Glyko® β N-Acetylhexosaminidase [GKX-5003]

Price:
$ 200.00

Description

Releases non-reducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues.  

Source:
Jack bean.

Specificity:
The enzyme exhibits a broad specificity, cleaving non-reducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. This enzyme is very useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with β N-acetylhexosaminidase from S. pneumoniae (GK80050).

We also offer this enzyme at a higher concentration - see GKX-5023. Higher enzyme concentrations may be necessary to remove bisecting β(1-4)-linked GlcNAc residues in complex N-glycans.

 

Figure 1: Cleavage specificity for GKX-5003 β N-Acetylhexosaminidase.

Applications:
Useful for the structural analysis of isolated glycans and glycoconjugates.

Ships with:
WS0109 5x Incubation Buffer [500 mM sodium citrate phosphate (pH 5.0)]

Reaction Buffer:
5X concentrated buffer which when diluted gives 100 mM sodium citrate/phosphate pH 5.0.

Formulation:
20 mM sodium citrate phosphate, 0.25 mg/ml BSA, 0.02% sodium azide (pH 4.0)

MW: 100 - 110 kDa

pH optimum/range:
For glucosaminide 5.0 - 6.0
For galactosaminide 3.5 - 4.0

Unit Definition:
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP N-acetyl-β-D-glucosaminide per minute at pH 5.0 and 37°C.

Size: 5 U in 100 µl

Concentration: ≥ 50 U/ml

Product Code: GKX-5003

Safety

Product Safety Documentation for GKX-5003:

Product/Part No. Description
GKX-5003

US-Glyko® β N-Acetylhexosaminidase (jack bean)

GB-Glyko® β N-Acetylhexosaminidase (jack bean)



WS0109

US-5x Reaction Buffer

GB-5x Reaction Buffer


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