson, AS; Ogunnaike, BA; Biotechnol. Prog.; 2016.

Snippet: “… Phynexus, San Jose CA). Once purified, the IgG1 was cleaved with trypsin reconstituted in 1mM HCl (Promega, Madison WI) and glycans were cleaved using N-glycanase (ProZyme, Hayward CA). Cleaved glycans were purified …”

Product: PNGase F (N-Glycanase)

Faster in vivo clearance of human embryonic kidney than Chinese hamster ovary cell derived protein: Role of glycan mediated clearance

Wang, M; Ishino, T; Joyce, A; Tam, A; Duan, W; Lin, L; Somers, WS; Kriz, R; O'Hara, DM; J. Biosci. Bioeng.; 2015; 119(6): 657-60.

Snippet: “… Briefly, proteins were analyzed under reducing condition using Perkin Elmer LabChip GXII (Hopkinton, MA). Deglycosylated KLH-monoFc samples were prepared by incubating the protein with Glycannase F (ProZyme) for 3 h at 37°C in PBS buffer. …”

Product: PNGase F

Improving lactate metabolism in an intensified CHO culture process: productivity and product quality considerations

Xu, S; Hoshan, L; Chen, H; Bioprocess Biosyst Eng; 2016;: .

Snippet: “… Glycans were released from Protein A purified antibody using N-glycanase (ProZyme, Hayward, California), then labeled with Instant AB dye (ProZyme), and cleaned up on cleanup cartridges.”

Product: PNGase F (N-Glycanase)

Monitoring sialylation levels of Fc-fusion protein using size-exclusion chromatography as a process analytical technology tool

Liu, J; Chen, X; Fan, L; Deng, X; Fai Poon, H; Tan, WS; Liu, X; Biotechnol. Lett.; 2015; 37(7): 1371-7.

Snippet: “…In short, N-linked glycans were released from purified samples by overnight incubation with peptide-N-glycosidase F (ProZyme). The released glycans were recovered and labeled with the fluorophore 2-aminobenzamide (2-AB; ProZyme)…”

Product: peptide-N-glycosidase F, fluorophore 2-aminobenzamide (2-AB)

Expression and functional characterization of human lysosomal acid lipase gene (LIPA) mutation responsible for cholesteryl ester storage disease (CESD) phenotype

Rajamohan, F; Reyes, AR; Ruangsiriluk, W; Hoth, LR; Han, S; Caspers, N; Tu, M; Ward, J; Kurumbail, RG; Protein Expr. Purif.; 2015; 110: 22-9.

Snippet: “… aliquots and digested with 0.5 μg trypsin (Promega), endoproteinase Asp-N (Life Science) or chymotrypsin (Life Science) overnight at 37 °C. Approximately 0.4 μg of each digest was analyzed before and after treatment with 0.5 mU PNGase F (N-glycanase from ProZyme)…”

Product: PNGase F

Fully Automated Sample Preparation for Ultrafast N-Glycosylation Analysis of Antibody Therapeutics

Szigeti, M; Lew, C; Roby, K; Guttman, A; J Lab Autom; 2016; 21(2): 281-6.

Snippet: “… APTS and carbohydrate separation buffer (NCHO) were from SCIEX (Brea, California). The Agencourt CleanSeq magnetic beads were from Beckman Coulter (Brea, California), and the PNGase F enzyme and digestion reaction kit was from ProZyme, Hayward, California…”

Product: PNGase F

Galactose supplementation enhance sialylation of recombinant Fc-fusion protein in CHO cell: an insight into the role of galactosylation in sialylation

Liu, J; Wang, J; Fan, L; Chen, X; Hu, D; Deng, X; Poon, HF; Wang, H; Liu, X; Tan, WS; World J. Microbiol. Biotechnol.; 2015; 31(7): 1147-56.

