Streptavidin-R-Phycoerythrin [PJRS34]

Price:
$ 275.00

Description

PhycoLink® Streptavidin-R-Phycoerythrin

PhycoLink® Streptavidin-R-Phycoerythrin. PhycoPro™ R-PE was conjugated to SA26, recombinant Streptavidin-plus®. PJRS34 is specifically manufactured as a low background conjugate for research applications utilizing bead-based assays such as the Luminex® platform.

Size: 1 mg, 10 mg

Product Code: PJRS34

FAQs

Q. ProZyme has a number of Streptavidin-R-Phycoerythrin conjugates. How are they different and which one should I use?

A. Our range of Streptavidin-R-Phycoerythrin (SA-RPE) conjugates differ in overall size. We recommend that you test our conjugates to find out which is the best for your system. 

Please contact us to set up a sample to test in your application or order the SA-RPE sampler kit, PJ3SX, that contains the following conjugates (0.25mg ea): PJRS20PJRS301PJRS34PJRS25PJRS27PJ39S

 Product Size Application

PJRS301†  PJRS20  PJRS34

Large Luminex and other bead based assays  

PJRS25†† PJRS27

Medium Tetramer
PJ39S Small Flow cytometry

 PJRS301 is our newest conjugate, developed for long-term signal consistency.

PJRS25 is used in the NIH (Emory) Tetramer Core Facility protocol published online, along with our streptavidin-APC (allophycocyanin) conjugate PJ27S. 

Q. What dilution should I use for your SA-RPE conjugates?

A. We don’t have set protocols for our SA-RPE conjugates, as this may depend on your system & application. Please contact us if you are interested in a sample to test.

As a frame of reference, for our SA-RPE Luminex QC assay we dilute to 10 μg/ml and add 25 μl (0.25 μg) per well. Dilution of SA-RPE to 2-20 μg/ml for addition to the wells is a good starting point for Luminex.

Product Citations
  1. Leonard JD, Gilmore DC, Dileepan T, Nawrocka WI, Chao JL, Schoenbach MH, Jenkins MK, Adams EJ, Savage PA.  Identification of Natural Regulatory T Cell Epitopes Reveals Convergence on a Dominant Autoantigen.   2017 Jul 18;47(1):107-117.e8. doi: 10.1016/j.immuni.2017.06.015. Epub 2017 Jul 11.
Safety

Product Safety Documentation for PJRS34:

Product/Part No. Description
PJRS34 Streptavidin-R-Phycoerythrin