Glyko® InstantAB Biantennary & High Mannose Partitioned Library [GKIB-520]

Price:
$ 925.00

Description

The Glyko® InstantAB-(Biantennary & High Mannose Partitioned Library) consists of 3 blended libraries of N-linked glycans whose reducing termini are derivatized with the fluorescent dye, InstantAB. The libraries were partitioned to minimize overlap of peaks to facilitate glycan peak identification. See "Structures" tab for a list of glycans contained within typical lots of this library.

Pack includes:

  • WS0330 InstantAB-(Fucosyl Biantennary Library), 1 vial (70 pmol)
  • WS0331 InstantAB-(Afucosyl Biantennary Library), 1 vial (70 pmol)
  • WS0332 InstantAB-(High Mannose Library), 1 vial (70 pmol)

Please contact us for a representative Certificate of Analysis.

Application: qualitative standards for glycan identification in applications such as hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-FLD).

Size: 3 x 70 pmol libraries

Product Code: GKIB-520

Structures

Below are tables indicating the N-glycans typically contained in these libraries. Due to variability in starting material, actual glycans identified in each lot may be subject to change. If you would like more information about our current lot(s), please contact us.

Table 1 - N-Glycans of InstantAB Fucosyl Biantennary Library

Table 2 - N-Glycans of InstantAB Afucosyl Biantennary Library

Table 3 - N-Glycans of InstantAB High Mannose Library

Table 1 - N-Glycans of InstantAB Fucosyl Biantennary Library (WS0330)

Glycan Identification ProZyme Common Oxford (New) [1] Structure [2]
6-α-fucosyl-N,N'-diacetylchitobiose N2F N/A N/A
4-β-mannosyl N,N'-diacetylchitobiose with core fucose MNNF N/A N/A
Conserved trimannosyl core with core fucose M3N2F N/A F(6)M3
Asialo-, agalacto- biantennary NGA2 G0 A2
Asialo-, agalacto- biantennary with core fucose NGA2F G0F F(6)A2
Asialo-, agalacto- biantennary with core fucose and with bisecting N-Acetylglucosamine NGA2FB G0FB F(6)A2B
Asialo-, mono-galacto- biantennary NA2G1 G1 A2G(4)1
Asialo-, mono-galacto- biantennary with core fucose NA2G1F G1F[6] F(6)A2[6]G(4)1
Asialo-, mono-galacto- biantennary with core fucose NA2G1F G1F[3] F(6)A2[3]G(4)1
Asialo-, mono-galactosylated biantennary with core fucose and bisecting N-Acetylglucosamine NA2G1FB G1FB[6] F(6)A2B[6]G(4)1
Asialo-, galactosylated biantennary NA2 G2 A2G(4)2
Asialo-, galactosylated biantennary with core fucose NA2F G2F F(6)A2G(4)2
Asialo-, galactosylated biantennary with core fucose and bisecting N-Acetylglucosamine NA2FB G2FB F(6)A2BG(4)2
Mono-sialylated, mono-galactosylated, biantennary with core fucose NA2G1FS1 G1FS1 F(6)A2[3]G(4)1S(6)1
Mono-sialylated, galactosylated biantennary A1 G2S1 A2G(4)2S(6)1
Mono-sialylated, galactosylated biantennary, with core fucose A1F G2FS1 F(6)A2G(4)2S(6)1
Mono-sialylated, galactosylated biantennary, with core fucose and bisecting N-Acetylglucosamine A1FB G2FBS1 F(6)A2BG(4)2S(6)1
Di-sialylated, galactosylated biantennary A2 G2S2 A2G(4)2S(6)2
Di-sialylated, galactosylated biantennary with core fucose A2F G2FS2 F(6)A2G(4)2S(6)2
Di-sialylated, galactosylated biantennary with core fucose and bisecting N-Acetylglucosamine A2FB G2FBS2 F(6)A2BG(4)2S(6)2

Table 2 - N-Glycans of InstantAB Afucosyl Biantennary Library (WS0331)

Glycan Identification ProZyme Common Oxford (New) [1] Structure [2]
Chitobiose N2 N/A N/A
4-β-mannosyl chitobiose MNN N/A N/A
Conserved trimannosyl core M3N2 N/A M3
Asialo-, agalacto- biantennary NGA2 G0 A2
Asialo-, agalacto- biantennary with bisecting N-Acetylglusosamine NGA2B G0B A2B
Asialo-, mono-galacto- biantennary NA2G1 G1[6] A2[6]G(4)1
Asialo-, mono-galacto- biantennary NA2G1 G1[3] A2[3]G(4)1
Asialo-, mono-galactosylated biantennary with bisecting N-Acetylglucosamine NA2G1B G1B A2BG(4)1
Asialo-, galactosylated biantennary NA2 G2 A2G(4)2
Asialo-, galactosylated biantennary with bisecting N-Acetylglucosamine NA2B G2B A2BG2
Mono-sialylated, mono-galactosylated, biantennary NA2G1S1 G1S1 A2[3]G(4)1S(6)1
Mono-sialylated, galactosylated biantennary A1 G2S1 A2G(4)2S(6)1
Mono-sialylated, galactosylated biantennary with bisecting N-Acetylglucosamine A1B G2S1B A2BG(4)2S(6)1
Di-sialylated, galactosylated biantennary A2 G2S2 A2G(4)2S(6)2
Di-sialylated, galactosylated biantennary with bisecting N-Acetylglucosamine A2B G2S2B A2BG(4)2S(6)2

Table 3 - N-Glycans of InstantAB High Mannose Library (WS0332)

Glycan Identification ProZyme Common Oxford (New) [1] Structure [2]
Oligomannose 5 Man5 Man5 M5
Oligomannose 6 Man6 Man6 M6
Oligomannose 7 Man7 Man7 M7
Oligomannose 8 Man8 Man8 M8
Oligomannose 9 Man9 Man9 M9

 

Structure Key [2]:

Structures drawn using GlycoWorkbench [3]. 

  1. GlycoBase 3.2.4. [Internet]. Blackrock, Ireland: National Institute for Bioprocessing Research and Training (NIBRT); c2012. Guide to the abbreviations used to identify 2AB labelled N-glycans; [cited 2016 Apr 19].  Available from: www.glycobase.nibrt.ie/glycobase/documents/abbreviations.pdf
  2. Harvey DJ, Merry AH, Royle L, Campbell MP, Dwek RA, Rudd PM. Proposal for a standard system for drawing structural diagrams of N- and O-linked carbohydrates and related compounds.  2009 Aug;9(15):3796-801
  3. Ceroni A, Maass K, Geyer H, Geyer R, Dell A, Haslam SM. GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans.  2008 Apr;7(4):1650-9.

Safety

Product Safety Documentation for GKIB-520:

Product/Part No. Description
GKIB-520 Glyko® InstantAB Biantennary & High Mannose Partitioned Library 

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