Cleaves within the N,N'-diacetylchitobiose (GlcNAc-GlcNAc) core of high mannose and some hybrid type N-glycans. Endoglycosidase H, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase.
Recombinant gene from Streptomyces plicatus1, expressed in E. coli.
Cleaves between the GlcNAc residues of the chitobiose (GlcNAc-GlcNAc) core of N-linked glycans. Oligomannose & some hybrid type N-glycans are released from glycoproteins, leaving a single GlcNAc attached to asparagine. This specificity is distinct from Endo F2 (GKE-5008) which also cleaves biantennary complex type N-glycans, and PNGase F (GKE-5006) which releases most N-glycans by cleaving between the innermost GlcNAc and asparagine.
Figure 1: Cleavage specificity for GKE-5002 Endo H.
R2: H, monosaccharide or oligosaccharide at C2 and/or C4
R3: GlcNAc ± Fuc, Asn or Dol-pp
Selective release of high mannose N-linked oligosaccharides from glycopeptides/ glycoproteins. The enzyme has found extensive use in the characterization of glycoproteins, dolichol-linked glycans and the biosynthetic pathway for N-glycosylation.
50 mM sodium phosphate pH 7.5, 25 mM EDTA, and 0.05% sodium azide
One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 60 μmoles of denatured ribonuclease B in 1 hour at 37°C, pH 5.5.
Size: 30 mU (200 µl)
Concentration: ≥ 0.15 U/ml
Product Code: GKE-5002
1. Robbins PW, Trimble RB, Wirth DF, Hering C, Maley F, Maley GF, Das R, Gibson BW, Royal N, Biemann K. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosaminidase H. J Biol Chem. 1984 Jun 25;259(12):7577-83.
EC Number: 126.96.36.199
CAS Number: 37278-88-9
Product Safety Documentation for GKE-5002:
|GKE-5002||Glyko® Endo H|||
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