Glyko® Sialidase C™ [GK80030]

Price:
$ 270.00

Description

Releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates.

Source:
Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24.  

Specificity:
This enzyme releases α2-3, and α2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc α2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. For this application Sialidase A (GK80040) is recommended.  

 

Figure 1: Cleavage specificity for GK80030 Sialidase C.

Ships with (retail pack):
WS0049 5x Reaction Buffer B [250 mM sodium phosphate pH 6.0]

Reaction Buffer:
5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 6.0.

Formulation:
20 mM Tris HCl pH 7.5, containing 25 mM NaCl

pH optimum/range:
6.0

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5.

Size: 1 U (100 µl)

Concentration: ≥ 10 U/ml

Product Code: GK80030

Tech Info

GK80030 Technical Data Sheet  

Product Citations
  1. Umemoto E, Tanaka T, Kanda H, Jin S, Tohya K, Otani K, Matsutani T, Matsumoto M, Ebisuno Y, Jang MH, Fukuda M, Hirata T, Miyasaka M. Nepmucin, a novel HEV sialomucin, mediates L-selectin-dependent lymphocyte rolling and promotes lymphocyte adhesion under flow.  2006 Jun 12;203(6):1603-14.
Safety

Product Safety Documentation for GK80030:

Product/Part No. Description
GK80030 Glyko® Sialidase C™ 
WS0049 5x Reaction Buffer B 

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