Releases non-reducing terminal α(1-3,4)-linked fucose from oligosaccharides.
The enzyme specifically cleaves non-reducing terminal α1-3 or α1-4 fucose residues.
After digestion with 0.2 mU/ml enzyme at a substrate concentration of 40 μM in 50 mM sodium acetate pH 5.0, 2 nmol of LNFPII is completely de-fucosylated. 3'fucosyllactose (2 nmol) was 65% de-fucosylated by 5 mU of a1-3/4 fucosidase during a 24hr incubation. However, under equivalent conditions, no fucose was release from 3' sialo-3-fucosyllactose. Higher enzyme concentrations (>2 mU/ml) are found to be required for the removal of α1-3 or α1-4 fucose residues from complex N-glycans. Complete hydrolysis of Fuc α1-3 GlcNAc from the outer arms of a di α1-3 fucosylated, di α1-2 fucosylated bi-antennary N-glycan isolated from Human parotid gland was obtained at a final substrate concentration of 1 mM and a final enzyme concentration of 3 mU/ml.
The enzyme can be used in the analysis of fucosylated O- and N-linked glycans during exoglycosidase sequencing It has also been used in the analysis and modification of blood group oligosaccharides since it is active towards the Lex antigen.
WS0062 5x Rxn Buffer [250 mM sodium acetate (pH 5.0)]
Lyophilized from 50 mM sodium acetate buffer, 3 mg/ml BSA (pH 5.0) [~150 μg BSA per vial]
5X concentrated buffer which when diluted gives 50 mM sodium acetate pH 5.0.
One unit of α(1-3,4) Fucosidase is defined as the amount of enzyme which will release 1 μmole of fucose from lacto-N-fucopentaose II per minute at pH 5.0 and 37°C. Note: the enzyme is not active on p-nitrophenyl-α-l-fucopyranoside.
Size: 200 µU lyophilized
Product Code: GKX-5019
Product Safety Documentation for GKX-5019:
from $ 125.00