Important Ordering Information for ProZyme-Agilent Transition - How your current ordering process will change when ProZyme becomes Agilent on August 5, 2019.
ProZyme Becomes Agilent Technologies on August 5
In 2018, Agilent completed the acquisition of ProZyme, Inc., a leading provider glycobiology portfolio, alongside streptavidin, phycobiliprotein and conjugate offerings. With the addition of ProZyme products and services, Agilent provides a single-source offering for instruments and consumables, from sample to trusted answer. Our expertise now covers the complete glycan analysis workflow, in addition to other critical quality attributes, so you can easily get the reliable, reproducible results you need – fast. Learn more how we are better together.
We are now completing the system integration and have important information for you on this transition. Important changes will take effect on August 5, 2019.
You may have received an email notification of these upcoming changes, the letter is available here:
FREQUENTLY ASKED QUESTIONS
ORDERING PROZYME PRODUCTS
I have a current quote, is it still valid?
Yes, all current quotes will be honored through their expiration date.
How do I place an order for ProZyme products?
Please note that until August 5, 2019, there will be no change to your current ordering process with ProZyme. In addition, any deliveries in transit that correspond to orders placed by your organization before August 5, will be fulfilled by ProZyme.
From August 5, your organization will no longer be able to place new orders with ProZyme and should place orders directly with Agilent. Please update your vendor records to be effective on August 5, with the new Agilent information on the country-specific Fact Sheet.
Will ProZyme product numbers be changing?
Existing ProZyme numbers are not changing. However, additional new pack size specific part numbers are being introduced for limited set of products on August 5. Please contact your local sales rep for more info or contact your local Agilent technical support Agilent.com Contact Us
Will order payments still be made to ProZyme?
If you have an invoice from ProZyme for a recent order, you should make the payment to ProZyme per the instructions on that invoice.
As of August 5, 2019, all new orders will be fulfilled by Agilent, and invoices will be sent from Agilent with remittance instructions on the invoice. You can also refer to the country-specific Fact Sheet for additional details.
I am a customer who is already set-up with Agilent as a vendor, do I need to do or change anything to my current vendor information?
No, if you are already set-up with Agilent as a vendor you do not need to do anything. You can also refer to the country-specific Fact Sheet for additional details.
Who do I contact for technical support related to ProZyme products?
Until August 5, 2019, you can continue to contact ProZyme technical support representatives by phone at 1-800-457-9444 or 1-510-638-6900, or email firstname.lastname@example.org. Representatives are available from 8:00 a.m. to 5:00 p.m. Pacific Time, Monday through Friday, to answer your questions.
Technical support for ProZyme Europe customers is available at phone number +45 44 20 98 74, from 9:00am to 6:00pm CET, Monday through Friday.
On August 5, Technical Support contact numbers will change, with details available on Agilent.com Contact Us.
Will my ProZyme contact numbers remain the same?
Through August 4, all ProZyme contact numbers available on www.ProZyme.com will remain the same. On August 5, there will be country-specific changes, with details available on Agilent.com Contact Us.
Are ProZyme emails changing?
Yes, ProZyme email addresses have changed to Agilent email addresses. For example: bsmith@ProZyme.com is now email@example.com. You may continue to use the ProZyme email, which will forward to the new Agilent email, but we recommend you update your records to reflect the new Agilent email address.
How do I schedule technical training?
To schedule technical training, please contact your sales representative or product specialist, who will be able to advise you on current technical training available.
Will I continue to receive ProZyme emails/notifications?
Yes, if you are currently subscribed, you will continue to receive all emails/notifications. Should you wish to subscribe, visit the website to Register.
Several of us from ProZyme are attending the annual ASMS conference this week in Atlanta, GA. As always, it's a great opportunity to learn about the latest advances in the mass spec field, particularly as it relates to glycan analysis, and to interact with its practitioners in academia and industry.
We're also pleased to be interacting with Agilent at this conference as part of our campaign to advance glycosciences, together.
