Releases non-reducing terminal β(1-4)-linked mannose from oligosaccharides. The enzyme hydrolyzes non-reducing terminal β(1-4)- linked mannose residues with relative specificity; β1-3 and β1-6 linked mannose residues are reported to be hydrolyzed at much lower rates of 0.04 and 0.1 % respectively.
Figure 1: Cleavage specificity for GKX-5015 β-Mannosidase.
The enzyme can be used to determine the anomericity of non-reducing terminal mannose linkages
WS0121 5x Reaction Buffer [500 mM sodium citrate phosphate (pH 4.0)]
Reaction Buffer: 5X concentrated buffer which when diluted gives 100 mM sodium citrate/phosphate pH 4.0
Lyophilized from 20 mM sodium citrate/phosphate pH 6.0, containing 0.5 mg/ml bovine serum albumin.
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP-β-mannopyranoside per minute at pH 4.0 and 37°C.
Size: 1 U
Product Code: GKX-5015
Product Safety Documentation for GKX-5015: