Hydrolyzes non-reducing terminal galactose β(1-3) and β(1-4) linkages. Can be used in conjunction with other β-galactosidases for exoglycosidase sequencing.
Figure 1: Cleavage specificity for GKX-5013 β(1-3,4)-Galactosidase.
The enzyme has applications in the analysis of a wide variety of glycoconjugates. It is particularly useful for ensuring the complete removal of β(1-3) and β(1-4)-linked non-reducing terminal galactose residues of oligosaccharides. Gal β(1-6) GlcNAc is hydrolyzed more slowly, however this linkage is not normally encountered in native complex glycans. This activity towards β(1-3) and β(1-4)-linked galactose contrasts with that of our other β-galactosidases (e.g. from S.pneumoniae, GKX-5014, or Jack bean, GKX-5012) which exhibit a preference for Gal β(1-4), and cleave the Gal β(1-3) linkage relatively slowly, if at all. Used in conjunction, these enzymes provide a powerful means to determine linkage positions of non-reducing β galactose residues.
WS0063 5x Reaction Buffer [500 mM sodium citrate/phosphate pH 4.0]
20 mM sodium citrate phosphate, 150 mM NaCl (pH 4.0)
5X concentrated buffer which when diluted gives 100 mM sodium citrate/phosphate pH 4.0.
MW: ~68 kD
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP-β-D-galactopyranoside per minute at pH 4.0 and 37°C.
Size: 0.5 U (100 μl)
Concentration: ≥ 5 U/ml
Product Code: GKX-5013
Product Safety Documentation for GKX-5013:
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