Today (Wednesday), John Yan presents poster 228 in the Clinical Analysis III session, on behalf of our collaborator Sarah Totten of Stanford University School of Medicine. The poster, "Quantitative N-Glycan Profiling of Clinical Tissue Samples by On-Line Fluorescence-MS Using a Rapid Labeling Tag", is a collaboration led by the Canary Center at Stanford for Early Cancer Detection. The work examines proteins extracted from prostate cancer tissue, and utilizes Gly-X with InstantPC sample preparation to obtain the released N-glycan profile from glycopeptides in conjunction with Agilent 1260 LC and Agilent 6230 TOF systems.
On Thursday, Aled Jones will present poster 697, "Streamlined Workflows for N-Glycan Analysis of Biotherapuetics Using InstantPC and 2-AB with LC-FLD-MS" as part of the Proteins: PTMs II session. The poster examines the released N-glycan profiles obtained for rituximab (mAb) and etanercept (Fc fusion) using our Gly-X InstantPC and 2-AB Express kits, with separation on Agilent 1290 Infinity II UHPLC and AdvanceBIo Glycan Mapping columns. Fluorescence and mass spec detection of labeled N-glycans are with the Agilent 1260 Infinity II FLD and Agilent 6545 LC/QTOF MS, respectively.
On August 1st, Agilent completed the acquisition of ProZyme. ProZyme provides a greater scale to Agilent's biopharma consumables business and enhances Agilent's value proposition by providing our customers a complete glycan analysis workflow solution.
Below are answers to common questions about ProZyme and Agilent.
Q: Who do I contact to order ProZyme products, or request product information?
Agilent cannot process any orders for ProZyme products until the acquisition is closed and integration is completed. For now, please visit the ProZyme website for guidance on ordering directly or through ProZyme's distributor network, and for product information.
Q: Who do I contact for technical support related to ProZyme products?
You can continue to contact ProZyme technical support representatives at 1 (800) 457-9444 or email firstname.lastname@example.org. We are available to answer your questions from 9.00am to 6.00pm Pacific Time, Monday through Friday.
Technical support for ProZyme Europe customers is available at +45 44 20 98 74, from 9.00am to 6.00pm CET, Monday through Friday.
Q: Can Agilent customers include ProZyme products on their Agilent orders?
For now, all orders for ProZyme products must be handled through ProZyme's distributor network and website, until the acquisition is closed and integration is completed.
Q: Will part numbers stay the same?
For now, part numbers for ProZyme products will stay the same.
We are proud to announce that we are now an ISO 9001:2015 company. ISO certification is another milestone in our history and continuation of our commitment to excellence.
ProZyme's achievement demonstrates a robust quality management system that guides and governs our design, development, manufacture, distribution and support of our products.
This certification is an important step in fulfilling our mission of delivering innovative products to our biopharma, diagnostics and academic customers worldwide.
N-Glycans from Enbrel released and labeled with Gly-X 2-AB Express, and separated by hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.
HAYWARD, Calif., June 6, 2017 /PRNewswire-iReach/ -- ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-X™ N-Glycan Rapid Release and Labeling with 2-AB Express™ and Gly-X™ N-Glycan Rapid Release and Labeling with InstantAB™ kits. Both kits leverage ProZyme's next generation Gly-X™ platform that provides a simplified in-solution workflow for N-glycan analysis. The innovative 2-AB Express™ labeling method shortens sample preparation time to 2.5 hours, while the industry leading InstantDye™ labeling allows for even shorter 1.5-hour preparation time. The launch of these two new innovative products expands ProZyme's Gly-X product portfolio that now includes 2-AB Express, InstantAB, InstantPC™ and InstantQ™ dyes for LC-FLR, LC/MS and CE applications.
"We are pleased to introduce two additions to ProZyme's Gly-X offering," said Sergey Vlasenko, Ph.D., President and CEO of ProZyme. "We developed the Gly-X platform to speed up glycan sample preparation, but two very commonly used labels, 2-AB and InstantAB, have been missing. With the launch of the Gly-X 2-AB Express and Gly-X InstantAB kits, analytical and QC groups at biopharma companies can simplify their processes and get the N-glycan results faster. In addition, the use of well-established labels will assure data continuity and enable easier platform transition for ongoing projects. I believe the next generation Gly-X platform will deliver real improvements to the way N-glycan analysis is performed today."
Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies, and N-glycosylation can be a critical quality attribute. Protein glycosylation can also serve as a signature of a specific disease state. Traditional methods for N-glycan analysis involve slow and labor-intensive steps for sample preparation. A fluorophore is frequently added to released glycans to aid detection, and 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years. Typically, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling.
ProZyme Gly-X chemistry enzymatically releases N-glycans from glycoproteins with PNGase F in 5 minutes, and released N-glycans are labeled with a fluorescent dye instantly (InstantAB, InstantPC or InstantQ), or via on-matrix 1 hour reductive amination reaction with no prior dry down (2-AB Express). The entire sample preparation protocol can be completed in less than 1.5 hours for InstantDye or 2.5 hours for reductive amination dye. The protocol can be automated on common laboratory liquid handlers.
ProZyme supports Gly-X sample preparation workflows with a range of labeled N-glycan standards. The Gly-X™ 2-AB Express, InstantAB, InstantPC and InstantQ kits are exclusively marketed by ProZyme worldwide.
To learn more about the Gly-X N-glycan sample preparation system, please visit prozyme.com:
ProZyme, Inc. is a privately held company based in Hayward CA founded in 1990 with a vision of establishing a high-quality, value-added and customer-driven biochemical reagent company. We are proud, 27 years on, to provide the highest level of products, services and support to our customers at biotechnology, biopharmaceutical and academic institutions across the world. ProZyme's flagship product line is an extensive glycobiology portfolio, alongside streptavidin, phycobiliprotein and conjugate offerings. We perform custom analytical services, and welcome the opportunity to collaborate with our customers. ProZyme maintains a commitment to develop, release and support products in the exciting and rapidly-expanding area of glycobiology.
ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-Q™ Glycan Analysis System, an integrated platform for high-throughput, user-friendly glycoanalysis. The Gly-Q System will leverage ProZyme's expertise in rapid glycan sample preparation and analytical glycan reference standards, combined with a small capillary gel electrophoresis (CGE) instrument powered by custom software for simplified glycan data analysis.
"We are delighted to introduce ProZyme's first instrument offering," said Sergey Vlasenko, Ph.D., President and CEO of ProZyme. "We have led the way in speeding up glycan sample preparation in the past few years, but data analysis has still been a bottleneck. With our new system, cell line development and analytical groups at biopharma companies can determine the glycan profiles of their samples faster, while reducing overall costs. I believe this system could benefit researchers running glycomic analysis of large datasets including clinical samples."
Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies, and N-glycosylation can be a critical quality attribute. Protein glycosylation can also serve as a signature of a specific disease state. Traditional methods for N-glycan analysis involve slow and labor-intensive steps for sample preparation, glycan separation and data analysis. ProZyme introduces the Gly-Q system to enable high-throughput and accessible glycan analysis for use in biotherapeutic development, and to aid in glycan biomarker discovery.
At the front end of the Gly-Q workflow, ProZyme Gly-X chemistry enzymatically releases N-glycans from glycoproteins with PNGase F in 5 minutes, and released N-glycans are instantly labeled with a fluorescent dye that enables separation by CGE. The entire sample preparation protocol can be completed in as little as 45 minutes, and is automatable. Fluorescently-labeled N-glycans are then separated on the Gly-Q CGE instrument with a run time of 2 minutes per sample, allowing 96 samples to be prepared and analyzed within a single 8-hour work day. The instrument features an easily-replaceable Gly-Q cartridge containing a capillary and gel matrix.
Gly-Q instrument control and N-glycan data analysis is facilitated by custom-built Gly-Q Manager software. Migration standards are co-injected with each sample, which along with a ladder standard enables automatic data alignment and the calculation of glucose unit (GU) values for each sample peak. GU values for samples are then compared against built-in and customizable N-glycan reference libraries for peak assignment. Analysis is enhanced with ProZyme's glycan reference standards and exoglycosidase enzymes.
The combined Gly-Q System for N-glycan sample preparation, separation and analysis is exclusively marketed by ProZyme worldwide.