Citations - Deglycosylation Kits

Manipulating TLR Signaling Increases the Anti-tumor T Cell Response Induced by Viral Cancer Therapies

Rojas, JJ; Sampath, P; Bonilla, B; Ashley, A; Hou, W; Byrd, D; Thorne, SH; Cell Rep; 2016; 15(2): 264-73.

Snippet: “…For deglycosylation of viruses, an enzymatic deglycosylation kit was used (ProZyme)...”

Product: Enzymatic deglycosylation kit

Detection of p75NTR Trimers: Implications for Receptor Stoichiometry and Activation

Anastasia, A; Barker, PA; Chao, MV; Hempstead, BL; J. Neurosci.; 2015; 35(34): 11911-20.

Snippet: “… Enzymatic deglycosylation. The deglycosylation kit was purchased from ProZyme; 5 μl of 5× Tris reaction buffer provided by the manufacturer was added to 20 μl of lysates containing 50 μg of protein (without any other detergent or denaturing agent). …”

Product: Enzymatic deglycosylation kit

Identification of the critical attribute(s) of EBV gp350 antigen required for elicitation of a neutralizing antibody response in vivo

Servat, E; Ro, BW; Cayatte, C; Gemmell, L; Barton, C; Rao, E; Lin, R; Zuo, F; Woo, JC; Hayes, GM; Vaccine; 2015; 33(48): 6771-7.

Snippet: “… dialyzed in PBS. 2.3. Enzymatic deglycosylation of gp350. Recombinant gp350 was enzymatically deglycosylated using the GlycoPro Enzymatic Deglycosylation Kit per manufacturer's protocol (ProZyme). For deglycosylation …”

Product: Enzymatic deglycosylation kit

Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, molecular cloning and gene expression in Aspergillus sojae

Satake, R; Ichiyanagi, A; Ichikawa, K; Hirokawa, K; Araki, Y; Yoshimura, T; Gomi, K; J. Biosci. Bioeng.; 2015; 120(5): 498-503.

Snippet: “… Science Institute Inc. MpGDH was deglycosylated using the Enzymatic Deglycosylation Kit (ProZyme, Hayward, California) according to the manufacturer's instructions. Measurement of enzyme stability. Purified MpGDH was diluted …”

Product: Enzymatic deglycosylation kit

Cellular dissection of zebrafish hematopoiesis

Stachura, DL; Traver, D; Methods Cell Biol.; 2016; 133: Nov-53.

Snippet: “…To test this hypothesis, we removed both O-linked and N-linked sugars from WKM using a deglycosylation kit (ProZyme), and then incubated these cells with previously positive mAbs...”

Product: Enzymatic deglycosylation kit

Porphyromonas gingivalis Type IX Secretion Substrates Are Cleaved and Modified by a Sortase-Like Mechanism

Gorasia, DG; Veith, PD; Chen, D; Seers, CA; Mitchell, HA; Chen, YY; Glew, MD; Dashper, SG; Reynolds, EC; PLoS Pathog.; 2015; 11(9): e1005152.

Snippet: “…The samples were transferred to glass vials and freeze dried thoroughly. Deglycosylation was performed using the protocol from the manufacturer of ProZyme Glycofree Chemical deglycosylation kit (GKK-500)…”

Product: Chemical deglycosylation kit (GKK-500)

Monoclonal Antibody EIV-E12 Recognizes a Glycoprotein Antigen, Which Differs from the B-Cell-Specific chB6 (Bu-1) Antigen

Nuthalapati, N; Alugubelly, N; Felfoldi, B; Bodi, I; Fejszak, N; Pharr, GT; Olah, I; International Journal of Poultry Science; 2015; 14(8): 479-484.

Snippet: “…Protein deglycosylation and western blot analysis: Proteins were deglycosylated with the enzymatic deglycosylation kit according to the manufacturer's protocol (ProZyme, GK80110). Briefly, whole …”

Product: Enzymatic deglycosylation kit