Glyko® 2-AB-α(2-3) Sialylated Biantennary Library [GKSB-232]

Price:
$ 400.00

Description


The Glyko® 2-AB-α(2-3) Sialylated Biantennary Library consists of biantennary oligosaccharides containing α(2-3) linked sialic acid.  The reducing termini are derivatized with the fluorescent dye, 2-aminobenzamide (2-AB).

Please visit the Structures tab for a list of glycans typically contained in this library, or contact us for a representative Certificate of Analysis.

The α(2-3) sialic acid linkage is found on glycoproteins produced in Chinese hamster ovary (CHO) cells [1].  In contrast, human intravenous immunoglobulin (IVIG) IgG Fc N-glycans are predominantly α(2-6)-sialylated [2].  Depending on the separation method, it may be possible to resolve α(2-3) and α(2-6) sialic acid linkage isomers.  For example, α(2-3)-sialylated N-glycans are known to have a shorter HILIC retention time than isomeric N-glycans with α(2-6) sialic acid linkages [3,4].  Sialic acid linkage position may also be determined by exoglycosidase digests with Sialidase S (GK80021), which releases non-reducing terminal α(2-3)-linked sialic acid, and Sialidase A (GK80040) which releases α(2-3,6,8,9)-linked sialic acid.

Size: 200 pmol

Product Code: GKSB-232

References:

  1. Lee EU, Roth J, Paulson JC. Alteration of terminal glycosylation sequences on N-linked oligosaccharides of Chinese hamster ovary cells by expression of beta-galactoside alpha 2,6-sialyltransferase.  1989 Aug 15;264(23):13848-55.
  2. Anthony RM, Nimmerjahn F, Ashline DJ, Reinhold VN, Paulson JC, Ravetch JV. Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG Fc.  2008 Apr 18;320(5874):373-6. 
  3. Raymond C, Robotham A, Spearman M, Butler M, Kelly J, Durocher Y. Production of α2,6-sialylated IgG1 in CHO cells.  2015;7(3):571-83. 
  4. GlycoBase 3.2.4. [Internet]. Blackrock, Ireland: National Institute for Bioprocessing Research and Training (NIBRT); c2012. All Collections including digests (93); [cited 2016 Apr 12]. Available from: https://glycobase.nibrt.ie/glycobase/browse_glycans.action
Structures

The table below indicates the N-glycans typically contained in this library in order of HILIC retention time (shortest to longest) using QC HILIC methods.  Due to variability in starting material, actual glycans identified in each lot may be subject to change. If you would like more information about our current lot(s), please contact us.

N-Glycans of 2-AB-α(2-3) Sialylated Biantennary Library

Glycan Identification ProZyme Common Oxford (New) [1] Structure [2]
Asialo, mono-galactosylated biantennary NA2G1 G1[6] A2[6]G(4)1

Asialo, mono-galactosylated biantennary

NA2G1 G1[3] A2[3]G(4)1
Asialo, mono-galactosylated biantennary, core substituted with fucose NA2G1F G1F[6] F(6)A2[6]G(4)1
Asialo, mono-galactosylated biantennary, core substituted with fucose NA2G1F G1F[3] F(6)A2[3]G(4)1
Asialo, galactosylated biantennary NA2 G2 A2G(4)2
Mono-α(2-3)-sialylated, mono-galactosylated biantennary NA2G1S1 G1[6]S1 A2[6]G(4)1S(3)1
Mono-α(2-3)-sialylated, mono-galactosylated biantennary NA2G1S1 G1[3]S1 A2[3]G(4)1S(3)1
Asialo, galactosylated biantennary core substituted with fucose NA2F G2F F(6)A2G(4)2

Mono-α(2-3)-sialylated, mono-galactosylated biantennary, core substituted with fucose

NA2G1FS1 G1F[6]S1 F(6)A2[6]G(4)1S(3)1

Mono-α(2-3)-sialylated, mono-galactosylated biantennary, core substituted with fucose

NA2G1FS1 G1F[3]S1 F(6)A2[3]G(4)1S(3)1
Mono-α(2-3)-sialylated, galactosylated biantennary A1 G2S1 A2G(4)2S(3)1
Mono-α(2-3)-sialylated, galactosylated biantennary, core substituted with fucose A1F G1FS1 F(6)A2G(4)2S(3)1
Di-α(2-3)-sialylated, galactosylated biantennary A2 G2S2 A2G(4)2S(3)2
Di-α(2-3)-sialylated, galactosylated biantennary, core substituted with fucose A2F G2FS2 F(6)A2G(4)2S(3)2

Structure Key [2]:

Structures drawn using GlycoWorkbench[3]. 

References:

  1. GlycoBase 3.2.4. [Internet]. Blackrock, Ireland: National Institute for Bioprocessing Research and Training (NIBRT); c2012. Guide to the abbreviations used to identify 2AB labelled N-glycans; [cited 2016 Apr 19].  Available from: www.glycobase.nibrt.ie/glycobase/documents/abbreviations.pdf
  2. Harvey DJ, Merry AH, Royle L, Campbell MP, Dwek RA, Rudd PM. Proposal for a standard system for drawing structural diagrams of N- and O-linked carbohydrates and related compounds.  2009 Aug;9(15):3796-801
  3. Ceroni A, Maass K, Geyer H, Geyer R, Dell A, Haslam SM. GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans.  2008 Apr;7(4):1650-9.
Safety

Product Safety Documentation for GKSB-232:

Product/Part No. Description
GKSB-232 Glyko® 2-AB-α(2-3) Sialylated Biantennary Library 

 


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