Citations - GlykoPrep Kits and Reagents

A Markov chain model for N-linked protein glycosylation--towards a low-parameter tool for model-driven glycoengineering

Spahn, PN; Hansen, AH; Hansen, HG; Arnsdorf, J; Kildegaard, HF; Lewis, NE; Metab. Eng.; 2016; 33: 52-66.

Snippet: “…. N-glycans from retained proteins were released and fluorescently labeled with GlykoPrep Rapid N-Glycan kit (ProZyme, Hayward, California)...”

Product: GlykoPrep N-Glycan kit

Comparative Assessment of Glycosylation of a Recombinant Human FSH and a Highly Purified FSH Extracted from Human Urine

Wang, H; Chen, X; Zhang, X; Zhang, W; Li, Y; Yin, H; Shao, H; Chen, G; J. Proteome Res.; 2016; 15(3): 923-32.

Snippet: “…Glycoclean columns and 2-AB labeling kit were obtained from ProZyme, California...”

Product: 2-AB labeling kit

Consistency of quality attributes for the glycosylated monoclonal antibody Humira® (adalimumab)

Tebbey, PW; Varga, A; Naill, M; Clewell, J; Venema, J; MAbs; 2015; 7(5): 805-11.

Snippet: “… and quantification of the oligosaccharides on the conserved N-linked glycosylation site of Humira® was performed by N-glycanase cleavage and normal phase separation (NP-HPLC) with fluorescent detection (Signal™ 2-Aminobenzamide labeling kit, ProZyme …”

Product: 2-AB labeling kit

A multi-pronged investigation into the effect of glucose starvation and culture duration on fed-batch CHO cell culture

Fan, Y; Jimenez Del Val, I; Müller, C; Lund, AM; Sen, JW; Rasmussen, SK; Kontoravdi, C; Baycin-Hizal, D; Betenbaugh, MJ; Weilguny, D; Andersen, MR; Biotechnol. Bioeng.; 2015; 112(10): 2172-84.

Snippet: “…mAb Glycoprofiling was performed with an in-house HPLC analysis method using InstantAB labeled (ProZyme, Hayward, California) glycans…”

Product: GlykoPrep N-Glycan kit with InstantAB

Amino acid and glucose metabolism in fed-batch CHO cell culture affects antibody production and glycosylation

Fan, Y; Jimenez Del Val, I; Müller, C; Wagtberg Sen, J; Rasmussen, SK; Kontoravdi, C; Weilguny, D; Andersen, MR; Biotechnol. Bioeng.; 2015; 112(3): 521-35.

Snippet: “… Glycoprofiling of IgG. IgG glycoprofiling was carried out using GlykoPrep® InstantAB™ kit (ProZyme, Hayward, California) for quantifying the total of each N-glycans. Digestion, labeling and cleanup of N-glycans from IgG was performed according to the manufacturer's instructions. …”

Product: GlykoPrep N-Glycan kit with InstantAB

Glycoprofiling effects of media additives on IgG produced by CHO cells in fed-batch bioreactors

Kildegaard, HF; Fan, Y; Sen, JW; Larsen, B; Andersen, MR; Biotechnol. Bioeng.; 2016; 113(2): 359-66.

Snippet: “… The assigned peaks were quantitated in the Dionex-Chromeleon Chromatography Data System. Instant-AB Labeled Glycoprofiling. Fifty micrograms of purified IgG per sample have been processed according to instruction of iGlykoPrep InstantAB kit (ProZyme). …”

Product: GlykoPrep N-Glycan kit with InstantAB

Identifying the differences in mechanisms of mycophenolic acid controlling fucose content of glycoproteins expressed in different CHO cell lines

Zhang, A; Tsang, VL; Markely, LR; Kurt, L; Huang, YM; Prajapati, S; Kshirsagar, R; Biotechnol. Bioeng.; 2016;: .

Snippet: “… N-Glycan Analysis. Proteins were purified using an in-house affinity chromatography resin. N-glycans were released, fluorescently labeled, and purified using the GlykoPrep 2AB kit (ProZyme GP96NG-AB) as described in the user manual. …”

Product: GlykoPrep N-Glycan kit with 2AB

Chemoenzymatic method for glycomics: Isolation, identification, and quantitation

Yang, S; Rubin, A; Eshghi, ST; Zhang, H; Proteomics; 2016; 16(2): 241-56.

Snippet: “…A rapid glycoprotein sample preparation platform, GlykoPrep, has been reported by ProZyme, for streamlined N-glycan preparation including release, purification and labeling...”

Product: GlykoPrep N-Glycan kit

High-throughput analysis of immunoglobulin G glycosylation

Trbojević-Akmačić, I; Vilaj, M; Lauc, G; Expert Rev Proteomics; 2016; 13(5): 523-34.

Snippet: “…the sensitivity of detection: the intensities of 2-AA-labeled glycans were higher than that of 2-AB-labeled counterparts in chromatographic analysis as well as in RP-LC-MS analysis. Both 2-AB and 2-AA are commercially available as kits from number of manufacturers, for example, GlycoProfile 2-AA/2-AB labeling kits (from Sigma-Aldrich), GlykoPrep kit (from ProZyme)...”

Product: GlykoPrep N-Glycan kit

State-of-the-art technologies for rapid and high-throughput sample preparation and analysis of N-glycans from antibodies

Aich, U; Lakbub, J; Liu, A; Electrophoresis; 2016; 37(11): 1468-88.

