Contains all enzymes & reagents needed to remove all N-linked glycans & simple O-linked glycans (including polysialylated) from glycoproteins. See the product manual for an example using bovine fetuin.
N-Glycanase (PNGase F) cleaves all N-linked complex, hybrid or high mannose oligosaccharides unless α(1,3) core fucosylated (asparagine must be peptide-bonded at both termini).
Unfortunately there is no such “universal” endoglycosidase for O-glycans, these have to be trimmed down to Gal-GalNAc-O-S/T before O-glycanase (GK80090) can remove the Gal-GalNAc structure. Modification of this core with e.g. sialic acid will block O-glycanase.
Sialidase A (GK80040) is included in the kit and can remove terminal sialic acid from sialylated T antigen/Core 1 (Sia-Gal-GalNAc-O-S/T) to reveal core 1 Gal-GalNAc. The GK80115 extender kit (β(1,4)Galactosidase & β-N-Acetylglucosaminidase), available separately, can assist with removal of e.g. core 2 structures (GlcNAc-Gal-GalNAc-S/T). However, for more extended & complex O-glycans structures these enzymes will not be sufficient to reveal Gal-GalNAc. For more on O-GalNAc glycan core structures, please see reference .
Size: 1 kit (~2 mg of glycoprotein)
Product Code: GK80110
Product Safety Documentation for GK80110:
|GK80110||GlycoPro™ Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans|||
|WS0041||N-Glycanase® PNGase F|
|WS0046||5x Reaction Buffer|