Glyko® 2-AB-α(2-3) Sialylated Triantennary Library consists of triantennary oligosaccharides containing α(2-3) linked sialic acid. The reducing termini are derivatized with the fluorescent dye, 2-aminobenzamide (2-AB).
Please visit the Structures tab for a list of glycans typically contained in this library, or contact us for a representative Certificate of Analysis.
The α(2-3) sialic acid linkage is found on glycoproteins produced in Chinese hamster ovary (CHO) cells . In contrast, human intravenous immunoglobulin (IVIG) IgG Fc N-glycans are predominantly α(2-6)-sialylated . Depending on the separation method, it may be possible to resolve α(2-3) and α(2-6) sialic acid linkage isomers. For example, α(2-3)-sialylated N-glycans are known to have a shorter HILIC retention time than isomeric N-glycans with α(2-6) sialic acid linkages [3,4]. Sialic acid linkage position may also be determined by exoglycosidase digests with Sialidase S (GK80021), which releases non-reducing terminal α(2-3)-linked sialic acid, and Sialidase A (GK80040) which releases α(2-3,6,8,9)-linked sialic acid.
Size: 200 pmol
Product Code: GKSB-233
The table below indicates the N-glycans typically contained in this library in order of HILIC retention time (shortest to longest) using QC HILIC methods. Due to variability in starting material, actual glycans identified in each lot may be subject to change. If you would like more information about our current lot(s), please contact us.
N-Glycans of 2-AB-α(2-3) Sialylated Triantennary Library:
|Glycan Identification||ProZyme||Oxford (New) ||Structure |
|Asialo, galactosylated triantennary||NA3||A3G(4)3|
|Mono-α(2-3)-sialylated, galactosylated triantennary||NA3S1||A3G(4)3S(3)1|
|Di-α(2-3)-sialylated, galactosylated triantennary||NA3S2||A3G(4)3S(3)2|
|Tri-α(2-3)-sialylated, galactosylated triantennary||A3||A3G(4)3S(3)3|
Structure Key :
Structures drawn using GlycoWorkbench.
Product Safety Documentation for GKSB-233:
|GKSB-233||Glyko® 2-AB-α(2-3) Sialylated Triantennary Library|||