Streptavidin-Allophycocyanin [PJ27S]

Price:
$ 75.00

Description

PhycoLink® Streptavidin-Allophycocyanin

ProZyme® PhycoPro™ GT5™ APC (Spirulina platensis) was cross-linked and conjugated to Streptavidin (Streptomyces avidinii). This product is specifically designed for use in tetramer and multimer applications.

Size: 0.25 mg, 1 mg

Product Code: PJ27S

FAQs

Q. Which Streptavidin-RPE and Streptavidin-APC conjugates should I use in a tetramer application?

Streptavidin-RPE:

We suggest PJRS25, which is used in the protocol developed by the NIH tetramer core facility at Emory.

http://tetramer.yerkes.emory.edu/support/protocols#10

PJRS27 may also be an option. We suggest staying away from larger conjugates such as PJRS34.

Streptavidin-APC:

PJ27S Streptavidin-APC is used in the Emory tetramer protocol.

http://tetramer.yerkes.emory.edu/support/protocols#10

We do not suggest using PJ25S, this is a larger APC conjugate which may not be optimal for this application.  PJ25S is larger than PJ27S and may give higher signal but may also have higher background.

Product Citations
  1. NIH Tetramer Core Facility. [Internet]. Atlanta, GA: Emory University; c2006-2010. Class I MHC Tetramer Preparation: Overview; [cited 2016 Mar 23].  Available from: http://tetramer.yerkes.emory.edu/support/protocols 
  2. Leonard JD, Gilmore DC, Dileepan T, Nawrocka WI, Chao JL, Schoenbach MH, Jenkins MK, Adams EJ, Savage PA.  Identification of Natural Regulatory T Cell Epitopes Reveals Convergence on a Dominant Autoantigen.   2017 Jul 18;47(1):107-117.e8. doi: 10.1016/j.immuni.2017.06.015. Epub 2017 Jul 11.
Safety

Product Safety Documentation for PJ27S:

Product/Part No. Description
PJ27S Streptavidin-Allophycocyanin