Glyko® Endo F2 [GKE-5008]

Price:
$ 250.00

Description

Cleaves within the chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-glycans. Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. 

Source:
Recombinant gene from Elizabethkingia meningosepticum, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum1

Specificity:
The enzyme cleaves within the di-N-acetyl chitobiose  (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides.

 

Figure 1: Cleavage specificity for GKE-5008 Endo F2.

R1: N & C substitution other than H
R2: may be H or the rest of an oligosaccharide
R3: may be H or α(1-6)-linked fucose

Applications:
Selective release of high mannose and biantennary complex type N-linked oligosaccharides from glycopeptides/ glycoproteins.

Ships with:
WS0161 5X Reaction Buffer, which when diluted gives 200 mM sodium acetate pH 4.5.

Formulation:
10 mM sodium acetate pH 4.5, 25 mM NaCl, 0.02% sodium azide

pH optimum:
4.5

Unit Definition:
One milliunit is defined as the amount of enzyme that will hydrolyze 1 nmole of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 and 37°C.

Size: 6 mU (60 µl)

Concentration: ≥ 100 mU/ml

Product Code: GKX-5008

1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST. Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov.  2005 May;55(Pt 3):1287-93.

Tech Info

EC Number: 3.2.1.96

CAS Number: 37278-88-9

Database entries:

BRENDA | ExPASy | ExplorEnz | IntEnzIUBMB | KEGG | MetaCyc | PDB

 

Safety

Product Safety Documentation for GKE-5008:

Product/Part No. Description
GKE-5008

US-Glyko® Endo F2 

GB-Glyko® Endo F2



WS0161

US-5X Reaction Buffer

GB-5X Reaction Buffer

 


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