Streptavidin-R-Phycoerythrin [PJRS27]

Price:
$ 275.00

Description

PhycoLink® Streptavidin-R-Phycoerythrin.

PhycoPro™ R-PE was conjugated to Streptavidin (Streptomyces avidinii). PJRS27 is specifically manufactured for optimum performance in Tetramer and Multimer applications as well as Flow Cytometry and bead-based assays such as the Luminex® platform.

Size:  1 mg, 10 mg

Product Code: PJRS27

FAQs

Q. Which Streptavidin-RPE and Streptavidin-APC conjugates should I use in a tetramer application?

Streptavidin-RPE:

We suggest PJRS25, which is used in the protocol developed by the NIH tetramer core facility at Emory.

http://tetramer.yerkes.emory.edu/support/protocols#10

PJRS27 may also be an option. We suggest staying away from larger conjugates such as PJRS34.

Streptavidin-APC:

PJ27S Streptavidin-APC is used in the Emory tetramer protocol.

http://tetramer.yerkes.emory.edu/support/protocols#10

We do not suggest using PJ25S, this is a large APC conjugate which may not be optimal for this application. PJ25S is larger than PJ27S and may give higher signal but may also have higher background.

Q. ProZyme has a number of Streptavidin-R-Phycoerythrin conjugates. How are they different and which one should I use?

A. Our range of Streptavidin-R-Phycoerythrin (SA-RPE) conjugates differ in overall size. We recommend that you test our conjugates to find out which is the best for your system. 

Please contact us to set up a sample to test in your application or order the SA-RPE sampler kit, PJ3SX, that contains the following conjugates (0.25mg ea): PJRS20PJRS301PJRS34PJRS25PJRS27PJ39S

 Product Size Application

PJRS301†  PJRS20  PJRS34

Large Luminex and other bead based assays  

PJRS25†† PJRS27

Medium Tetramer
PJ39S Small Flow cytometry

 PJRS301 is our newest conjugate, developed for long-term signal consistency.

PJRS25 is used in the NIH (Emory) Tetramer Core Facility protocol published online, along with our streptavidin-APC (allophycocyanin) conjugate PJ27S. 

Q. What dilution should I use for your SA-RPE conjugates?

A. We don’t have set protocols for our SA-RPE conjugates, as this may depend on your system & application. Please contact us if you are interested in a sample to test.

As a frame of reference, for our SA-RPE Luminex QC assay we dilute to 10 μg/ml and add 25 μl (0.25 μg) per well. Dilution of SA-RPE to 2-20 μg/ml for addition to the wells is a good starting point for Luminex.

Safety

Product Safety Documentation for PJRS27:

Product/Part No. Description
PJRS27 Streptavidin-R-Phycoerythrin