Snippet: “…In short, N-linked glycans were released from purified samples by overnight incubation with Peptide-N-Glycosidase F (ProZyme, California). A cleaning Cartridge (ProZyme) was used to remove excess 2-AB…”

Product: Peptide-N-Glycosidase F, cleaning Cartridge

Structure of full-length human anti-PD1 therapeutic IgG4 antibody pembrolizumab

Scapin, G; Yang, X; Prosise, WW; McCoy, M; Reichert, P; Johnston, JM; Kashi, RS; Strickland, C; Nat. Struct. Mol. Biol.; 2015; 22(12): 953-8.

Snippet: “…100 µg of each antibody was treated with N-Glycanase-plus (ProZyme, GKE-5010B) in a total of volume of 50 µl of the digestion buffer provided with N-Glycanase-plus for 1 or 2 h at 37 °C...”

Product: N-Glycanase-plus

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies

Chan, KF; Shahreel, W; Wan, C; Teo, G; Hayati, N; Tay, SJ; Tong, WH; Yang, Y; Rudd, PM; Zhang, P; Song, Z; Biotechnol J; 2016; 11(3): 399-414.

Snippet: “…Trypsin was purchased from Promega Biosciences (San Luis Obispo, California). PNGase F was purchased from ProZyme, California. Hypercarb SPE cartridges and N-linked oligosaccharide standards were from Dextra Laboratories (Reading, UK)…”

Product: PNGase F

Human Carboxylesterase 2: studies on the role of glycosylation for enzymatic activity

Alves, M; Lamego, J; Bandeiras, T; Castro, R; Tomás, H; Coroadinha, AS; Costa, J; Simplicio, AL; Biochemistry and Biophysics Reports; 2015; 5: 105-110.

Snippet: “…Glycoproteins from concentrated supernatants were deglyco- sylated using peptide N-glycosidase F (PNGase F; ProZyme). The reactions were performed under two different …”

Product: peptide N-glycosidase F

Presence and Localization of Pro-and Mature Forms of Biglycan and Decorin in Human Costal Cartilage Derived from Chest Wall Deformities

Asmar, A; Werner, A; Kelly Jr, RE; Fecteau, A; Stacey, MW; Austin Journal of Musculoskeletal Disorders; 2015; 2(1): 2012-2017.

Snippet: “… To investigate cleavage of N-linked deglycosylationfrom core proteins, ABCase treated extracts were denatured at 100°C for 5 minutes then digested with PNGase-F using a N-Glycanase kit (ProZyme, Hayward, California) for 16 hours at 37°C following manufacturer's guidelines …”

Product: N-Glycanase kit

Comparison of Follicle-Stimulating Hormone Glycosylation Microheterogenity by Quantitative Negative Mode Nano-Electrospray Mass Spectrometry of Peptide-N Glycanase-Released Oligosaccharides

Bousfield, GR; Butnev, VY; White, WK; Hall, AS; Harvey, DJ; J Glycomics Lipidomics; 2015; 5(1)

Snippet: “…Each FSH sample was then incubated overnight at 37°C with 2.5 mU peptide-N-glycanase F (PNGaseF) obtained from ProZyme, Hayward, California. PNGaseF-released oligosaccharides were …”

Product: peptide-N-glycanase F

Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation

Charter, NW; Horecka, J; Loh, CY; Doan, A; Wehrman, T; Olson, KR; Methods Mol. Biol.; 2015; 1314: 51-61.

Snippet: “… PNGase Treatment. 1. The PNGase F kit (ProZyme) was used for enzymatic removal of N-linked glycans from glycoproteins. In this example, cells were lysed with the ProZyme Kit denaturing buffer at 10 million cells/mL. …”

Product: Glyko PNGase F kit

Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures

Gomes, J; Gomes-Alves, P; Carvalho, SB; Peixoto, C; Alves, PM; Altevogt, P; Costa, J; Biomolecules; 2015; 5(3): 1741-61.

