Glyko® PNGase F (native), 100 mU [GKE-5003]

Price:
$ 200.00

Description

Native PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn.  Also available as a recombinant product, see GKE-5006A.

Source:
Native, purified from Elizabethkingia meningosepticum. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum [1]. 

Specificity:
PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core α1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.

 

Figure 1: Cleavage specificity for GKE-5003 N-Glycanase (PNGase F).
R1: N & C substitution other than H
R2: may be H or the rest of an oligosaccharide
R3: may be H or α(1-6)-linked fucose

Applications:

  • release of intact N-linked glycans from glycopeptides and glycoproteins
  • structure-function studies of N-glycosylated glycoproteins
  • preparation of deglycosylated proteins for molecular weight estimation or crystallography studies

Ships with: 

    • 1 ea WS0067 5x Reaction Buffer
    • 1 ea WS0012 Denaturation Solution
    • 1 ea WS0013 Detergent Solution

Formulation:
52 mM Tris-HCl, 36 mM NaCl, 9 mM sodium acetate, 5 mM EDTA, 0.02% sodium azide (pH 7.5), glycerol-free formulation

pH optimum:
7.5

Unit Definition:
One unit of N-Glycanase is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at pH 7.5 and 37̊C.

Size: 100 mU (50 µl)

Concentration: ≥ 2 U/ml

Product Code: GKE-5003

1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST. Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov.  2005 May;55(Pt 3):1287-93.

Tech Info

EC Number: 3.5.1.52

CAS Number: 83534-39-8

Database entries:

BRENDA | ExPASy | ExplorEnz | IntEnzIUBMB | KEGG | MetaCyc | PDB

Safety

Product Safety Documentation for GKE-5003:

Product/Part No. Description
GKE-5003

US-Glyko® PNGase F (native)

GB-Glyko® PNGase F (native)

WS0067 5x Incubation Buffer
WS0012 Denaturation
WS0013 Detergent Solution

 


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