Glyko® β-Mannosidase [GKX-5015]

Price:
$ 215.00

Description

Releases non-reducing terminal β(1-4)-linked mannose from oligosaccharides. The enzyme hydrolyzes non-reducing terminal β(1-4)- linked mannose residues with relative specificity; β1-3 and β1-6 linked mannose residues are reported to be hydrolyzed at much lower rates of 0.04 and 0.1 % respectively.

 

Figure 1: Cleavage specificity for GKX-5015 β-Mannosidase.

Source:
Helix pomatia.

Applications:
The enzyme can be used to determine the anomericity of non-reducing terminal mannose linkages

Ships with:
WS0121 5x Reaction Buffer [500 mM sodium citrate phosphate (pH 4.0)]

Reaction Buffer: 5X concentrated buffer which when diluted gives 100 mM sodium citrate/phosphate pH 4.0

    Formulation:
    Lyophilized from 20 mM sodium citrate/phosphate pH 6.0, containing 0.5 mg/ml bovine serum albumin.

    MW:
    ~94 kD

    pH optimum/range:
    4.0

    Unit Definition:
    One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP-β-mannopyranoside per minute at pH 4.0 and 37°C.

    Size: 1 U

    Product Code: GKX-5015

    Safety

    Product Safety Documentation for GKX-5015:

    Product/Part No. Description
    GKX-5015

    US-Glyko® β-Mannosidase (Helix pomatia)

    GB-Glyko® β-Mannosidase (Helix pomatia)

    

    WS0121

    US-5x Reaction Buffer

    GB-5x Reaction Buffer

     


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