Citations - Glycan Standards

Comparison of the bifidogenic activity of human and bovine milk N-glycome

Wang WL, Du YM, Wang W, Conway LP, Cai ZP, Voglmeir J, Liu L. Journal of Functional Foods; 2017 June;33:40–51.

Snippet: “…GLY3 standards (Man5GlcNAc2, Man3GlcNAc5, Man3Gal4GlcNAc6) were purchased from ProZyme, California. Unless otherwise stated, all H2O used was ultrapure (ie ≥18.2 MΩ and ≤5 ppb TOC). Page 9. 9 Tissue sectioning …”

Product: glycan standards

N-Glycan MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissue Enables the Delineation of Ovarian Cancer Tissues

Everest-Dass, AV; Briggs, MT; Kaur, G; Oehler, MK; Hoffmann, P; Packer, NH; Mol. Cell Proteomics; 2016; 15(9):3003-16.

Snippet: “…GLY3 standards (Man5GlcNAc2, Man3GlcNAc5, Man3Gal4GlcNAc6) were purchased from ProZyme, California. Unless otherwise stated, all H2O used was ultrapure (ie ≥18.2 MΩ and ≤5 ppb TOC). Page 9. 9 Tissue sectioning …”

Product: GLY3 standards (Man5GlcNAc2, Man3GlcNAc5, Man3Gal4GlcNAc6)

Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles—part 1: Separation-based methods

Reusch, D; Haberger, M; Maier, B; Maier, M; Kloseck, R; Zimmermann, B; Hook M; Szabo, Z; Tep, S; Wegstein, J; Alt, N; Bulau, P; Wuhrer, M; MAbs; 2015; 7(1): 167-79.

Snippet: “… The study involved 3 laboratories: a biopharmaceutical company (Roche Diagnostics), an academic research laboratory (Leiden University Medical Center) and a vendor of tools for glycan analysis (ProZyme, Inc.). The same mAb sample was analyzed 6-fold on 2 different days. …”

Product: Glycan analysis

N-glycosylation profile analysis of Trastuzumab biosimilar candidates by Normal Phase Liquid Chromatography and MALDI-TOF MS approaches

Sanchez-De Melo, I; Grassi, P; Ochoa, F; Bolivar, J; García-Cózar, FJ; Durán-Ruiz, MC; J Proteomics; 2015; 127(Pt B): 225-33.

Snippet: “… A–75% B for a further 12 min. N-linked glycan structures were assigned to peaks based on comparison to reference structures (2AB-labeled human IgG N-glycans, ProZyme, California). G0, G1, and G2 refer to complex …”

Product: 2AB-labeled human IgG N-glycans

A congenital disorder of deglycosylation: Biochemical characterization of N-glycanase 1 deficiency in patient fibroblasts

He, P; Grotzke, JE; Ng, BG; Gunel, M; Jafar-Nejad, H; Cresswell, P; Enns, GM; Freeze, HH; Glycobiology; 2015; 25(8): 836-44.

Snippet: “… PNGase F was prepared in our laboratory. Glyko 2-AB (glucose homopolymer) standard was purchased from ProZyme, Hayward, California. Cell culture. Control and patient fibroblasts were cultured in DMEM (with 1 g/L glucose) with 10% FBS. …”

Product: Glyko 2-AB (glucose homopolymer) standard

The Fucosidase-Pool of Emticicia oligotrophica: Biochemical Characterisation and Transfucosylation Potential

Liu, S; Kulinich, A; Cai, ZP; Ma, HY; Du, YM; Lv, YM; Liu L, Voglmeir J. Glycobiology; 2016; 26(8):871-9

Snippet: “…2-fucosyllactose (2-FL), 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP I), lacto-N-fucopentaose III (LNFP III), lacto-N-fucopentaose V (LNFP V), lacto-N-difucohexaose I (LNDFH I), lacto-N-difucohexaose II (LNDFH II), lacto-N-neotetraose (LNnT) and the mono-sialylated, biantennary N-glycan standards with (A1F) and without α1,6-linked core fucose (A1) were obtained from ProZyme …”

Product: Glycan standards

Profiling N-linked oligosaccharides from IgG by high-performance anion-exchange chromatography with pulsed amperometric detection

Rohrer, JS; Basumallick, L; Hurum, DC; Glycobiology; 2016; 26(6): 582-91.

