Releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates.
Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24.
This enzyme releases α2-3, and α2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc α2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. For this application Sialidase A (GK80040) is recommended.
Figure 1: Cleavage specificity for GK80030 Sialidase C.
Ships with (retail pack):
WS0049 5x Reaction Buffer B [250 mM sodium phosphate pH 6.0]
5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 6.0.
20 mM Tris HCl pH 7.5, containing 25 mM NaCl
One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5.
Size: 1 U (100 µl)
Concentration: ≥ 10 U/ml
Product Code: GK80030
Product Safety Documentation for GK80030:
|GK80030||Glyko® Sialidase C™|||
|WS0049||5x Reaction Buffer B|