GlykoPrep® Rapid N-Glycan Preparation with InstantPC™ (96-ct) [GP96NG-PC]

Price:

Description

InstantPC dye is a novel instant glycan labeling reagent for MS that provides markedly increased MS and fluorescent sensitivity. InstantPC forms a stable urea linkage with glycosylamines released by rapid N-Glycanase (PNGase F) digestion. This GlykoPrep N-glycan sample preparation kit utilizes a rapid digestion, instant labeling, and cleanup of excess InstantPC label. 

Contains:

The GlykoPrep® platform dramatically streamlines glycoprotein sample preparation. From purification to glycan release and labeling, the entire sample prep can be completed in 3 hours.

    • Optional protein A purification for monoclonal antibodies and Fc proteins
    • Non-selective, rapid release and recovery of intact N-Glycans from up to 96 glycoprotein samples at a time using a microplate centrifuge
    • Non-selective chemistry for stoichiometric labeling of glycans, independent of structure
    • Flexible, high-throughput format: process 1 to 192 samples per run (2 kits simultaneously)
    • InstantDye™ labeling of N-glycans with InstantPC provides high fluorescence and MS signal
    • Labeled glycans are eluted in low volume and ready for analysis without concentrating or drying

The GlykoPrep Sample Preparation System requires minimal equipment. All you need is a centrifuge with a deep-well plate rotor (minimum 44 mm height), a Starter Labware Kit (AM200) and Heater and Incubation Blocks (GS150).

Size: 1 kit (96-ct)

Product Code: GP96NG-PC

Tech Info

GP96NG-PC GlykoPrep InstantPC Instruction Manual 

GP96NG-PC GlykoPrep InstantPC Microfuge Instruction Manual 

GlykoPrep Guidebook  

Publications
  1. Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection. Agilent Application Note using GlykoPrep with InstantPC, 2016.
  2. Development of an Instant Glycan Dye for High Throughput Analysis by Mass Spectrometry
Safety

Product Safety Documentation for GP96NG-PC:

Product/Part No. Description
GS96-RX  GlykoPrep Digestion Module 
WS0253 Digestion (RX) Cartridges
WS0256 Immobilization Reagent Set
WS0226 Denaturation Reagent
WS0255 Blocking Reagent
WS0259 Digestion Reagent Set
WS0229 Finishing Reagent
WS0276 25X Digestion Buffer
WS0278 N-Glycanase® (PNGase F)
WS0338 US-InstantPC™ Dye
WS0339 US-Dye Solvent
GS96-CU GlykoPrep Cleanup Module
WS0263 GlykoPrep Cleanup Module

 

Certificates of Analysis

This kit contains multiple modules, each with their own Certificate of Analysis, which may be searched for & accessed here.


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