The new Gly-X with InstantPC kits combine a 5-minute in-solution enzymatic protein deglycosylation step followed by 1-minute labeling of released N-glycans with InstantPC dye. A simple and efficient clean-up step removes dye and other interfering compounds. Labeled glycans are ready for analysis by UHPLC, LC-MS, MS/MS. The Gly-X sample prep method is simple and rapid. InstantPC dye delivers unmatched fluorescent brightness, its small size contributes to improved HILIC resolution and a tertiary amine provides increased MS signal in positive mode for N-glycan identification.
Gly-X with InstantPC kits:
For more information, please contact us.
We were pleased to present a poster on our InstantPC glycan labeling dye at ASMS 2016 in San Antonio (June 5-9). The work utilized the GPPNG-PC kit, GlykoPrep-plus with InstantPC labeling, automated on the Agilent AssayMAP Bravo.
A handout version of our ASMS 2016 poster is available here:
We are pleased to announce the launch of 3 new GlykoPrep kits for automated N-glycan sample preparation on the Agilent AssayMAP Bravo.
- GPPNG-PC, GlykoPrep-plus Rapid N-Glycan Sample Preparation with InstantPC is for the preparation of released, InstantPC-labeled N-glycans for separation by liquid chromatography with fluorescence detection and mass spectrometry. This new dye was specifically engineered for LC/MS analysis and provides exceptional response in both fluorescence and mass spectrometry applications. Instant labeling allows for an automated workflow of around 3 hours!
- GPPNG-LB, GlykoPrep-plus Rapid N-Glycan Sample Preparation with InstantAB is for the preparation of released, InstantAB- labeled N-glycans for separation by liquid chromatography with fluorescence detection. Instant labeling allows for an automated workflow of around 3 hours!
- GPPNG-APTS, GlykoPrep-plus Rapid N-Glycan Sample Preparation with APTS (8-
These 3 new products are in addition to our existing GlykoPrep-plus 2-AB kit, GPPNG-AB. InstantPC, InstantAB, 2-AB and APTS GlykoPrep-plus kits are provided in a 96-count format with sufficient reagents to prepare 96 labeled N-glycan samples, in a single Bravo run or in 4 runs totaling 96 replicates.
For more information, please contact us.
We are proud to announce that we are now an ISO 9001:2008 company. ISO certification is another milestone in our history and continuation of our commitment to excellence.
ProZyme's achievement demonstrates a robust quality management system that guides and governs our design, development, manufacture, distribution and support of our products.
This certification is an important step in fulfilling our mission of delivering innovative products to our biopharma, diagnostics and academic customers worldwide.
Welcome to our new website! Redesigned www.prozyme.com provides 24/7 access to product and application information to our customers and partners. The site is built on a modern e-commerce platform that enables online checkouts in the USA and supports traditional quotation/ordering workflows for international customers. Immediately you will notice streamlined menus, simple navigation and access to the information you need. You will see product pages that have product images and tabs for product description, technical information and certificates of analysis. We started two blogs: News and Events & Tradeshows. An optional Newsletter sign up can keep you up to date with the latest developments at ProZyme!
ProZyme, Inc., manufacturer of bioreagents for glycobiology, and BiOptic Inc. of Taiwan, manufacturer of instruments for DNA analysis by capillary gel electrophoresis (CGE), today announce an agreement to collaborate on the development and commercialization of an integrated system (Gly-Q™) for high-throughput, user-friendly glycoanalysis. The Gly-Q collaboration will extend ProZyme’s portfolio of glycobiology products, and will leverage BiOptic’s line of rugged and accurate CGE instruments.
Under the terms of the OEM/ODM licensing agreement, ProZyme will utilize BiOptic’s automated CGE platform with patented fluorescence detection and replaceable gel-cartridge design for rapid separation and detection of labeled glycans. At the front end of the Gly-Q system, enzymatically-released and fluorescently-labeled glycans will be prepared using ProZyme chemistry prior to glycan separation using the automated CGE instrument. Data analysis will be simplified by the use of customized software and ProZyme N-glycan reference standards. The combined Gly-Q system for N-glycan sample preparation, separation and analysis will be exclusively marketed by ProZyme worldwide.
Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies. Traditional methods for glycan analysis involve slow and labor-intensive steps for sample preparation, glycan separation and data analysis. To this end, ProZyme and BiOptic plan to introduce the Gly-Q system to enable high-throughput and accessible glycoanalysis for use in applications such as biotherapeutic cell line development.
ProZyme will present preliminary data on use of the Gly-Q system for N-glycan analysis of biotherapeutics in a poster presentation at the California Separation Science Society (CASSS) CE Pharm meeting held in Brooklyn, NY, September 20-24, 2015. The commercialized ProZyme Gly-Q glycoanalysis workflow is expected to be available in early 2016.
BusinessWire Press Release