News

June 06, 2017

ProZyme, Inc. Launch Gly-X 2-AB Express and Gly-X InstantAB Kits for Streamlined N-Glycan Analysis

N-Glycans from Enbrel released and labeled with Gly-X 2-AB Express, and separated by hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.

HAYWARD, Calif., June 6, 2017 /PRNewswire-iReach/ -- ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-X™ N-Glycan Rapid Release and Labeling with 2-AB Express™ and Gly-X™ N-Glycan Rapid Release and Labeling with InstantAB™ kits. Both kits leverage ProZyme's next generation Gly-X™ platform that provides a simplified in-solution workflow for N-glycan analysis. The innovative 2-AB Express™ labeling method shortens sample preparation time to 2.5 hours, while the industry leading InstantDye™ labeling allows for even shorter 1.5-hour preparation time. The launch of these two new innovative products expands ProZyme's Gly-X product portfolio that now includes 2-AB Express, InstantAB, InstantPC™ and InstantQ™ dyes for LC-FLR, LC/MS and CE applications.       

"We are pleased to introduce two additions to ProZyme's Gly-X offering," said Sergey Vlasenko, Ph.D., President and CEO of ProZyme. "We developed the Gly-X platform to speed up glycan sample preparation, but two very commonly used labels, 2-AB and InstantAB, have been missing. With the launch of the Gly-X 2-AB Express and Gly-X InstantAB kits, analytical and QC groups at biopharma companies can simplify their processes and get the N-glycan results faster. In addition, the use of well-established labels will assure data continuity and enable easier platform transition for ongoing projects. I believe the next generation Gly-X platform will deliver real improvements to the way N-glycan analysis is performed today."

Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies, and N-glycosylation can be a critical quality attribute. Protein glycosylation can also serve as a signature of a specific disease state. Traditional methods for N-glycan analysis involve slow and labor-intensive steps for sample preparation. A fluorophore is frequently added to released glycans to aid detection, and 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years. Typically, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling.

ProZyme Gly-X chemistry enzymatically releases N-glycans from glycoproteins with PNGase F in 5 minutes, and released N-glycans are labeled with a fluorescent dye instantly (InstantAB, InstantPC or InstantQ), or via on-matrix 1 hour reductive amination reaction with no prior dry down (2-AB Express). The entire sample preparation protocol can be completed in less than 1.5 hours for InstantDye or 2.5 hours for reductive amination dye. The protocol can be automated on common laboratory liquid handlers. 

ProZyme supports Gly-X sample preparation workflows with a range of labeled N-glycan standards. The Gly-X™ 2-AB Express, InstantAB, InstantPC and InstantQ kits are exclusively marketed by ProZyme worldwide.

To learn more about the Gly-X N-glycan sample preparation system, please visit prozyme.com:

About ProZyme

ProZyme, Inc. is a privately held company based in Hayward CA founded in 1990 with a vision of establishing a high-quality, value-added and customer-driven biochemical reagent company. We are proud, 27 years on, to provide the highest level of products, services and support to our customers at biotechnology, biopharmaceutical and academic institutions across the world. ProZyme's flagship product line is an extensive glycobiology portfolio, alongside streptavidin, phycobiliprotein and conjugate offerings.  We perform custom analytical services, and welcome the opportunity to collaborate with our customers. ProZyme maintains a commitment to develop, release and support products in the exciting and rapidly-expanding area of glycobiology.

PR Newswire Press Release

November 14, 2016

ProZyme, Inc. Launch Gly-Q System for Integrated N-Glycan Analysis

ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-Q™ Glycan Analysis System, an integrated platform for high-throughput, user-friendly glycoanalysis. The Gly-Q System will leverage ProZyme's expertise in rapid glycan sample preparation and analytical glycan reference standards, combined with a small capillary gel electrophoresis (CGE) instrument powered by custom software for simplified glycan data analysis.

"We are delighted to introduce ProZyme's first instrument offering," said Sergey Vlasenko, Ph.D., President and CEO of ProZyme. "We have led the way in speeding up glycan sample preparation in the past few years, but data analysis has still been a bottleneck. With our new system, cell line development and analytical groups at biopharma companies can determine the glycan profiles of their samples faster, while reducing overall costs. I believe this system could benefit researchers running glycomic analysis of large datasets including clinical samples."

Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies, and N-glycosylation can be a critical quality attribute. Protein glycosylation can also serve as a signature of a specific disease state. Traditional methods for N-glycan analysis involve slow and labor-intensive steps for sample preparation, glycan separation and data analysis. ProZyme introduces the Gly-Q system to enable high-throughput and accessible glycan analysis for use in biotherapeutic development, and to aid in glycan biomarker discovery.

At the front end of the Gly-Q workflow, ProZyme Gly-X chemistry enzymatically releases N-glycans from glycoproteins with PNGase F in 5 minutes, and released N-glycans are instantly labeled with a fluorescent dye that enables separation by CGE. The entire sample preparation protocol can be completed in as little as 45 minutes, and is automatable. Fluorescently-labeled N-glycans are then separated on the Gly-Q CGE instrument with a run time of 2 minutes per sample, allowing 96 samples to be prepared and analyzed within a single 8-hour work day. The instrument features an easily-replaceable Gly-Q cartridge containing a capillary and gel matrix.

