Glyko® Sialidase C™ [GK80030]

Price:
$ 270.00

Description

Releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates.

Source:
Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24.  

Specificity:
This enzyme releases α2-3, and α2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc α2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. For this application Sialidase A (GK80040) is recommended.  

 

Figure 1: Cleavage specificity for GK80030 Sialidase C.

Ships with (retail pack):
WS0049 5x Reaction Buffer B [250 mM sodium phosphate pH 6.0]

Reaction Buffer:
5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 6.0.

Formulation:
20 mM Tris HCl pH 7.5, containing 25 mM NaCl

pH optimum/range:
6.0

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5.

Size: 1 U (100 µl)

Concentration: ≥ 10 U/ml

Product Code: GK80030

Tech Info

GK80030 Technical Data Sheet  

Safety

Product Safety Documentation for GK80030:

Product/Part No. Description
GK80030 Glyko® Sialidase C™ 
WS0049 5x Reaction Buffer B 

Related Items

Glyko® Sialidase A™ [GK80040]

$ 250.00

Releases α(2,3)-, α(2,6)-, α(2,8)-, and α(2,9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. Also capable of releasing N-glycolylneuraminic...

Glyko® Sialidase A™ 51 [GK80045]

$ 250.00

Lower MW version of GK80040 for challenging digestions. Releases α(2-3)-, α(2-6)-, α(2-8), and α(2-9)-linked...

Glyko® Sialidase A™ 66 [GK80046]

$ 250.00

Lower MW version of GK80040 for challenging digestions. Releases α(2-3)-, α(2-6)-, α(2-8), and α(2-9)-linked...