Glyko® β (1-2,3,4,6) Hexosaminidase, Sequencing-grade [GKX-5023]

$ 500.00


Releases non-reducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Supplied at a higher concentration than GKX-5003. Higher enzyme concentrations may be necessary to remove bisecting β(1-4)-linked GlcNAc residues in complex N-glycans. 

Jack bean.

The enzyme exhibits a broad specificity, cleaving non-reducing terminal β(1-2,3,4, and 6)-linked-N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. It has proved very useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with β-N-Acetylhexosaminidase from S. Pneumoniae (GK80050).


Figure 1: Cleavage specificity for GKX-5023 β N-Acetylhexosaminidase.

Ships with:
1 ea WS0055 5x Enzyme Reaction Buffer (S6) (500mM sodium phosphate, pH 5.5)

20mM sodium citrate phosphate (pH 6.0)

Reaction Buffer:
5x Enzyme Reaction Buffer (S6) (500mM sodium phosphate, pH 5.5)

~67 kD (from literature)

pH optimum/range:

Unit Definition:
One unit of β-N-Acetylhexosaminidase is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from pNP-N-acetyl-β-D-glucosaminide per minute at pH 5.0 and 37°C.

Size: 15 U in 50µl

Concentration: ≥ 300 U/ml

Product Code: GKX-5023


Product Safety Documentation for GKX-5023:

Product/Part No. Description

US-Glyko® β (1-2,3,4,6) Hexosaminidase

GB-Glyko® β (1-2,3,4,6) Hexosaminidase


US-5x Reaction Buffer - Vial S6

GB-5x Reaction Buffer - Vial S6

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