Releases α(2-3,6,8)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates.
Figure 1: Cleavage specificity for GKX-5020 Sialidase V™.
Native from Vibrio cholerae.
The enzyme is specific for N- acetyl (Neu5Ac) or N-glycolyl (Neu5Gc) neuraminic acid at the non-reducing terminus in α2-3,6 or 8 linkages to various monosaccharides (e.g. galactose, neuraminic acid (NeuAc-NeuAc), N-acetyl-glucosamine, N-acetyl-galactosamine). These are found in a wide range of oligosaccharides, polysaccharides and glycoconjugates such as glycoproteins and glycolipids. The rate of hydrolysis of α2-3 over α2-6 and/or α2-8 linkages is approximately 3 fold.
The purity and broad substrate specificity makes Vibrio cholerae sialidase suitable for oligosaccharide and glycoconjugate structure determination and for probing cell surface molecules (e.g. receptors).
WS0146 5x Reaction Buffer for GKX-5020 [250 mM sodium acetate, 20 mM calcium chloride, 0.5 mg/ml BSA (pH 5.5)
Sterile filtered solution in 50 mM sodium acetate, 154 mM NaCl, 9 mM CaCl2, 0.1% Micr-O-protect, 10 mM EDTA and ~100 μg/ml HSA (pH 5.5)
5X concentrated buffer which when diluted gives 50 mM sodium acetate pH 5.5, containing 4 mM CaCl2 and 0.1 mg/ml bovine serum albumin.
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of methylumbelliferone from MU-NANA [2'-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid] per minute at pH 5.5 and 37°C.
Size: 1 U (200 µl)
Concentration: ≥ 5 U/ml
Product Code: GKX-5020
Product Safety Documentation for GKX-5020:
|Glyko® Sialidase V™ [GKX-5020]||