Releases non-reducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Supplied at a higher concentration than GKX-5003. Higher enzyme concentrations may be necessary to remove bisecting β(1-4)-linked GlcNAc residues in complex N-glycans.
The enzyme exhibits a broad specificity, cleaving non-reducing terminal β(1-2,3,4, and 6)-linked-N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. It has proved very useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with β-N-Acetylhexosaminidase from S. Pneumoniae (GK80050).
Figure 1: Cleavage specificity for GKX-5023 β N-Acetylhexosaminidase.
1 ea WS0055 5x Enzyme Reaction Buffer (S6) (500mM sodium phosphate, pH 5.5)
20mM sodium citrate phosphate (pH 6.0)
5x Enzyme Reaction Buffer (S6) (500mM sodium phosphate, pH 5.5)
~67 kD (from literature)
One unit of β-N-Acetylhexosaminidase is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from pNP-N-acetyl-β-D-glucosaminide per minute at pH 5.0 and 37°C.
Size: 15 U in 50µl
Concentration: ≥ 300 U/ml
Product Code: GKX-5023
Product Safety Documentation for GKX-5023:
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