N-Glycans from Enbrel released and labeled with Gly-X 2-AB Express, and separated by hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.
HAYWARD, Calif., June 6, 2017 /PRNewswire-iReach/ -- ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-X™ N-Glycan Rapid Release and Labeling with 2-AB Express™ and Gly-X™ N-Glycan Rapid Release and Labeling with InstantAB™ kits. Both kits leverage ProZyme's next generation Gly-X™ platform that provides a simplified in-solution workflow for N-glycan analysis. The innovative 2-AB Express™ labeling method shortens sample preparation time to 2.5 hours, while the industry leading InstantDye™ labeling allows for even shorter 1.5-hour preparation time. The launch of these two new innovative products expands ProZyme's Gly-X product portfolio that now includes 2-AB Express, InstantAB, InstantPC™ and InstantQ™ dyes for LC-FLR, LC/MS and CE applications.
"We are pleased to introduce two additions to ProZyme's Gly-X offering," said Sergey Vlasenko, Ph.D., President and CEO of ProZyme. "We developed the Gly-X platform to speed up glycan sample preparation, but two very commonly used labels, 2-AB and InstantAB, have been missing. With the launch of the Gly-X 2-AB Express and Gly-X InstantAB kits, analytical and QC groups at biopharma companies can simplify their processes and get the N-glycan results faster. In addition, the use of well-established labels will assure data continuity and enable easier platform transition for ongoing projects. I believe the next generation Gly-X platform will deliver real improvements to the way N-glycan analysis is performed today."
Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies, and N-glycosylation can be a critical quality attribute. Protein glycosylation can also serve as a signature of a specific disease state. Traditional methods for N-glycan analysis involve slow and labor-intensive steps for sample preparation. A fluorophore is frequently added to released glycans to aid detection, and 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years. Typically, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling.
ProZyme Gly-X chemistry enzymatically releases N-glycans from glycoproteins with PNGase F in 5 minutes, and released N-glycans are labeled with a fluorescent dye instantly (InstantAB, InstantPC or InstantQ), or via on-matrix 1 hour reductive amination reaction with no prior dry down (2-AB Express). The entire sample preparation protocol can be completed in less than 1.5 hours for InstantDye or 2.5 hours for reductive amination dye. The protocol can be automated on common laboratory liquid handlers.
ProZyme supports Gly-X sample preparation workflows with a range of labeled N-glycan standards. The Gly-X™ 2-AB Express, InstantAB, InstantPC and InstantQ kits are exclusively marketed by ProZyme worldwide.
To learn more about the Gly-X N-glycan sample preparation system, please visit prozyme.com:
ProZyme, Inc. is a privately held company based in Hayward CA founded in 1990 with a vision of establishing a high-quality, value-added and customer-driven biochemical reagent company. We are proud, 27 years on, to provide the highest level of products, services and support to our customers at biotechnology, biopharmaceutical and academic institutions across the world. ProZyme's flagship product line is an extensive glycobiology portfolio, alongside streptavidin, phycobiliprotein and conjugate offerings. We perform custom analytical services, and welcome the opportunity to collaborate with our customers. ProZyme maintains a commitment to develop, release and support products in the exciting and rapidly-expanding area of glycobiology.