Snippet: “…After cooling, the beads were incubated at 37 °C overnight, either with 5 mU Endo H (Roche Diagnostics GmbH) in 50 mM sodium citrate pH 5.5, or with 2.5 mU PNGase F (ProZyme, Hayward, California) in 1% Nonidet P-40 and 50 mM sodium phosphate pH 7.5, 10 mM EDTA...”

Product: PNGase F

Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis

Mareninova, OA; Sendler, M; Malla, SR; Yakubov, I; French, SW; Tokhtaeva, E; Vagin, O; Oorschot, V; Lüllmann-Rauch, R; Blanz, J; Dawson, D; Klumperman, J; Lerch, MM; Mayerle, J; Gukovsky, I; Gukovskaya, AS; Cell Mol Gastroenterol Hepatol; 2015; 1(6): 678-694.

Snippet: “… 5 minutes in the presence of 0.5% sodium dodecyl sulfate, and then treated with peptide:N-glycosidase (PNGase) F from Flavobacterium meningosepticum (New England BioLabs, Ipswich, MA) or with endonuclease H from Streptomyces plicatus (ProZyme) …”

Product: peptide:N-glycosidase (PNGase) F

Physical, Inorganic, and Analytical

Prien, JM; Stöckmann, H; Albrecht, S; Martin, SM; Varatta, M; Furtado, M; Hosselet, S; Wang, M; Formolo, T; Rudd, P; Schiel, JE; Chapter (Book); 2015;: .

Snippet: “…Peptidase N-glycosidase F (PNGase F), sialidase A, β-(1-4)-galactosidase, and β-N-hexosaminidase was purchased from ProZyme, Hayward, California. GlykoPrep H and CU solid phase extraction columns were purchased from ProZyme, Hayward, California...”

Product: Peptidase N-glycosidase F (PNGase F), sialidase A, β-(1-4)-galactosidase, and β-N-hexosaminidase

The N-Glycosylation of immunoglobulin G as a novel biomarker of Parkinson's disease

Russell, AC; Thesis; 2015;: .

Snippet: “…After drying, proteins were denatured with the addition of 30 μL 1.33% sodium dodecyl sulfate (w/v) (Invitrogen, Californiarlsbad, California) and incubated at 60 °C for 10 min. Subsequently, 10 μL 4% Igepal-CA630 (Sigma-Aldrich, St. Louis, MO) and 0.5 mU of PNGase F (ProZyme, Hayward, California) in 10 μL 5× PBS were added to the samples...”

Product: PNGase F

Ultrahigh Throughput, Ultrafiltration-Based N-Glycomics Platform for Ultraperformance Liquid Chromatography (ULTRA3)

Stöckmann, H; Duke, RM; Millán Martín, S; Rudd, PM; Anal. Chem.; 2015; 87(16): 8316–8322.

Snippet: “…Exoglycosidase and PNGase F enzymes were obtained from ProZyme: PNGase F (GKE-5006D), Arthrobacter Sialidase (GK80090), Bovine Kidney Fucosidase (GK5006), Bovine Testes Galactosidase (GKX-5013), N-acetyl hexosaminidase (GK80050), and Jack Bean Mannosidase (GKX-5003)...”

Product: PNGase F, Arthrobacter Sialidase (ABS), Bovine Kidney Fucosidase (BKF), Bovine Testes Galactosidase (BTG), N-acetyl hexosaminidase (GUH), and Jack Bean Mannosidase (JBM)

Sample preparation for N-glycosylation analysis of therapeutic monoclonal antibodies by electrophoresis

Szekrényes, Á; Partyka, J; Varadi, C; Krenkova, J; Foret, F; Guttman, A; Methods Mol. Biol.; 2015; 1274: 183-95.