Snippet: “… WI. The oligomannose species M6 and M5, as well as the complex N-linked glycans G0, G1F, G2bF, A1F and A2F standards, were purchased from ProZyme, Hayward, California. N-linked oligosaccharide release from IgG. N-linked …”

Product: Oligomannose species M6 and M5, complex N-linked glycans G0, G1F, G2bF, A1F and A2F standards

MALDI imaging mass spectrometry of N-linked glycans on formalin-fixed paraffin-embedded murine kidney

Gustafsson, OJ; Briggs, MT; Condina, MR; Winderbaum, LJ; Pelzing, M; McColl, SR; Everest-Dass, AV; Packer, NH; Hoffmann, P; Anal Bioanal Chem; 2015; 407(8): 2127-39.

Snippet: “… Indium tin oxide (ITO) slides were purchased from Bruker Daltonics, while PEN membrane slides were from MicroDissect (Herborn, Germany). GLY3 standards (see Table 1) were purchased from ProZyme (CA). 1257.4225. 1 pmol/μL. ProZyme, California. Man 3 GlcNAc 5. …”

Product: GLY3 standards

Comparison of Traditional 2-AB Fluorescence LC-MS/MS and Automated LC-MS for the Comparative Glycan Analysis of Monoclonal Antibodies

Schiel, JE; Rogstad, SM; Boyne, MT; J Pharm Sci; 2015; 104(8): 2464-72.

Snippet: “… A rejected ion mass list of common contaminants was used that included m/z = 391.2, 413.2, and 427.2 Da. The ion optics of the mass spectrometer was tuned to a 1 pmol/μL (water) solution of 2-AB G0 glycan obtained from ProZyme, Hayward, California. …”

Product: 2-AB G0 glycan

An Integrated Solution-Based Rapid Sample Preparation Procedure for the Analysis of N-Glycans From Therapeutic Monoclonal Antibodies

Aich, U; Liu, A; Lakbub, J; Mozdzanowski, J; Byrne, M; Shah, N; Galosy, S; Patel, P; Bam, N; J Pharm Sci; 2016; 105(3): 1221-32.

Snippet: “… Deionized (DI) water, 18 MΩ-cm resistivity or better was used throughout for the sample preparation and analysis. 2AA-labeled glycan standards used for calibration were obtained from ProZyme (Haywood, California)…”

Product: 2AA-labeled glycan standards

Tuning a MAb glycan profile in cell culture: Supplementing N-acetylglucosamine to favour G0 glycans without compromising productivity and cell growth

Blondeel, EJ; Braasch, K; McGill, T; Chang, D; Engel, C; Spearman, M; Butler, M; Aucoin, MG; J. Biotechnol.; 2015; 214: 105-12.

Snippet: “… 50 over 48 min. Peaks were calibrated with a 2-AB labeled glucose ladder and glycan standards (ProZyme, Hayward, California) and compared to a glycan database (Glycobase ( Campbell et al., 2008)). Glycan structures were confirmed …”

Product: Glycan standards

Identification of potential pancreatic cancer serum markers: Increased sialyl-Lewis X on ceruloplasmin

Balmaña, M; Sarrats, A; Llop, E; Barrabés, S; Saldova, R; Ferri, MJ; Figueras, J; Fort, E; de Llorens, R; Rudd, PM; Peracaula, R; Clin. Chim. Acta; 2015; 442: 56-62.

Snippet: “… The 2AB-labelled glycans were digested in 10 μl of 50 mM sodium acetate buffer, pH 5.5, for 18 h at 37 °C, using arrays of the following enzymes (all purchased from ProZyme, California) at the indicated concentrations: ABS—Arthrobacter ureafaciens sialidase (EC 3.2 …”

Product: 2AB-labelled glycans

Microscale purification of antigen-specific antibodies

Brown, EP; Normandin, E; Osei-Owusu, NY; Mahan, AE; Chan, YN; Lai, JI; Vaccari, M; Rao, M; Franchini, G; Alter, G; Ackerman, ME; J. Immunol. Methods; 2015; 425: 27-36.

