1 mg of purified goat IgG was conjugated with varying amounts of SMCC-xlAPC (from 0.8 to 3.2 mg). The conjugates formed were clarified in a microfuge and the supernatants purified on a Biogel A0.5M column. For each conjugation the following were determined:
Note: a + b + c = 100%.
Actual results would vary to some extent from conjugation to conjugation depending on the precise concentrations of the reactants and the particular antibody or target protein. However, some general conclusions can be drawn:
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