Releases unsubstituted, Ser/Thr-linked GalGalNAc from proteins; used with additional enzymes to remove more complex O-linked structures. Also known as Endo-O-Glycosidase™, Endo-α N-acetylgalactosaminidase, O-Glycosidase.
Recombinant gene from Streptococcus pneumoniae, expressed in E.coli.
The enzyme is highly specific, liberating Galβ1-3GalNAc from serine or threonine residues alone or as part of glycopeptides or glycoproteins. There is no apparent preference for serine over threonine. Substitution of the disaccharide core with sialic acid, fucose, N-acetylglucosamine or N-acetylgalactosamine residues, prevents cleavage of the glycosylamine linkage. Absence of the galactose residue or the acetamido group on the innermost N-acetylgalactosamine residue also prevents cleavage of the glycosylamine linkage.
O-Glycanase is included in our Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans (GK80110), which may be used in conjunction with our prO-LINK Extender™ Kit for Complex O-Linked Glycans (GK80115).
WS0059 5X reaction buffer A [250 mM sodium phosphate pH 5.0]
20 mM Tris HCl pH 7.5 containing 25 mM NaCl.
5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 5.0.
5.5 / 5.0 - 7.0
One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of Galβ1-3GalNAcα1-p-nitrophenol per minute at 37°C, pH 5.5.
Size: 50 mU (40 µl)
Concentration: ≥ 1.25 U/ml
Product Code: GK80090
Product Safety Documentation for GK80090:
|WS0059||5x Incubation Buffer A|
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