A conjugate that is predominantly a single peak on an HPLC chromatogram, consistent with approximately two streptavidin molecules conjugated to a single R-phycoerythrin molecule (conjugate MW ~ 295 kDa).
Potential applications include single molecule detection systems and quantitative analysis in flow cytometry.
Size: 1 mg
Product Code: PJ39S
Q. What happened to PJRS22, has it been replaced by PJ39S?
A. Yes, PJRS22 was discontinued, the replacement is PJ39S.
Similarly to PJRS22, PJ39S is also predominantly a single peak on an HPLC-SEC chromatogram, consistent with approximately two streptavidin molecules conjugated to a single R-phycoerythrin molecule. Performance of PJRS22 and PJ39S should be comparable.
Q. ProZyme has a number of Streptavidin-R-Phycoerythrin conjugates. How are they different and which one should I use?
A. Our range of Streptavidin-R-Phycoerythrin (SA-RPE) conjugates differ in overall size. We recommend that you test our conjugates to find out which is the best for your system.
Please contact us to set up a sample to test in your application or order the SA-RPE sampler kit, PJ3SX, that contains the following conjugates (0.25mg ea): PJRS20, PJRS301, PJRS34, PJRS25, PJRS27, PJ39S
|Large||Luminex and other bead based assays|
†PJRS301 is our newest conjugate, developed for long-term signal consistency.
Q. What dilution should I use for your SA-RPE conjugates?
A. We don’t have set protocols for our SA-RPE conjugates, as this may depend on your system & application. Please contact us if you are interested in a sample to test.
As a frame of reference, for our SA-RPE Luminex QC assay we dilute to 10 μg/ml and add 25 μl (0.25 μg) per well. Dilution of SA-RPE to 2-20 μg/ml for addition to the wells is a good starting point for Luminex.
Product Safety Documentation for PJ39S:
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