Snippet: “… developed for 2AB and instant AB labeled N-glycans from glycoproteins based on automated AssayMAP robotic platform. This workflow utilizes ProZyme's GlykoPrep-plus chemistry kits with customized easy-to-run software and reagent preparation SOP's. The automation …”

Product: GlykoPrep N-Glycan kit

Bioreactor process parameter screening utilizing a Plackett-Burman design for a model monoclonal antibody

Agarabi, CD; Schiel, JE; Lute, SC; Chavez, BK; Boyne, MT; Brorson, KA; Khan, MA; Read, EK; J Pharm Sci; 2015; 104(6): 1919-28.

Snippet: “…Released glycans were purified using GlykoPrep H cartridges (ProZyme, Hayward, California)…”

Product: GlykoPrep H cartridges

Characterization of N-glycosylation profiles from mammalian and insect cell derived chikungunya VLP

Lancaster, C; Pristatsky, P; Hoang, VM; Casimiro, DR; Schwartz, RM; Rustandi, R; Ha, S; J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.; 2016;: .

Snippet: “… Louis, MO). 2-Aminobenzamide (2AB) labeling kit, α(1-2,3,6) mannosidase, α(1-2,3,4,6) fucosidase, β(1-3,4) galactosidase, and α(2-3,6,8,9) sialidase A (each including its own 5× Reaction Buffer) were purchased from ProZyme, Hayward, California. …”

Product: 2-Aminobenzamide (2AB) labeling kit, α(1-2,3,6) mannosidase, α(1-2,3,4,6) fucosidase, β(1-3,4) galactosidase, α(2-3,6,8,9) sialidase A

The analyses of fluorescently labeled biomolecules using ultraviolet-wavelength fluorophore-assisted laser desorption/ionization-mass spectrometry (UV FALDI-MS)

West, RE; Jacobs, JB; Isailovic, D; International Journal of Mass Spectrometry; 2015; 389: 39-46.

Snippet: “… These peptides include 5-carboxytetramethylrhodamine (TAMRA)-angiotensin II, Californiarboxyfluorescein (FAM)-bombesin, and FAM-angiotensin II. The 2-aminobenzamide (2-AB) labeling kit for glycans was purchased from ProZyme, Hayward, California. …”

Product: 2-aminobenzamide (2-AB) labeling kit

Diversity in host clone performance within a Chinese hamster ovary cell line

O'Callaghan, PM; Berthelot, ME; Young, RJ; Graham, JW; Racher, AJ; Aldana, D; Biotechnol. Prog.; 2015; 31(5): 1187-200.

Snippet: “… The oligosaccharides were then separated from the protein, concentrated using a speed vac, labeled with the fluorophore 2-aminobenzamide (2-AB) using the GlykoPrep Rapid 2-AB kit (ProZyme) and digested with α-sialidase. …”

Product: GlykoPrep N-Glycan kit with 2-AB

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

Grav, LM; Lee, JS; Gerling, S; Kallehauge, TB; Hansen, AH; Kol, S; Lee, GM; Pedersen, LE; Kildegaard, HF; Biotechnol J; 2015; 10(9): 1446-56.

Snippet: “…N-glycans from retained proteins were released and fluorescently labeled with GlykoPrep Rapid N-Glycan kit (ProZyme, Hayward, California) according to the manufacturer's instructions using 2-aminobenzamide (2-AB) as a fluorescent label. …”

Product: GlykoPrep N-Glycan kit with 2-AB

Pharmacokinetic Comparability of a Biosimilar Trastuzumab Anticipated from Its Physicochemical and Biological Characterization

Miranda-Hernández, MP; López-Morales, CA; Piña-Lara, N; Perdomo-Abúndez, FC; Pérez, NO; Revilla-Beltri, J; Molina-Pérez, A; Estrada-Marín, L; Flores-Ortiz, LF; Ruiz-Argüelles, A; Medina-Rivero, E; Biomed Res Int; 2015; 2015: 874916.

Snippet: “…Ammonium formate (CH5NO2 ) and formic acid were obtained from Sigma-Aldrich (Saint Louis, MO). 2-Aminobenzamide (2-AB) was obtained from ProZyme, Hayward, California …”

Product: 2-Aminobenzamide (2-AB)

Optimizing Chinese hamster ovary cell line development via targeted control of N-glycosylation

Alves, CS; Prajapati, S; Pharmaceutical Bioprocessing; 2015;: .

Snippet: “… The short HILIC method combined with high-throughput N-glycan sample preparation kits, such as GlykoPrep (ProZyme)fully automated on Bravo™ (Agilent) or RapiFluor-MS (Waters) enables N-glycan structure analysis of many samples as would be seen during …”

Product: GlykoPrep N-Glycan kit

Assessment of physicochemical properties of rituximab related to its immunomodulatory activity

Miranda-Hernández, MP; López-Morales, CA; Ramírez-Ibáñez, ND; Piña-Lara, N; Pérez, NO; Molina-Pérez, A; Revilla-Beltri, J; Flores-Ortiz, LF; Medina-Rivero, E; J Immunol Res; 2015; 2015: 910763.

Snippet: “… Louis, MO). 2-Aminobenzamide (2-AB) was obtained from ProZyme Inc., Hayward, California; PNGase F was purchased from New England Biolabs (Woburn, MA) and Human IgG-Fc antibody from Bethyl Laboratories Inc. (Montgomery, TX). …”

Product: 2-Aminobenzamide

N-Glycan Composition Profiling for Quality Testing of Biotherapeutics

Taron, CH; Duke, R. BioPharm International; 2015; 28(12): .

Snippet: “… These compounds are based on the reaction of activated fluorescent carbamates (eg, InstantAB from ProZyme, 6-aminoquinoyl N-hydroxysuccinimidyl carbamate [AQC], and RapiFluor from Waters) with glycosylamine…”

Product: GlykoPrep N-Glycan kit with InstantAB