Glyko® β(1-4)-Galactosidase [GKX-5014]

Price:
$ 200.00

Description

The enzyme releases non-reducing terminal β(1-4)-linked galactose from oligosaccharides and glycoproteins. This specificity is only evident at enzyme concentrations < 100mU/ml. At higher concentrations, hydrolysis of β(1-3)-linked galactose occurs.

 

Figure 1: Cleavage specificity for GKX-5014 β(1-4)-Galactosidase.

Source:
Streptococcus pneumoniae.

Applications:
Due to its high selectivity the enzyme is an extremely useful reagent for the identification of non-reducing terminal β(1-4)-linked galactose residues. As such the enzyme has been extensively used for detailed structural analysis in conjunction with broader specificity bovine testes β-galactosidase (GKX-5013) or Jack bean β-galactosidase (GKX-5012).

Suggestions for Use:
Procedure For De-galactosylation
1. Add up to 100 μg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.
2. Add de-ionized water to a total of 14 μl.
3. Add 4 μl of 5x Reaction Buffer.
4. Add 2 μl β(1-4) Galactosidase.
5. Incubate at 37 °C for 1 hour.

For glycoproteins, cleavage may be monitored by SDS-PAGE if the size differential between native and de-galactosylated protein is sufficient for detection.

For the cleavage of Galβ(1-4) linkages occurring in isolated glycans, the substrate reaction concentration should be approximately 10-20 μM. Reconstitute the enzyme in 1X Incubation buffer to give a reaction concentration of 80 mU/mL. Incubate for up to 18 hours at 37 °C.

Ships with:
WS0049 5x Reaction Buffer B [250 mM sodium phosphate (pH 6.0)]

Formulation:
20 mM Tris-HCl, 25 mM NaCl (pH 7.5)

Reaction Buffer:
5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 6.0.

MW: 220-247 kD

pH optimum/range:
6.0

Unit Definition:
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole oNP-β-D-galactopyranoside per min at pH 6.0 and 37°C.

Size: 200 mU (100 µl)

Concentration: ≥ 2 U/ml

Product Code: GKX-5014

Safety

Product Safety Documentation for GKX-5014:

Product/Part No. Description
GKX-5014 Glyko® β(1-4)-Galactosidase (Streptococcus pneumonia) 
WS0049 5X Reaction Buffer B

 

 

 

 

 

 

 

 


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