Native PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Also available as a recombinant product, see GKE-5006A
Native, purified from Elizabethkingia meningosepticum, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum1.
PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core α1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.
52 mM Tris-HCl, 36 mM NaCl, 9 mM sodium acetate, 5 mM EDTA, 0.02% sodium azide (pH 7.5), glycerol-free formulation
One unit of N-Glycanase is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at pH 7.5 and 37̊C.
Size: 100 mU (50 µl)
Product Code: GKE-5003
EC Number: 18.104.22.168
CAS Number: 83534-39-8
Product Safety Documentation for GKE-5003:
|GKE-5003||Glyko® PNGase F (native)|
|WS0067||5x Incubation Buffer|