We were pleased to present a poster on our Gly-X with InstantPC kit at the 28th International Carbohydrate Symposium.
The new Gly-X with InstantPC kit combines a 5-minute in-solution enzymatic protein deglycosylation step followed by 1-minute labeling of released N-glycans with InstantPC dye. A simple and efficient clean-up step removes dye and other interfering compounds. Labeled glycans are ready for analysis by UHPLC, LC-MS, MS/MS. The Gly-X sample prep method is simple and rapid. InstantPC dye delivers unmatched fluorescent brightness, its small size contributes to improved HILIC resolution and a tertiary amine provides increased MS signal in positive mode for N-glycan identification.
A handout version of our ICS 2016 poster is available here:
Development of a 5-Minute Deglycosylation Method and Instant Labeling Dye for High Throughput N-Glycan Analysis by Mass Spectrometry
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