Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans [GK80110]

Price:
$ 359.00

Description

(formerly GE50)

Contains all enzymes & reagents needed to remove all N-linked glycans & simple O-linked glycans (including polysialylated) from glycoproteins. See the product manual for an example using bovine fetuin.

N-Glycanase (PNGase F) cleaves all N-linked complex, hybrid or high mannose oligosaccharides unless α(1,3) core fucosylated (asparagine must be peptide-bonded at both termini).

Unfortunately there is no such “universal” endoglycosidase for O-glycans, these have to be trimmed down to Gal-GalNAc-O-S/T before O-glycanase (GK80090) can remove the Gal-GalNAc structure. Modification of this core with e.g. sialic acid will block O-glycanase.

Sialidase A (GK80040) is included in the kit and can remove terminal sialic acid from sialylated T antigen/Core 1 (Sia-Gal-GalNAc-O-S/T) to reveal core 1 Gal-GalNAc. The GK80115 extender kit (β(1,4)Galactosidase & β-N-Acetylglucosaminidase), available separately, can assist with removal of e.g. core 2 structures (GlcNAc-Gal-GalNAc-S/T). However, for more extended & complex O-glycans structures these enzymes will not be sufficient to reveal Gal-GalNAc. For more on O-GalNAc glycan core structures, please see reference [1].

Size: 1 kit (~2 mg of glycoprotein)

Product Code: GK80110

1. , , and . Essentials of Glycobiology. 2nd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009. Chapter 29, O-GalNAc Glycans.

Tech Info

GK80110 Instruction Manual  

Product Citations
  1. Matsuda C, Shiota Y, Sheikh AM, Okazaki R, Yamada K, Yano S, Minohata T, Matsumoto KI, Yamaguchi S, Nagai A. Quantification of CSF cystatin C using liquid chromatography tandem mass spectrometry.  2018 Mar;478:1-6
Safety

Product Safety Documentation for GK80110:

Product/Part No. Description
GK80110 GlycoPro™ Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans 
WS0021

US-Bovine Fetuin Control 

GB-Bovine Fetuin Control 

WS0041 N-Glycanase® PNGase F
WS0042 Sialidase A™
WS0043 O-Glycanase™
WS0046 5x Reaction Buffer 
WS0056 Denaturation Solution 
WS0057 Detergent Solution