Snippet: “… Peptide-N-glycosidase F (ProZyme, Hayward, California). Acknowledgments. The authors acknowledge the kind support of Beckman Coulter, Inc., PhyNexus, Inc., and ProZyme, Inc. …”

Product: Peptide-N-glycosidase F

Analysis of protein ligand-receptor binding by photoaffinity cross-linking

Wu, L; Xu, B; Curr Protoc Protein Sci; 2015; 79: 19.26.1-14.

Snippet: “…  Anti-His antibody (Penta-His antibody; Qiagen), Restore Western Blot Stripping buffer (Thermo Fisher Scientific), Peptide-N-glycosidase F (N-Glycanase; PNGase F; ProZyme)…”

Product: Peptide-N-glycosidase F

Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity

Alves, M; Lamego, J; Bandeiras, T; Castro, R; Tomas, H; Coroadinha, AS; Costa, J; Simplicio, AL Biochemistry and Biophysics Reports; 2016; 5: 105-110.

Snippet: “… and quantified for total protein concentration. Glycoproteins from concentrated supernatants were deglycosylated using peptide N-glycosidase F (PNGase F; ProZyme). The reactions were performed under two different …”

Product: peptide N-glycosidase F (PNGase F)

IgG and IgM glycosylation patterns in patients undergoing image-guided tumor ablation

Breen, LD; Pučić-Baković, M; Vučković, F; Reiding, K; Trbojević-Akmačić, I; Gajdošik, MŠ; Cook, MI; Lopez, MJ; Wuhrer, M; Camara, LM; Andjelković, U; Dupuy, DE; Josić, D; Biochim. Biophys. Acta; 2016; 1860(8): 1786-94.

Snippet: “… Subsequently, 4% (v/v) Igepal CA-630 non-ionic detergent was added to the mixture, and N-glycans were released with the addition of N-glycosidase F (ProZyme, California) in PBS and overnight incubation at 37 °C. Released N-glycans were fluorescently labeled …”

Product: N-glycosidase F

Glycomic analysis of gastric carcinoma cells discloses glycans as modulators of RON receptor tyrosine kinase activation in cancer

Mereiter, S; Magalhães, A; Adamczyk, B; Jin, C; Almeida, A; Drici, L; Ibáñez-Vea, M; Gomes, C; Ferreira, JA; Afonso, LP; Santos, LL; Larsen, MR; Kolarich, D; Karlsson, NG; Reis, CA; Biochim. Biophys. Acta; 2016; 1860(8): 1795-808.

Snippet: “… N-linked oligosaccharides were released in the spinfilter using 20 μl 50 mM NH4HCO3 and PNGase F (5 mU, ProZyme, Hayward, California) with incubation at 37 °C overnight. Subsequently, the N-glycans were collected by washing 3 times with 20 μl H2O and dried in Speedvac. …”

Product: PNGase F

Glycomic and sialoproteomic data of gastric carcinoma cells overexpressing ST3GAL4

Mereiter, S; Magalhães, A; Adamczyk, B; Jin, C; Almeida, A; Drici, L; Ibáñez-Vea, M; Larsen, MR; Kolarich, D; Karlsson, NG; Reis, CA; Data Brief; 2016; 7: 814-33.

Snippet: “… N-linked oligosaccharides were released in the spinfilter using 20 µl 50 mM NH4HCO3 and PNGase F (5 mU, ProZyme, Hayward, California) with incubation at 37 °C overnight. Subsequently, the N-glycans were collected by washing 3 times with 20 µl H2O and dried in Speedvac. …”

Product: PNGase F

Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation

Pavić, T; Gudelj, I; Keser, T; Pučić-Baković, M; Gornik, O; Biochim. Biophys. Acta; 2016; 1860(8): 1710-5.

Snippet: “… Subsequently, 10 μl of 4% Igepal-CA630 (Sigma–Aldrich, St. Louis, MO) and 1.25 mU of PNGase F (ProZyme, Hayward, California) in 10 μl of 5 × PBS were added to the samples. All enzymes were purchased from ProZyme, Hayward, California. …”

Product: PNGase F