Releases α(1-2)-linked mannose residues from the non-reducing terminus of oligosaccharides. Used in exoglycosidase sequencing.
The enzyme specifically cleaves α(1-2)-linked mannose from the non-reducing terminus of glycans. As such it is extremely valuable in identifying this type of linkage and, in particular, the presence of high mannose-type N-glycans which are digested to the Man-5 structure.
Figure 1: Cleavage specificity for GKX-5009 α(1-2)-Mannosidase.
WS0120 5x Reaction Buffer (500 mM sodium acetate pH 5.0)
5X concentrated buffer which when diluted gives 100 mM sodium acetate pH 5.0.
Lyophilized from water containing 250 μg/ml bovine serum albumin.
MW: ~51 kD
pH optimum/range: 5.0
One Unit is defined as the amount of enzyme that will release 1 μmole of mannose from methyl-2-O-α-d-mannopyranosyl-α-d-mannopyranoside per minute at pH 5.0 and 37°C.
2 mU is equal to 50μU as previously reported by Glyko, Inc.
Size: 2 mU lyophilized
Product Code: GKX-5009
Q. Can I use α(1-2)-Mannosidase to cleave phosphorylated mannose?
A. Information in the literature suggests that the action of α(1-2)-Mannosidase is blocked by phosphorylated mannose .
1. Kim MW, Rhee SK, Kim JY, Shimma Y, Chiba Y, Jigami Y, Kang HA. Glycobiology. 2004 Mar;14(3):243-51. Epub 2003 Dec 23.
Product Safety Documentation for GKX-5009:
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