What do I need for 2-AB glycan profiling?

What Glyko products do I need for glycan profiling of an antibody by HPLC using 2-AB fluorescent label?

ProZyme recommends using our GlykoPrep® Rapid Sample Preparation Platform for maximum throughput and reproducibility. For those wishing to purchase individual reagents to use with more traditional methods of glycan profiling, we recommend the following:


Various glycan standards appropriate for monoclonals are:

These standards are also available unlabeled for validation of the 2-AB labeling step. These are also useful as standards for analysis of the cleaved glycans with MALDI-TOF mass spectrometry:

N-Glycanase® is a registered trademark of Genzyme Corporation, Boston, MA, USA. Recombinant PNGase F is covered by US Patent No 5,238,821, which is exclusively licensed for manufacture by ProZyme, Inc.