Snippet: “… The eluted fraction was collected for HPLC analysis. Labeled N-glycan samples injected were diluted 1:3 with acetonitrile with a volume at 100 μL. GlyKO 2-AB-human IgG N linked library (ProZyme) was used as a standard. …”

Product: 2-AB-human IgG N linked library

A method for high-throughput, sensitive analysis of IgG Fc and Fab glycosylation by capillary electrophoresis

Mahan, AE; Tedesco, J; Dionne, K; Baruah, K; Cheng, HD; De Jager, PL; Barouch, DH; Suscovich, T; Ackerman, M; Crispin, M; Alter, G; J. Immunol. Methods; 2015; 417: 34-44.

Snippet: “… To identify peaks, including high mannose structures, known glycan standards (ProZyme) were labeled with APTS as described for IgG glycans and spiked into human IgG-derived samples. ProZyme standards and IgG-derived …”

Product: Glycan standards

Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures

Seo, JS; Min, BS; Kwon, YB; Lee, SY; Cho, JM; Park, KH; Yang, YJ; Maeng, KE; Chang, SJ; Kim, DI; J. Biosci. Bioeng.; 2016; 121(3): 317-24.

Snippet: “… Each glycan species peak in HPAEC-PAD was identified by using an internal glycan standard species, such as G0 (GKC-004300, ProZyme), G0F (GKC-004301, ProZyme), G1F (GKC-014301, ProZyme), G2F (GKC-024301, ProZyme), and Man5 (GKM-002500, ProZyme). …”

Product: Glycan Standards

A global RNA-seq-driven analysis of CHO host and production cell lines reveals distinct differential expression patterns of genes contributing to recombinant antibody glycosylation

Könitzer, JD; Müller, MM; Leparc, G; Pauers, M; Bechmann, J; Schulz, P; Schaub, J; Enenkel, B; Hildebrandt, T; Hampel, M; Tolstrup, AB; Biotechnol J; 2015; 10(9): 1412-23.

Snippet: “… A BEH glycan column (Waters, Manchester, UK) was used in conjunction with a proprietary acetonitrile elution gradient method. Glycan peaks were assigned to sugar subspecies according to standards provided by ProZyme, Hayward, California. …”

Product: Glycan Standards

Effect of Culture Conditions on the Glycosylation Pattern of mAb

Aghamohseni, H; Thesis; 2015.

Snippet: “…Peaks were calibrated with a 2-AB labeled glucose ladder and glycan standards (ProZyme) (Guile et al., 1996) and compared to a glycan database ( for preliminary identification (Figure 1). Further structural assignments were performed by exoglycosidase array analysis 47 (Royle et al. 2006) using sialidase A (recombinant from A. ureafaciens), bovine kidney α- fucosidase, bovine testis β-galactosidase, Jack Bean β-N-acetylhexoaminidase (ProZyme)...”

Product: glycan standards, sialidase A (recombinant from A. ureafaciens), bovine kidney α- fucosidase, bovine testis β-galactosidase, Jack Bean β-N-acetylhexoaminidase

Alpha-1-acid glycoprotein as a potential biomarker of breast cancer in at risk individuals

Dewar, EL; Thesis; 2015.

Snippet: “…A reference profile comprised of approximately 5μg of a human N-linked oligosaccharide library purchased from ProZyme, Californiawas analysed for comparison with commercial AGP and patient-derived sample profiles...”

Product: Human N-linked oligosaccharide library

Overcome Challenges to Successful Manufacture of Biosimilars through Media and Feed Screening and Cell Culture Process Optimization

Zhang, M; Hill, T. Journal of Bioanalysis & Biomedicine; 2016; 8(2): 023-027.

Snippet: “… up to remove excess dye. Identification of the glycans was performed by comparison against a 2-AB labeled Human IgG N-linked library from ProZyme, spiked with 2-AB labeled Man5 standard. The separation of glycans was …”

Product: 2-AB labeled Human IgG N-linked library