Gly-Q instrument control and N-glycan data analysis is facilitated by custom-built Gly-Q Manager software. Migration standards are co-injected with each sample, which along with a ladder standard enables automatic data alignment and the calculation of glucose unit (GU) values for each sample peak. GU values for samples are then compared against built-in and customizable N-glycan reference libraries for peak assignment. Analysis is enhanced with ProZyme's glycan reference standards and exoglycosidase enzymes.

The combined Gly-Q System for N-glycan sample preparation, separation and analysis is exclusively marketed by ProZyme worldwide.

PR Newswire Press Release

August 18, 2016

Gly-Q Glycan Analysis System Poster at the Bioprocessing Summit, Boston

We were pleased to present a poster on our new Gly-Q Glycan Analysis System at the annual Bioprocessing Summit.

The poster highlighted a complete glycan analysis solution for rapid sample preparation and analysis combined with a simplified data processing approach. The sample preparation included a 5-minute enzymatic deglycosylation step to release N-glycans, followed by 1-minute glycan labeling with InstantQ dye. After a simple and effecient clean-up step, labeled N-glycans were separated on Gly-Q system, a small user-friendly capillary electrophoresis (CE) instrument, with a run time of 2 minutes per sample. 

A handout version of our 2016 Bioprocessing Summit poster is available here:

An Integrated Solution for High-throughput, User-friendly Glycoanalysis Using Rapid Separation by Capillary Electrophoresis

 

July 25, 2016

Gly-X with InstantPC poster presented at 28th International Carbohydrate Symposium, New Orleans

We were pleased to present a poster on our Gly-X with InstantPC kit at the 28th International Carbohydrate Symposium.

The new Gly-X with InstantPC kit combines a 5-minute in-solution enzymatic protein deglycosylation step followed by 1-minute labeling of released N-glycans with InstantPC dye. A simple and efficient clean-up step removes dye and other interfering compounds. Labeled glycans are ready for analysis by UHPLC, LC-MS, MS/MS. The Gly-X sample prep method is simple and rapid. InstantPC dye delivers unmatched fluorescent brightness, its small size contributes to improved HILIC resolution and a tertiary amine provides increased MS signal in positive mode for N-glycan identification.

A handout version of our ICS 2016 poster is available here:

Development of a 5-Minute Deglycosylation Method and Instant Labeling Dye for High Throughput N-Glycan Analysis by Mass Spectrometry

 

July 01, 2016

Gly-X with InstantPC kits and standards now available!

We are pleased to announce the launch of our new Gly-X with InstantPC kits and InstantPC standards.

The new Gly-X with InstantPC kits combine a 5-minute in-solution enzymatic protein deglycosylation step followed by 1-minute labeling of released N-glycans with InstantPC dye. A simple and efficient clean-up step removes dye and other interfering compounds. Labeled glycans are ready for analysis by UHPLC, LC-MS, MS/MS. The Gly-X sample prep method is simple and rapid. InstantPC dye delivers unmatched fluorescent brightness, its small size contributes to improved HILIC resolution and a tertiary amine provides increased MS signal in positive mode for N-glycan identification.

 

Gly-X with InstantPC kits:

  • GX96-IPC Gly-X N-Glycan Rapid Release and Labeling with InstantPC Kit (96-ct)
  • GX24-IPC Gly-X N-Glycan Rapid Release and Labeling with InstantPC Kit (24-ct)

 

InstantPC Standards:

 For more information, please contact us

June 09, 2016

GlykoPrep-plus InstantPC poster presented at ASMS 2016, San Antonio

We were pleased to present a poster on our InstantPC glycan labeling dye at ASMS 2016 in San Antonio (June 5-9). The work utilized the GPPNG-PC kit, GlykoPrep-plus with InstantPC labeling, automated on the Agilent AssayMAP Bravo.

A handout version of our ASMS 2016 poster is available here:

Automated N-Glycan Sample Preparation with an Instant Glycan Labeling Dye for Mass Spectrometry 

 

May 02, 2016

GlykoPrep InstantPC, InstantAB and APTS kits for automation on the Agilent Bravo now available!

We are pleased to announce the launch of 3 new GlykoPrep kits for automated N-glycan sample preparation on the Agilent AssayMAP Bravo.

- GPPNG-PC, GlykoPrep-plus Rapid N-Glycan Sample Preparation with InstantPC is for the preparation of released, InstantPC-labeled N-glycans for separation by liquid chromatography with fluorescence detection and mass spectrometry. This new dye was specifically engineered for LC/MS analysis and provides exceptional response in both fluorescence and mass spectrometry applications.  Instant labeling allows for an automated workflow of around 3 hours!

 

- GPPNG-LB, GlykoPrep-plus Rapid N-Glycan Sample Preparation with InstantAB is for the preparation of released, InstantAB- labeled N-glycans for separation by liquid chromatography with fluorescence detection. Instant labeling allows for an automated workflow of around 3 hours!

 

- GPPNG-APTS, GlykoPrep-plus Rapid N-Glycan Sample Preparation with APTS (8-Aminopyrene-1,3,6-trisulfonic acid) is for the preparation of released, APTS-labeled glycans for separation by capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection. The workflow takes around 4-5 hours, including drydown of glycan samples and a 1 hour heated labeling step.

 

These 3 new products are in addition to our existing GlykoPrep-plus 2-AB kit, GPPNG-AB. InstantPCInstantAB, 2-AB and APTS GlykoPrep-plus kits are provided in a 96-count format with sufficient reagents to prepare 96 labeled N-glycan samples, in a single Bravo run or in 4 runs totaling 96 replicates.

For more information, please contact us

 

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