Glyko® β(1-4,6)-Galactosidase [GKX-5012]

Price:
$ 300.00

Description

Hydrolyses non-reducing terminal Galβ(1-6)GlcNAc and Galβ(1-4)GlcNAc. Galβ(1-3)GlcNAc is hydrolyzed very slowly. The relative rates of hydrolysis are: β(1-6), 100%; β(1-4) 75%; and β(1-3), 1%. Galβ(1-6) and Galβ(1-3) can also be released from other aglycones although the rates of hydrolysis are variable.

 

Figure 1: Cleavage specificity for GKX-5012 β(1-4,6)-Galactosidase.

Source:
Jack bean.

Applications:
The enzyme has been extensively used for the study of isolated glycans, glycolipids and glycoproteins.

Ships with:
WS0108 5x Reaction Buffer [250 mM sodium citrate phosphate (pH 3.5)]

Formulation:
Lyophilized from 20 mM sodium citrate/phosphate pH 6.0.

Reaction Buffer:
5X concentrated buffer which when diluted gives 50 mM sodium citrate pH 3.5.

MW: ~75 kDa

pH optimum/range:
3.5 - 4.5

Unit Definition:
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP-β-D-galactopyranoside per minute at pH 3.5 and 37°C.

Size: 5 U lyophilized

Product Code: GKX-5012

FAQs

Q. The product is supplied lyophilized. How do I resuspend it?

A. Instructions for reconstitution of GKX-5012 are contained in the Certificate of Analysis. Dissolve the lyophilizate in 200 μl of high purity water to obtain the described
formulation (20 mM sodium citrate phosphate, pH 6.0). Since the enzyme will be more concentrated than is required for most applications, dilute further with buffer as needed.

Product Citations
  1. Ravidà A, Aldridge AM, Driessen NN, Heus FA, Hokke CH, O'Neill SM.  Fasciola hepatica Surface Coat Glycoproteins Contain Mannosylated and Phosphorylated N-glycans and Exhibit Immune Modulatory Properties Independent of the Mannose Receptor.   2016 Apr 22;10(4):e0004601.
  2. Yu Y, Mishra S, Song X, Lasanajak Y, Bradley KC, Tappert MM, Air GM, Steinhauer DA, Halder S, Cotmore S, Tattersall P, Agbandje-McKenna M, Cummings RD, Smith DF.  Functional glycomic analysis of human milk glycans reveals the presence of virus receptors and embryonic stem cell biomarkers.   2012 Dec 28;287(53):44784-99.
  3. de Vries RP, Smit CH, de Bruin E, Rigter A, de Vries E, Cornelissen LA, Eggink D, Chung NP, Moore JP, Sanders RW, Hokke CH, Koopmans M, Rottier PJ, de Haan CA.  Glycan-dependent immunogenicity of recombinant soluble trimeric hemagglutinin.   2012 Nov;86(21):11735-44.
Safety

Product Safety Documentation for GKX-5012:

Product/Part No. Description
GKX-5012 Glyko® β(1-4,6)-Galactosidase (Jack bean) 
WS0108

US-5x Reaction Buffer

GB-5x Reaction Buffer


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