Glyko® Sialidase V™ [GKX-5020]

Price:
$ 350.00

Description

Releases α(2-3,6,8)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates.

 

Figure 1: Cleavage specificity for GKX-5020 Sialidase V™.

Source:
Native from Vibrio cholerae.

Specificity:
The enzyme is specific for N- acetyl (Neu5Ac) or N-glycolyl (Neu5Gc) neuraminic acid at the non-reducing terminus in α2-3,6 or 8 linkages to various monosaccharides (e.g. galactose, neuraminic acid (NeuAc-NeuAc), N-acetyl-glucosamine, N-acetyl-galactosamine). These are found in a wide range of oligosaccharides, polysaccharides and glycoconjugates such as glycoproteins and glycolipids. The rate of hydrolysis of α2-3 over α2-6 and/or α2-8 linkages is approximately 3 fold.

Applications:
The purity and broad substrate specificity makes Vibrio cholerae sialidase suitable for oligosaccharide and glycoconjugate structure determination and for probing cell surface molecules (e.g. receptors).

Ships with:
WS0146 5x Reaction Buffer for GKX-5020 [250 mM sodium acetate, 20 mM calcium chloride, 0.5 mg/ml BSA (pH 5.5)

Formulation:
Sterile filtered solution in 50 mM sodium acetate, 154 mM NaCl, 9 mM CaCl2, 0.1% Micr-O-protect, 10 mM EDTA and ~100 μg/ml HSA (pH 5.5)

Reaction Buffer:
5X concentrated buffer which when diluted gives 50 mM sodium acetate pH 5.5, containing 4 mM CaCl2 and 0.1 mg/ml bovine serum albumin.

MW:
~83 kD

pH optimum/range:
5.5

Unit Definition:
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of methylumbelliferone from MU-NANA [2'-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid] per minute at pH 5.5 and 37°C.

Size: 1 U (200 µl)

Concentration: ≥ 5 U/ml

Product Code: GKX-5020

Safety

Product Safety Documentation for GKX-5020:

Product/Part No. Description
Glyko® Sialidase V™ [GKX-5020]

US-GKX-5020

GB-GKX-5020

 




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