ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-Q™ Glycan Analysis System, an integrated platform for high-throughput, user-friendly glycoanalysis. The Gly-Q System will leverage ProZyme's expertise in rapid glycan sample preparation and analytical glycan reference standards, combined with a small capillary gel electrophoresis (CGE) instrument powered by custom software for simplified glycan data analysis.
"We are delighted to introduce ProZyme's first instrument offering," said Sergey Vlasenko, Ph.D., President and CEO of ProZyme. "We have led the way in speeding up glycan sample preparation in the past few years, but data analysis has still been a bottleneck. With our new system, cell line development and analytical groups at biopharma companies can determine the glycan profiles of their samples faster, while reducing overall costs. I believe this system could benefit researchers running glycomic analysis of large datasets including clinical samples."
Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies, and N-glycosylation can be a critical quality attribute. Protein glycosylation can also serve as a signature of a specific disease state. Traditional methods for N-glycan analysis involve slow and labor-intensive steps for sample preparation, glycan separation and data analysis. ProZyme introduces the Gly-Q system to enable high-throughput and accessible glycan analysis for use in biotherapeutic development, and to aid in glycan biomarker discovery.
At the front end of the Gly-Q workflow, ProZyme Gly-X chemistry enzymatically releases N-glycans from glycoproteins with PNGase F in 5 minutes, and released N-glycans are instantly labeled with a fluorescent dye that enables separation by CGE. The entire sample preparation protocol can be completed in as little as 45 minutes, and is automatable. Fluorescently-labeled N-glycans are then separated on the Gly-Q CGE instrument with a run time of 2 minutes per sample, allowing 96 samples to be prepared and analyzed within a single 8-hour work day. The instrument features an easily-replaceable Gly-Q cartridge containing a capillary and gel matrix.
Gly-Q instrument control and N-glycan data analysis is facilitated by custom-built Gly-Q Manager software. Migration standards are co-injected with each sample, which along with a ladder standard enables automatic data alignment and the calculation of glucose unit (GU) values for each sample peak. GU values for samples are then compared against built-in and customizable N-glycan reference libraries for peak assignment. Analysis is enhanced with ProZyme's glycan reference standards and exoglycosidase enzymes.
The combined Gly-Q System for N-glycan sample preparation, separation and analysis is exclusively marketed by ProZyme worldwide.
We were pleased to present a poster on our new Gly-Q Glycan Analysis System at the annual Bioprocessing Summit.
The poster highlighted a complete glycan analysis solution for rapid sample preparation and analysis combined with a simplified data processing approach. The sample preparation included a 5-minute enzymatic deglycosylation step to release N-glycans, followed by 1-minute glycan labeling with InstantQ dye. After a simple and effecient clean-up step, labeled N-glycans were separated on Gly-Q system, a small user-friendly capillary electrophoresis (CE) instrument, with a run time of 2 minutes per sample.
A handout version of our 2016 Bioprocessing Summit poster is available here:
An Integrated Solution for High-throughput, User-friendly Glycoanalysis Using Rapid Separation by Capillary Electrophoresis
We were pleased to present a poster on our Gly-X with InstantPC kit at the 28th International Carbohydrate Symposium.
The new Gly-X with InstantPC kit combines a 5-minute in-solution enzymatic protein deglycosylation step followed by 1-minute labeling of released N-glycans with InstantPC dye. A simple and efficient clean-up step removes dye and other interfering compounds. Labeled glycans are ready for analysis by UHPLC, LC-MS, MS/MS. The Gly-X sample prep method is simple and rapid. InstantPC dye delivers unmatched fluorescent brightness, its small size contributes to improved HILIC resolution and a tertiary amine provides increased MS signal in positive mode for N-glycan identification.
A handout version of our ICS 2016 poster is available here:
Development of a 5-Minute Deglycosylation Method and Instant Labeling Dye for High Throughput N-Glycan Analysis by Mass Spectrometry
The new Gly-X with InstantPC kits combine a 5-minute in-solution enzymatic protein deglycosylation step followed by 1-minute labeling of released N-glycans with InstantPC dye. A simple and efficient clean-up step removes dye and other interfering compounds. Labeled glycans are ready for analysis by UHPLC, LC-MS, MS/MS. The Gly-X sample prep method is simple and rapid. InstantPC dye delivers unmatched fluorescent brightness, its small size contributes to improved HILIC resolution and a tertiary amine provides increased MS signal in positive mode for N-glycan identification.
Gly-X with InstantPC kits:
For more information, please contact us.
We were pleased to present a poster on our InstantPC glycan labeling dye at ASMS 2016 in San Antonio (June 5-9). The work utilized the GPPNG-PC kit, GlykoPrep-plus with InstantPC labeling, automated on the Agilent AssayMAP Bravo.
A handout version of our ASMS 2016 poster is available here:
We are pleased to announce the launch of 3 new GlykoPrep kits for automated N-glycan sample preparation on the Agilent AssayMAP Bravo.
- GPPNG-PC, GlykoPrep-plus Rapid N-Glycan Sample Preparation with InstantPC is for the preparation of released, InstantPC-labeled N-glycans for separation by liquid chromatography with fluorescence detection and mass spectrometry. This new dye was specifically engineered for LC/MS analysis and provides exceptional response in both fluorescence and mass spectrometry applications. Instant labeling allows for an automated workflow of around 3 hours!
- GPPNG-LB, GlykoPrep-plus Rapid N-Glycan Sample Preparation with InstantAB is for the preparation of released, InstantAB- labeled N-glycans for separation by liquid chromatography with fluorescence detection. Instant labeling allows for an automated workflow of around 3 hours!
- GPPNG-APTS, GlykoPrep-plus Rapid N-Glycan Sample Preparation with APTS (8-
These 3 new products are in addition to our existing GlykoPrep-plus 2-AB kit, GPPNG-AB. InstantPC, InstantAB, 2-AB and APTS GlykoPrep-plus kits are provided in a 96-count format with sufficient reagents to prepare 96 labeled N-glycan samples, in a single Bravo run or in 4 runs totaling 96 replicates.
For more information, please contact us.
We are proud to announce that we are now an ISO 9001:2008 company. ISO certification is another milestone in our history and continuation of our commitment to excellence.
ProZyme's achievement demonstrates a robust quality management system that guides and governs our design, development, manufacture, distribution and support of our products.
This certification is an important step in fulfilling our mission of delivering innovative products to our biopharma, diagnostics and academic customers worldwide.